NL2026932B1 - Preparation method for zein-degrading protease and application thereof - Google Patents

Preparation method for zein-degrading protease and application thereof

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Publication number
NL2026932B1
NL2026932B1 NL2026932A NL2026932A NL2026932B1 NL 2026932 B1 NL2026932 B1 NL 2026932B1 NL 2026932 A NL2026932 A NL 2026932A NL 2026932 A NL2026932 A NL 2026932A NL 2026932 B1 NL2026932 B1 NL 2026932B1
Authority
NL
Netherlands
Prior art keywords
zein
degrading protease
preparation
application
degrading
Prior art date
Application number
NL2026932A
Inventor
Pan Ke
Li Yanjiao
Qu Mingren
Liu Chanjuan
Zhang Wenjing
Zhao Xianghui
Yang Zhuqing
Original Assignee
Univ Jiangxi Agricultural
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Jiangxi Agricultural filed Critical Univ Jiangxi Agricultural
Application granted granted Critical
Publication of NL2026932B1 publication Critical patent/NL2026932B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention discloses a preparation method for a zein-degrading protease and an application thereof, and belongs to the technical field of molecular biology. The preparation method for a zein-degrading protease disclosed by the present invention successfully optimizes the gene sequence of a zein-degrading protease by using modern molecular technology, and constructs an expression vector pPlCZdA-ZDP that can express the zein-degrading protease in vitro outside yeast; and the zein-degrading protease can be prepared by using the vector and Pichia pastoris, thereby providing technical support for the application of the zein-degrading protease in production.
NL2026932A 2020-08-22 2020-11-19 Preparation method for zein-degrading protease and application thereof NL2026932B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010852694.7A CN112048518B (en) 2020-08-22 2020-08-22 Preparation method and application of zein degrading enzyme

Publications (1)

Publication Number Publication Date
NL2026932B1 true NL2026932B1 (en) 2021-07-20

Family

ID=73599564

Family Applications (1)

Application Number Title Priority Date Filing Date
NL2026932A NL2026932B1 (en) 2020-08-22 2020-11-19 Preparation method for zein-degrading protease and application thereof

Country Status (2)

Country Link
CN (1) CN112048518B (en)
NL (1) NL2026932B1 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002064760A2 (en) * 2001-02-09 2002-08-22 Roche Diagnostics Gmbh Expression of recombinant proteinase k from tritirachium album in yeast
WO2008033995A2 (en) * 2006-09-15 2008-03-20 Monsanto Technology Llc. Methods for increasing the fermentability of plant material to yield ethanol
CN101096696B (en) * 2007-07-06 2010-08-25 山东天久生物技术有限公司 Industrial production method of corn protein polypeptide from corn protein powder by enzymatical process
WO2011072191A2 (en) * 2009-12-11 2011-06-16 Novozymes A/S Protease variants
WO2018057420A1 (en) * 2016-09-23 2018-03-29 Dupont Nutrition Biosciences Aps Use of low ph active alpha-1,4/1,6-glycoside hydrolases as a feed additive for ruminants to enhance starch digestion

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1017595A (en) * 1996-07-01 1998-01-20 Sanei Touka Kk Modified zein and its production
GB0426160D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
CN102505034B (en) * 2011-12-02 2013-08-07 安徽燕之坊食品有限公司 Method for preparing active polypeptide from corn protein powder
CN105063138A (en) * 2015-09-14 2015-11-18 河南省科学院生物研究所有限责任公司 Method for producing trametes versicolor immunomodulatory protein by virtue of pichia pastoris expression system
CN110029123A (en) * 2019-04-03 2019-07-19 江西农业大学 The method and application of Yeast expression carrier building and expression circumscribed-type cellulase

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002064760A2 (en) * 2001-02-09 2002-08-22 Roche Diagnostics Gmbh Expression of recombinant proteinase k from tritirachium album in yeast
WO2008033995A2 (en) * 2006-09-15 2008-03-20 Monsanto Technology Llc. Methods for increasing the fermentability of plant material to yield ethanol
CN101096696B (en) * 2007-07-06 2010-08-25 山东天久生物技术有限公司 Industrial production method of corn protein polypeptide from corn protein powder by enzymatical process
WO2011072191A2 (en) * 2009-12-11 2011-06-16 Novozymes A/S Protease variants
WO2018057420A1 (en) * 2016-09-23 2018-03-29 Dupont Nutrition Biosciences Aps Use of low ph active alpha-1,4/1,6-glycoside hydrolases as a feed additive for ruminants to enhance starch digestion

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
"GenBank", Database accession no. NM_001137099.2
CAROL SODERLUND ET AL: "Sequencing, Mapping, and Analysis of 27,455 Maize Full-Length cDNAs", PLOS GENETICS, vol. 5, no. 11, 20 November 2009 (2009-11-20), pages e1000740, XP055066952, ISSN: 1553-7390, DOI: 10.1371/journal.pgen.1000740 *
DATABASE GenBank [online] NCBI; 30 June 2020 (2020-06-30), "Zea mays uncharacterized LOC100191670 (LOC100191670), mRNA", XP002802844, Database accession no. NM_001137099.2 *
DATABASE UniProt [online] 12 August 2020 (2020-08-12), "SubName: Full=KDEL-tailed cysteine endopeptidase CEP1 {ECO:0000313|EMBL:ONM35394.1};", XP002802845, retrieved from EBI accession no. UNIPROT:A0A1D6N408 Database accession no. A0A1D6N408 *
DE BARROS EVERALDO G. ET AL: "Purification and Characterization of Zein-Degrading Proteases from Endosperm of Germinating Maize Seeds", PLANT PHYSIOLOGY, vol. 94, no. 1, 1 September 1990 (1990-09-01), Rockville, Md, USA, pages 297 - 303, XP055799571, ISSN: 0032-0889, Retrieved from the Internet <URL:http://www.plantphysiol.org/content/plantphysiol/94/1/297.full-text.pdf> [retrieved on 20210428], DOI: 10.1104/pp.94.1.297 *
DEBARROS E G ET AL: "Cloning of a cDNA encoding a putative cysteine protease from germinating maize seeds", PLANT SCIENCE, ELSEVIER IRELAND LTD, IE, vol. 99, no. 2, 1 January 1994 (1994-01-01), pages 189 - 197, XP025220101, ISSN: 0168-9452, [retrieved on 19940101], DOI: 10.1016/0168-9452(94)90176-7 *
ZHANG WENJING ET AL: "Characterization of a recombinant zein-degrading protease from Zea mays by Pichia pastoris and its effects on enzymatic hydrolysis of corn starch", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, ELSEVIER BV, NL, vol. 164, 1 September 2020 (2020-09-01), pages 3287 - 3293, XP086334620, ISSN: 0141-8130, [retrieved on 20200901], DOI: 10.1016/J.IJBIOMAC.2020.08.237 *

Also Published As

Publication number Publication date
CN112048518B (en) 2022-06-24
CN112048518A (en) 2020-12-08

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NL2026932B1 (en) Preparation method for zein-degrading protease and application thereof