NL2005116C2 - Overbrenging van een monsterdrager in de correlatieve elektronenmicroscopie. - Google Patents
Overbrenging van een monsterdrager in de correlatieve elektronenmicroscopie. Download PDFInfo
- Publication number
- NL2005116C2 NL2005116C2 NL2005116A NL2005116A NL2005116C2 NL 2005116 C2 NL2005116 C2 NL 2005116C2 NL 2005116 A NL2005116 A NL 2005116A NL 2005116 A NL2005116 A NL 2005116A NL 2005116 C2 NL2005116 C2 NL 2005116C2
- Authority
- NL
- Netherlands
- Prior art keywords
- holding device
- containment
- sample carrier
- sample
- light microscope
- Prior art date
Links
- 238000001493 electron microscopy Methods 0.000 title claims description 18
- 230000005540 biological transmission Effects 0.000 title description 3
- 238000000034 method Methods 0.000 claims description 15
- 238000004113 cell culture Methods 0.000 claims description 12
- 230000008878 coupling Effects 0.000 claims description 12
- 238000010168 coupling process Methods 0.000 claims description 12
- 238000005859 coupling reaction Methods 0.000 claims description 12
- 230000000717 retained effect Effects 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 67
- 238000012546 transfer Methods 0.000 description 14
- 239000006059 cover glass Substances 0.000 description 11
- 239000007788 liquid Substances 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 10
- 238000007710 freezing Methods 0.000 description 7
- 230000008014 freezing Effects 0.000 description 7
- 239000000969 carrier Substances 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 239000012472 biological sample Substances 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- CNQCVBJFEGMYDW-UHFFFAOYSA-N lawrencium atom Chemical compound [Lr] CNQCVBJFEGMYDW-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000005452 bending Methods 0.000 description 2
- 230000031018 biological processes and functions Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000004627 transmission electron microscopy Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000002826 coolant Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012520 frozen sample Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 229910052594 sapphire Inorganic materials 0.000 description 1
- 239000010980 sapphire Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT0114409A AT508017B1 (de) | 2009-07-22 | 2009-07-22 | Transfer eines probenträgers in der korrelativen elektronenmikroskopie |
| AT11442009 | 2009-07-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NL2005116A NL2005116A (nl) | 2011-01-25 |
| NL2005116C2 true NL2005116C2 (nl) | 2012-01-24 |
Family
ID=42937621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL2005116A NL2005116C2 (nl) | 2009-07-22 | 2010-07-20 | Overbrenging van een monsterdrager in de correlatieve elektronenmicroscopie. |
Country Status (3)
| Country | Link |
|---|---|
| AT (1) | AT508017B1 (de) |
| DE (1) | DE102010021312B4 (de) |
| NL (1) | NL2005116C2 (de) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105652430A (zh) * | 2016-03-11 | 2016-06-08 | 张雪燕 | 一种生物实验室显微操作仪 |
| CN110927951B (zh) * | 2019-12-13 | 2021-09-14 | 北京大学深圳医院 | 一种新型玻璃化冷冻复融观察装置及其应用 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1886714U (de) * | 1963-11-12 | 1964-01-30 | Werner Dr Ing Schiebel | Pinzettenhaltevorrichtung. |
| DE3332741C2 (de) * | 1983-09-10 | 1986-09-04 | C. Reichert Optische Werke Ag, Wien | Vorrichtung zur Beleuchtung der Probe und Probenhalterung sowie des Kühlbads an einer Einrichtung zur Immersions-Kryofixation |
| FR2577690B1 (fr) * | 1985-02-21 | 1989-02-17 | Centre Nat Rech Scient | Surplatine pour etudes thermodynamiques sous microscope |
| DE3532606C1 (de) * | 1985-09-12 | 1986-11-13 | C. Reichert Optische Werke Ag, Wien | Vorrichtung zur Metallspiegel-Kryofixation von insbesondere biologischen Objekten |
| US4707998A (en) * | 1986-12-03 | 1987-11-24 | The Board Of Regents, The University Of Texas | Apparatus and method for ultrarapid cooling of biological samples |
| US5698856A (en) * | 1996-08-05 | 1997-12-16 | Frasca; Peter | Specimen holder for electron microscope |
| DE19731454A1 (de) * | 1997-07-22 | 1999-03-04 | Storz Karl Gmbh & Co | Chirurgische Faß- und Haltezange |
| WO1999043994A1 (en) * | 1998-02-26 | 1999-09-02 | Lucid, Inc. | Confocal microscope for facilitating cryosurgery of tissue |
| NL1017669C2 (nl) * | 2001-03-22 | 2002-09-24 | Univ Maastricht | Inrichting voor het vervaardigen van preparaten voor een cryo-elektronenmicroscoop. |
| DE50114369D1 (de) | 2001-06-15 | 2008-11-13 | Leica Mikrosysteme Gmbh | Verfahren und Vorrichtung zum Präparieren von Monolayern aus Zellen |
| JP2006507540A (ja) * | 2002-11-21 | 2006-03-02 | トランスフォーム・ファーマシューティカルズ・インコーポレイテッド | 凍結乾燥顕微鏡ステージ装置及びそれを用いた方法 |
| WO2004082830A2 (en) * | 2003-03-13 | 2004-09-30 | Ascend Instruments, Llc | Sample manipulation system |
| AT506233B1 (de) * | 2008-01-18 | 2009-07-15 | Leica Mikrosysteme Gmbh | Mikromanipulator für ein kryomikrotom |
| AT507079B1 (de) * | 2009-01-22 | 2010-02-15 | Leica Mikrosysteme Gmbh | Vorrichtung und verfahren zum präparieren von proben |
-
2009
- 2009-07-22 AT AT0114409A patent/AT508017B1/de not_active IP Right Cessation
-
2010
- 2010-05-22 DE DE102010021312A patent/DE102010021312B4/de not_active Expired - Fee Related
- 2010-07-20 NL NL2005116A patent/NL2005116C2/nl not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NL2005116A (nl) | 2011-01-25 |
| DE102010021312A1 (de) | 2011-03-03 |
| AT508017A4 (de) | 2010-10-15 |
| DE102010021312B4 (de) | 2013-10-24 |
| AT508017B1 (de) | 2010-10-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| V1 | Lapsed because of non-payment of the annual fee |
Effective date: 20150201 |