MXPA05014238A - Metodo para la deteccion de citosina alquilada en adn. - Google Patents

Metodo para la deteccion de citosina alquilada en adn.

Info

Publication number
MXPA05014238A
MXPA05014238A MXPA05014238A MXPA05014238A MXPA05014238A MX PA05014238 A MXPA05014238 A MX PA05014238A MX PA05014238 A MXPA05014238 A MX PA05014238A MX PA05014238 A MXPA05014238 A MX PA05014238A MX PA05014238 A MXPA05014238 A MX PA05014238A
Authority
MX
Mexico
Prior art keywords
dna
cytosine
alkylated
detection
alkylated cytosine
Prior art date
Application number
MXPA05014238A
Other languages
English (en)
Inventor
Tanya Lynn Applegate
Original Assignee
Johnson & Johnson Res Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2003903430A external-priority patent/AU2003903430A0/en
Application filed by Johnson & Johnson Res Pty Ltd filed Critical Johnson & Johnson Res Pty Ltd
Publication of MXPA05014238A publication Critical patent/MXPA05014238A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Se describe un metodo para detectar citosina alquilada en ADN de doble hebra empleando una o mas enzimas que diferencialmente modifican la citosina alquilada y la citosina. Por lo menos una region de ADN se convierte en ADN de hebra sencilla y la enzima reacciona con una region objetivo en el ADN de hebra sencilla. La presencia o nivel de citosina alquilada en la region objetivo se detecta al determinar el nivel de modificacion enzimatica de la region objetivo por la enzima.
MXPA05014238A 2003-07-04 2004-07-05 Metodo para la deteccion de citosina alquilada en adn. MXPA05014238A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2003903430A AU2003903430A0 (en) 2003-07-04 2003-07-04 Method for detection of alkylated cytosine in dna
US49199503P 2003-08-04 2003-08-04
PCT/AU2004/000900 WO2005003381A1 (en) 2003-07-04 2004-07-05 Method for detection of alkylated cytosine in dna

Publications (1)

Publication Number Publication Date
MXPA05014238A true MXPA05014238A (es) 2006-03-09

Family

ID=33565644

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA05014238A MXPA05014238A (es) 2003-07-04 2004-07-05 Metodo para la deteccion de citosina alquilada en adn.

Country Status (8)

Country Link
EP (1) EP1644519B1 (es)
KR (1) KR20060029276A (es)
AT (1) ATE419392T1 (es)
CA (1) CA2530506A1 (es)
DE (1) DE602004018776D1 (es)
IL (1) IL172910A0 (es)
MX (1) MXPA05014238A (es)
WO (1) WO2005003381A1 (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009101079A1 (en) * 2008-02-15 2009-08-20 Basf Se Pyrimidylmethyl sulfonamide compounds
US20120252015A1 (en) * 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
ES2346503B1 (es) 2008-11-03 2011-08-03 Fina Biotech, S.L.U. Metodo de diagnostico de cancer colorectal.
US8053189B2 (en) 2008-11-03 2011-11-08 Fina Biotech, S.L.U. Method for the diagnosis of colorectal cancer
JP6081911B2 (ja) 2010-06-14 2017-02-15 リケラ バイオメッド エスエーＬｙｋｅｒａ Ｂｉｏｍｅｄ Ｓａ Ｓ１００ａ４抗体およびその治療上の使用
EP2673380B1 (en) 2011-02-09 2018-12-12 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
GB201115095D0 (en) 2011-09-01 2011-10-19 Singapore Volition Pte Ltd Method for detecting nucleosomes containing nucleotides
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
ES2694131T3 (es) 2013-04-19 2018-12-18 Bayer Cropscience Nv Plantas de Brassica híbridas y métodos para producir las mismas
US10674982B2 (en) 2015-08-06 2020-06-09 Covidien Lp System and method for local three dimensional volume reconstruction using a standard fluoroscope
US10702226B2 (en) 2015-08-06 2020-07-07 Covidien Lp System and method for local three dimensional volume reconstruction using a standard fluoroscope
US10716525B2 (en) 2015-08-06 2020-07-21 Covidien Lp System and method for navigating to target and performing procedure on target utilizing fluoroscopic-based local three dimensional volume reconstruction
US11529190B2 (en) 2017-01-30 2022-12-20 Covidien Lp Enhanced ablation and visualization techniques for percutaneous surgical procedures
US11793579B2 (en) 2017-02-22 2023-10-24 Covidien Lp Integration of multiple data sources for localization and navigation
US10699448B2 (en) 2017-06-29 2020-06-30 Covidien Lp System and method for identifying, marking and navigating to a target using real time two dimensional fluoroscopic data
US10893843B2 (en) 2017-10-10 2021-01-19 Covidien Lp System and method for identifying and marking a target in a fluoroscopic three-dimensional reconstruction
US20190119746A1 (en) * 2017-10-20 2019-04-25 Nugen Technologies, Inc. Oligonucleotides for selective amplification of nucleic acids
US10893842B2 (en) 2018-02-08 2021-01-19 Covidien Lp System and method for pose estimation of an imaging device and for determining the location of a medical device with respect to a target
US11705238B2 (en) 2018-07-26 2023-07-18 Covidien Lp Systems and methods for providing assistance during surgery
EP3856903A4 (en) 2018-09-27 2022-07-27 Grail, LLC METHYLATION MARKER AND TARGETED METHYLATION PROBE PANEL
US11877806B2 (en) 2018-12-06 2024-01-23 Covidien Lp Deformable registration of computer-generated airway models to airway trees
US11801113B2 (en) 2018-12-13 2023-10-31 Covidien Lp Thoracic imaging, distance measuring, and notification system and method
US11617493B2 (en) 2018-12-13 2023-04-04 Covidien Lp Thoracic imaging, distance measuring, surgical awareness, and notification system and method
US11357593B2 (en) 2019-01-10 2022-06-14 Covidien Lp Endoscopic imaging with augmented parallax
US11625825B2 (en) 2019-01-30 2023-04-11 Covidien Lp Method for displaying tumor location within endoscopic images
US11744643B2 (en) 2019-02-04 2023-09-05 Covidien Lp Systems and methods facilitating pre-operative prediction of post-operative tissue function
US11627924B2 (en) 2019-09-24 2023-04-18 Covidien Lp Systems and methods for image-guided navigation of percutaneously-inserted devices

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10104938B4 (de) * 2001-01-29 2005-06-23 Epigenomics Ag Fluoreszenzpolarisation 1
DE10154318A1 (de) * 2001-10-26 2003-05-15 Epigenomics Ag Verfahren zur Analyse genomischer Methylierungsmuster
DE10331107B3 (de) * 2003-07-04 2004-12-02 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA mittels Cytidin-Deaminasen

Also Published As

Publication number Publication date
ATE419392T1 (de) 2009-01-15
EP1644519B1 (en) 2008-12-31
CA2530506A1 (en) 2005-01-13
DE602004018776D1 (de) 2009-02-12
EP1644519A4 (en) 2007-05-30
EP1644519A1 (en) 2006-04-12
IL172910A0 (en) 2006-06-11
WO2005003381A1 (en) 2005-01-13
KR20060029276A (ko) 2006-04-05

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