DE60325475D1 - Homogenes assay zum nachweis von enzymaktivität - Google Patents

Homogenes assay zum nachweis von enzymaktivität

Info

Publication number
DE60325475D1
DE60325475D1 DE60325475T DE60325475T DE60325475D1 DE 60325475 D1 DE60325475 D1 DE 60325475D1 DE 60325475 T DE60325475 T DE 60325475T DE 60325475 T DE60325475 T DE 60325475T DE 60325475 D1 DE60325475 D1 DE 60325475D1
Authority
DE
Germany
Prior art keywords
enzyme activity
homogeneous assay
detect enzyme
assay
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60325475T
Other languages
English (en)
Inventor
Dean M Savage
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pierce Biotechnology Inc
Original Assignee
Pierce Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pierce Biotechnology Inc filed Critical Pierce Biotechnology Inc
Application granted granted Critical
Publication of DE60325475D1 publication Critical patent/DE60325475D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE60325475T 2002-06-14 2003-06-16 Homogenes assay zum nachweis von enzymaktivität Expired - Lifetime DE60325475D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38879202P 2002-06-14 2002-06-14
PCT/US2003/018912 WO2003106703A2 (en) 2002-06-14 2003-06-16 Homogenous assay for enzymatic activity

Publications (1)

Publication Number Publication Date
DE60325475D1 true DE60325475D1 (de) 2009-02-05

Family

ID=29736542

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60325475T Expired - Lifetime DE60325475D1 (de) 2002-06-14 2003-06-16 Homogenes assay zum nachweis von enzymaktivität

Country Status (6)

Country Link
US (2) US7306928B2 (de)
EP (1) EP1516062B1 (de)
AT (1) ATE418619T1 (de)
AU (1) AU2003245513A1 (de)
DE (1) DE60325475D1 (de)
WO (1) WO2003106703A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060211075A1 (en) * 2005-03-02 2006-09-21 The Albert Einstein College Of Medicine Of Yeshiva University Enzyme sensors including environmentally sensitive or fluorescent labels and uses thereof
US8227621B2 (en) 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
JP5350800B2 (ja) * 2005-12-06 2013-11-27 プロメガ コーポレイション 環状ヌクレオチド決定のための方法
EP1991282B1 (de) 2006-02-09 2013-06-19 BioMimetic Therapeutics, LLC Zusammensetzungen und verfahren zur behandlung von knochen
US9161967B2 (en) 2006-06-30 2015-10-20 Biomimetic Therapeutics, Llc Compositions and methods for treating the vertebral column
AU2008218763B2 (en) * 2007-02-20 2013-10-24 Biomimetic Therapeutics, Llc. Prevention and treatment for osteonecrosis and osteoradionecrosis of the jaw using PDGF and a bone matrix
FI20070171A0 (fi) * 2007-02-28 2007-02-28 Hidex Oy Biotesti ja biotestivälineet
GB0712109D0 (en) * 2007-06-22 2007-08-01 Edinburgh Instr Fluorescence lifetime and fluorescence assays
US20110165603A1 (en) * 2008-08-26 2011-07-07 Gyrasol Technologies Inc. Small molecule fluorescent sensors for detection of post-translationalmodifications and protein interactions in bioassays
MX2011006586A (es) * 2008-12-19 2011-06-30 Biomimetic Therapeutics Inc Injertos de hueso con actividad de proteasa reducida y metodos de seleccion y uso.

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4568649A (en) * 1983-02-22 1986-02-04 Immunex Corporation Immediate ligand detection assay
US5034189A (en) * 1985-08-27 1991-07-23 The Regents Of The University Of California Fluorescent probe for rapid measurement of analyte concentration
EP0650396B1 (de) * 1993-05-17 1998-01-14 AMERSHAM INTERNATIONAL plc Vorrichtung und verfahren zum nachweis zellularer und biochemischer prozesse
US5538858A (en) * 1994-04-08 1996-07-23 Pierce Chemical Company Rapid assay for radioactive determination of protein kinase activity
US5527688A (en) * 1994-04-08 1996-06-18 Pierce Chemical Company Rapid assays for protein kinase activity
US7070921B2 (en) * 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US6287774B1 (en) * 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system
US6472141B2 (en) * 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
WO2001007638A2 (en) * 1999-07-27 2001-02-01 University Of Utah Research Foundation Homogeneous fluorescence method for assaying structural modifications of biomolecules

Also Published As

Publication number Publication date
EP1516062A2 (de) 2005-03-23
EP1516062B1 (de) 2008-12-24
US20080233555A1 (en) 2008-09-25
WO2003106703A3 (en) 2004-04-01
US7306928B2 (en) 2007-12-11
US20060148024A1 (en) 2006-07-06
WO2003106703A2 (en) 2003-12-24
ATE418619T1 (de) 2009-01-15
AU2003245513A1 (en) 2003-12-31
AU2003245513A8 (en) 2003-12-31

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