ATE418619T1 - Homogenes assay zum nachweis von enzymaktivität - Google Patents

Homogenes assay zum nachweis von enzymaktivität

Info

Publication number
ATE418619T1
ATE418619T1 AT03739145T AT03739145T ATE418619T1 AT E418619 T1 ATE418619 T1 AT E418619T1 AT 03739145 T AT03739145 T AT 03739145T AT 03739145 T AT03739145 T AT 03739145T AT E418619 T1 ATE418619 T1 AT E418619T1
Authority
AT
Austria
Prior art keywords
detecting
homogeneous assay
activity
enzym
assay
Prior art date
Application number
AT03739145T
Other languages
English (en)
Inventor
Dean Savage
Original Assignee
Pierce Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pierce Biotechnology Inc filed Critical Pierce Biotechnology Inc
Application granted granted Critical
Publication of ATE418619T1 publication Critical patent/ATE418619T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT03739145T 2002-06-14 2003-06-16 Homogenes assay zum nachweis von enzymaktivität ATE418619T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US38879202P 2002-06-14 2002-06-14

Publications (1)

Publication Number Publication Date
ATE418619T1 true ATE418619T1 (de) 2009-01-15

Family

ID=29736542

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03739145T ATE418619T1 (de) 2002-06-14 2003-06-16 Homogenes assay zum nachweis von enzymaktivität

Country Status (6)

Country Link
US (2) US7306928B2 (de)
EP (1) EP1516062B1 (de)
AT (1) ATE418619T1 (de)
AU (1) AU2003245513A1 (de)
DE (1) DE60325475D1 (de)
WO (1) WO2003106703A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060211075A1 (en) * 2005-03-02 2006-09-21 The Albert Einstein College Of Medicine Of Yeshiva University Enzyme sensors including environmentally sensitive or fluorescent labels and uses thereof
US8227621B2 (en) 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
CN101365721A (zh) * 2005-12-06 2009-02-11 普罗梅格公司 环核苷酸测定方法
CA2641860C (en) 2006-02-09 2015-07-14 Biomimetic Therapeutics, Inc. Compositions and methods for treating bone
US9161967B2 (en) 2006-06-30 2015-10-20 Biomimetic Therapeutics, Llc Compositions and methods for treating the vertebral column
CA2678875A1 (en) * 2007-02-20 2008-08-28 Biomimetic Therapeutics, Inc. Prevention and treatment for osteonecrosis and osteoradionecrosis of the jaw
FI20070171A0 (fi) * 2007-02-28 2007-02-28 Hidex Oy Biotesti ja biotestivälineet
GB0712109D0 (en) * 2007-06-22 2007-08-01 Edinburgh Instr Fluorescence lifetime and fluorescence assays
US20110165603A1 (en) * 2008-08-26 2011-07-07 Gyrasol Technologies Inc. Small molecule fluorescent sensors for detection of post-translationalmodifications and protein interactions in bioassays
CN102316890A (zh) * 2008-12-19 2012-01-11 生物模拟治疗公司 具有降低的蛋白酶活性的骨移植物以及选择和使用的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4568649A (en) * 1983-02-22 1986-02-04 Immunex Corporation Immediate ligand detection assay
US5034189A (en) * 1985-08-27 1991-07-23 The Regents Of The University Of California Fluorescent probe for rapid measurement of analyte concentration
ATE162216T1 (de) * 1993-05-17 1998-01-15 Amersham Int Plc Vorrichtung und verfahren zum nachweis zellularer und biochemischer prozesse
US5527688A (en) * 1994-04-08 1996-06-18 Pierce Chemical Company Rapid assays for protein kinase activity
US5538858A (en) * 1994-04-08 1996-07-23 Pierce Chemical Company Rapid assay for radioactive determination of protein kinase activity
US7070921B2 (en) * 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US6287774B1 (en) * 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system
US6472141B2 (en) * 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
EP1206699A4 (de) * 1999-07-27 2005-01-19 Univ Utah Res Found Homogenes fluoreszenzverfahren zum bestimmen struktureller modifikationen von biomolekülen

Also Published As

Publication number Publication date
DE60325475D1 (de) 2009-02-05
EP1516062A2 (de) 2005-03-23
WO2003106703A3 (en) 2004-04-01
US20060148024A1 (en) 2006-07-06
AU2003245513A8 (en) 2003-12-31
AU2003245513A1 (en) 2003-12-31
US20080233555A1 (en) 2008-09-25
US7306928B2 (en) 2007-12-11
EP1516062B1 (de) 2008-12-24
WO2003106703A2 (en) 2003-12-24

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