MXPA04003981A - Ensayo de polarizacion por fluorescencia a base de peptido para deteccion de anticuerpos para mycobacterium bovis. - Google Patents
Ensayo de polarizacion por fluorescencia a base de peptido para deteccion de anticuerpos para mycobacterium bovis.Info
- Publication number
- MXPA04003981A MXPA04003981A MXPA04003981A MXPA04003981A MXPA04003981A MX PA04003981 A MXPA04003981 A MX PA04003981A MX PA04003981 A MXPA04003981 A MX PA04003981A MX PA04003981 A MXPA04003981 A MX PA04003981A MX PA04003981 A MXPA04003981 A MX PA04003981A
- Authority
- MX
- Mexico
- Prior art keywords
- fluorescence polarization
- peptide
- bovis
- detection
- tracer
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/5695—Mycobacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Making Paper Articles (AREA)
Abstract
La presente invencion proporciona un arreglo para la deteccion de animales infectados por M. bovis. Un trazador, que comprende un peptido de la proteina MPB70 de M. bovis conjugada con un fluoroforo, es agregado a una muestra de suero de un animal para formar una mezcla. La polarizacion por fluorescencia de la mezcla luego es medida y comparada con la polarizacion por fluorescencia de un control. La presente invencion proporciona ademas un trazador para su uso en el ensayo de polarizacion por fluorescencia para detectar anticuerpos especificos para M. bovis. El trazador comprende un peptido de proteina MPB70 de M. bovis conjugada con un fluoroforo, de tal modo que el trazador sea capaz de aglutinarse a los anticuerpos especificos para M. bovis para producir un cambio detectable en la polarizacion por fluorescencia.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US33536801P | 2001-10-31 | 2001-10-31 | |
| PCT/US2002/034964 WO2003038402A2 (en) | 2001-10-31 | 2002-10-31 | A peptide-based fluorescence polarization assay for detection of antibodies to mycobacterium bovis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MXPA04003981A true MXPA04003981A (es) | 2004-10-29 |
Family
ID=23311479
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MXPA04003981A MXPA04003981A (es) | 2001-10-31 | 2002-10-31 | Ensayo de polarizacion por fluorescencia a base de peptido para deteccion de anticuerpos para mycobacterium bovis. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US7547506B2 (es) |
| EP (1) | EP1461078B1 (es) |
| KR (1) | KR100646058B1 (es) |
| CN (1) | CN1279977C (es) |
| AT (1) | ATE537840T1 (es) |
| AU (1) | AU2002363141B2 (es) |
| BR (1) | BRPI0213701B1 (es) |
| CA (1) | CA2464713C (es) |
| ES (1) | ES2379774T3 (es) |
| MX (1) | MXPA04003981A (es) |
| NZ (1) | NZ533170A (es) |
| PT (1) | PT1461078E (es) |
| WO (1) | WO2003038402A2 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3635126B1 (en) * | 2017-06-07 | 2021-07-07 | Original G B.V. | Method for detecting food spoilage microbes |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5693500A (en) * | 1988-03-31 | 1997-12-02 | Commonwealth Scientific And Industrial Research Organisation | Diagnosis of Mycobacterium bovis infection |
| ES2098511T3 (es) * | 1991-03-26 | 1997-05-01 | Dade Int Inc | Procedimiento de diagnosis de hepatitis no-a no-b. |
| US5976820A (en) * | 1995-08-28 | 1999-11-02 | Jolley; Michael E. | Detection of antibodies to bacterial antigens by flourescence polarization |
| US6110750A (en) * | 1996-06-28 | 2000-08-29 | Sugden; Edward A. | Rapid detection method of Mycobacterium bovis by fluorescence polarization |
| US6350574B1 (en) * | 1998-09-23 | 2002-02-26 | Ronald C. Montelaro | Fluorescence polarization—based diagnostic assay for equine infectious anemia virus |
-
2002
- 2002-10-31 CA CA2464713A patent/CA2464713C/en not_active Expired - Lifetime
- 2002-10-31 US US10/492,998 patent/US7547506B2/en active Active
- 2002-10-31 AU AU2002363141A patent/AU2002363141B2/en not_active Expired
- 2002-10-31 ES ES02802505T patent/ES2379774T3/es not_active Expired - Lifetime
- 2002-10-31 PT PT02802505T patent/PT1461078E/pt unknown
- 2002-10-31 MX MXPA04003981A patent/MXPA04003981A/es active IP Right Grant
- 2002-10-31 NZ NZ533170A patent/NZ533170A/en not_active IP Right Cessation
- 2002-10-31 BR BRPI0213701A patent/BRPI0213701B1/pt active IP Right Grant
- 2002-10-31 CN CNB028216415A patent/CN1279977C/zh not_active Expired - Lifetime
- 2002-10-31 EP EP02802505A patent/EP1461078B1/en not_active Expired - Lifetime
- 2002-10-31 WO PCT/US2002/034964 patent/WO2003038402A2/en not_active Application Discontinuation
- 2002-10-31 AT AT02802505T patent/ATE537840T1/de active
- 2002-10-31 KR KR1020047006581A patent/KR100646058B1/ko active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| CA2464713A1 (en) | 2003-05-08 |
| KR20050042222A (ko) | 2005-05-06 |
| AU2002363141B2 (en) | 2006-06-29 |
| WO2003038402A2 (en) | 2003-05-08 |
| EP1461078A2 (en) | 2004-09-29 |
| CA2464713C (en) | 2011-03-22 |
| EP1461078A4 (en) | 2005-08-24 |
| ES2379774T3 (es) | 2012-05-03 |
| WO2003038402A3 (en) | 2003-07-10 |
| US7547506B2 (en) | 2009-06-16 |
| BR0213701A (pt) | 2005-06-28 |
| CN1578671A (zh) | 2005-02-09 |
| US20040214250A1 (en) | 2004-10-28 |
| NZ533170A (en) | 2007-01-26 |
| PT1461078E (pt) | 2012-03-27 |
| KR100646058B1 (ko) | 2006-11-14 |
| ATE537840T1 (de) | 2012-01-15 |
| BRPI0213701B1 (pt) | 2015-12-01 |
| EP1461078B1 (en) | 2011-12-21 |
| CN1279977C (zh) | 2006-10-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |