MXPA04003981A - A peptide-based fluorescence polarization assay for detection of antibodies to mycobacterium bovis. - Google Patents

A peptide-based fluorescence polarization assay for detection of antibodies to mycobacterium bovis.

Info

Publication number
MXPA04003981A
MXPA04003981A MXPA04003981A MXPA04003981A MXPA04003981A MX PA04003981 A MXPA04003981 A MX PA04003981A MX PA04003981 A MXPA04003981 A MX PA04003981A MX PA04003981 A MXPA04003981 A MX PA04003981A MX PA04003981 A MXPA04003981 A MX PA04003981A
Authority
MX
Mexico
Prior art keywords
fluorescence polarization
peptide
bovis
detection
tracer
Prior art date
Application number
MXPA04003981A
Other languages
Spanish (es)
Inventor
Sarwar Nasir Mohammad
Original Assignee
Diachemix Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diachemix Llc filed Critical Diachemix Llc
Publication of MXPA04003981A publication Critical patent/MXPA04003981A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/5695—Mycobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

The present invention provides an assay for detection of M. bovis-infected animals. A tracer, comprising a peptide of M. bovis protein MPB70 conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture in then measured and compared to the fluorescence polarization of a control. The present invention further provides a tracer for use in fluorescence polarization assay to detect antibodies specific for M. bovis. The tracer comprises a peptide of M. bovis protein MPB70 conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for M. bovis to produce a detectable change in fluorescence polarization.
MXPA04003981A 2001-10-31 2002-10-31 A peptide-based fluorescence polarization assay for detection of antibodies to mycobacterium bovis. MXPA04003981A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33536801P 2001-10-31 2001-10-31
PCT/US2002/034964 WO2003038402A2 (en) 2001-10-31 2002-10-31 A peptide-based fluorescence polarization assay for detection of antibodies to mycobacterium bovis

Publications (1)

Publication Number Publication Date
MXPA04003981A true MXPA04003981A (en) 2004-10-29

Family

ID=23311479

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA04003981A MXPA04003981A (en) 2001-10-31 2002-10-31 A peptide-based fluorescence polarization assay for detection of antibodies to mycobacterium bovis.

Country Status (13)

Country Link
US (1) US7547506B2 (en)
EP (1) EP1461078B1 (en)
KR (1) KR100646058B1 (en)
CN (1) CN1279977C (en)
AT (1) ATE537840T1 (en)
AU (1) AU2002363141B2 (en)
BR (1) BRPI0213701B1 (en)
CA (1) CA2464713C (en)
ES (1) ES2379774T3 (en)
MX (1) MXPA04003981A (en)
NZ (1) NZ533170A (en)
PT (1) PT1461078E (en)
WO (1) WO2003038402A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2886852T3 (en) * 2017-06-07 2021-12-21 Original G B V Method for detecting food spoilage microbes

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693500A (en) * 1988-03-31 1997-12-02 Commonwealth Scientific And Industrial Research Organisation Diagnosis of Mycobacterium bovis infection
DE69216333T2 (en) * 1991-03-26 1997-07-10 Dade Int Inc NOT-A, NOT-B HEPATITIS TEST
US5976820A (en) * 1995-08-28 1999-11-02 Jolley; Michael E. Detection of antibodies to bacterial antigens by flourescence polarization
US6110750A (en) * 1996-06-28 2000-08-29 Sugden; Edward A. Rapid detection method of Mycobacterium bovis by fluorescence polarization
US6350574B1 (en) * 1998-09-23 2002-02-26 Ronald C. Montelaro Fluorescence polarization—based diagnostic assay for equine infectious anemia virus

Also Published As

Publication number Publication date
CN1279977C (en) 2006-10-18
KR100646058B1 (en) 2006-11-14
NZ533170A (en) 2007-01-26
PT1461078E (en) 2012-03-27
CA2464713A1 (en) 2003-05-08
CN1578671A (en) 2005-02-09
WO2003038402A3 (en) 2003-07-10
KR20050042222A (en) 2005-05-06
AU2002363141B2 (en) 2006-06-29
US7547506B2 (en) 2009-06-16
EP1461078B1 (en) 2011-12-21
WO2003038402A2 (en) 2003-05-08
ES2379774T3 (en) 2012-05-03
ATE537840T1 (en) 2012-01-15
CA2464713C (en) 2011-03-22
BRPI0213701B1 (en) 2015-12-01
BR0213701A (en) 2005-06-28
US20040214250A1 (en) 2004-10-28
EP1461078A2 (en) 2004-09-29
EP1461078A4 (en) 2005-08-24

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