MXPA04003980A - Prueba serologica rapida para paratuberculosis utilizando tecnologia de polarizacion por fluorescencia. - Google Patents
Prueba serologica rapida para paratuberculosis utilizando tecnologia de polarizacion por fluorescencia.Info
- Publication number
- MXPA04003980A MXPA04003980A MXPA04003980A MXPA04003980A MXPA04003980A MX PA04003980 A MXPA04003980 A MX PA04003980A MX PA04003980 A MXPA04003980 A MX PA04003980A MX PA04003980 A MXPA04003980 A MX PA04003980A MX PA04003980 A MXPA04003980 A MX PA04003980A
- Authority
- MX
- Mexico
- Prior art keywords
- paratuberculosis
- fluorescence polarization
- tracer
- mixture
- fluorophore
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/5695—Mycobacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/345—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Brevibacterium (G)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invencion proporciona un ensayo para la deteccion de anticuerpos del suero para M. paratuberculosis. Un trazador, que comprende un antigeno de carbohidrato aislado de M. paratuberculosis que esta conjugado con un fluoroforo, es agregado a una muestra del suero de un animal para formar una mezcla La polarizacion por fluorescencia de la mezcla es medida entonces. La presencia de los anticuerpos del suero para M. paratubercolisis esta indicada por un valor de polarizacion por fluorescencia de la mezcla que es mas elevado que el valor de polarizacion por fluorescencia de un control. La presente invencion proporciona ademas un trazador para su uso en un ensayo de polarizacion por fluorescencia para anticuerpos especificos para M. paratuberculosis. El trazador comprende un antigeno de carbohidrato aislado de M. paratuberculosis y conjugado con un fluoroforo, de tal modo que el trazador sea capaz de aglutinarse a los anticuerpos especificos para M. paratuberculosis para producir un cambio detectable en la polarizacion por fluorescencia.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33525301P | 2001-10-31 | 2001-10-31 | |
PCT/US2002/035002 WO2003037369A1 (en) | 2001-10-31 | 2002-10-31 | A rapid serological test for paratuberculosis using fluorescence polarization technology |
Publications (1)
Publication Number | Publication Date |
---|---|
MXPA04003980A true MXPA04003980A (es) | 2004-11-29 |
Family
ID=23310950
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MXPA04003980A MXPA04003980A (es) | 2001-10-31 | 2002-10-31 | Prueba serologica rapida para paratuberculosis utilizando tecnologia de polarizacion por fluorescencia. |
Country Status (10)
Country | Link |
---|---|
US (1) | US7018616B2 (es) |
EP (1) | EP1461075A4 (es) |
KR (1) | KR20050042042A (es) |
CN (1) | CN1777441A (es) |
AU (1) | AU2002336705B2 (es) |
BR (1) | BR0213700A (es) |
CA (1) | CA2464473A1 (es) |
MX (1) | MXPA04003980A (es) |
NZ (1) | NZ533171A (es) |
WO (1) | WO2003037369A1 (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102262077A (zh) * | 2010-05-26 | 2011-11-30 | 电子科技大学 | 一种锐化的癌症早期诊断和治疗效果检查的装置 |
CN114166807B (zh) * | 2021-11-22 | 2023-12-12 | 中国农业大学 | 用于检测多粘菌素的荧光偏振免疫分析方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6387372B1 (en) | 1991-03-25 | 2002-05-14 | N.V. Innogenetics S.A. | Polypeptides from mycrobacterium paratuberculosis |
US5976820A (en) | 1995-08-28 | 1999-11-02 | Jolley; Michael E. | Detection of antibodies to bacterial antigens by flourescence polarization |
US6110750A (en) | 1996-06-28 | 2000-08-29 | Sugden; Edward A. | Rapid detection method of Mycobacterium bovis by fluorescence polarization |
-
2002
- 2002-10-31 WO PCT/US2002/035002 patent/WO2003037369A1/en not_active Application Discontinuation
- 2002-10-31 MX MXPA04003980A patent/MXPA04003980A/es not_active Application Discontinuation
- 2002-10-31 AU AU2002336705A patent/AU2002336705B2/en not_active Ceased
- 2002-10-31 EP EP02773957A patent/EP1461075A4/en not_active Withdrawn
- 2002-10-31 CA CA002464473A patent/CA2464473A1/en not_active Abandoned
- 2002-10-31 US US10/493,095 patent/US7018616B2/en not_active Expired - Lifetime
- 2002-10-31 KR KR1020047006582A patent/KR20050042042A/ko not_active Application Discontinuation
- 2002-10-31 CN CN02821533.8A patent/CN1777441A/zh active Pending
- 2002-10-31 NZ NZ533171A patent/NZ533171A/en not_active IP Right Cessation
- 2002-10-31 BR BRPI0213700-3A patent/BR0213700A/pt not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
US20040191795A1 (en) | 2004-09-30 |
NZ533171A (en) | 2006-08-31 |
WO2003037369A1 (en) | 2003-05-08 |
EP1461075A1 (en) | 2004-09-29 |
CA2464473A1 (en) | 2003-05-08 |
EP1461075A4 (en) | 2006-01-25 |
BR0213700A (pt) | 2006-05-23 |
KR20050042042A (ko) | 2005-05-04 |
CN1777441A (zh) | 2006-05-24 |
AU2002336705B2 (en) | 2006-06-15 |
US7018616B2 (en) | 2006-03-28 |
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Legal Events
Date | Code | Title | Description |
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FA | Abandonment or withdrawal |