MXPA04003980A - Prueba serologica rapida para paratuberculosis utilizando tecnologia de polarizacion por fluorescencia. - Google Patents

Prueba serologica rapida para paratuberculosis utilizando tecnologia de polarizacion por fluorescencia.

Info

Publication number
MXPA04003980A
MXPA04003980A MXPA04003980A MXPA04003980A MXPA04003980A MX PA04003980 A MXPA04003980 A MX PA04003980A MX PA04003980 A MXPA04003980 A MX PA04003980A MX PA04003980 A MXPA04003980 A MX PA04003980A MX PA04003980 A MXPA04003980 A MX PA04003980A
Authority
MX
Mexico
Prior art keywords
paratuberculosis
fluorescence polarization
tracer
mixture
fluorophore
Prior art date
Application number
MXPA04003980A
Other languages
English (en)
Inventor
Irene Stilwell Kathryn
Original Assignee
Diachemix Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diachemix Llc filed Critical Diachemix Llc
Publication of MXPA04003980A publication Critical patent/MXPA04003980A/es

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911Bacteria
    • G01N33/5695Mycobacteria
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/345Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Brevibacterium (G)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00Immunoassays for the detection of microorganisms
    • G01N2469/20Detection of antibodies in sample from host which are directed against antigens from microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invencion proporciona un ensayo para la deteccion de anticuerpos del suero para M. paratuberculosis. Un trazador, que comprende un antigeno de carbohidrato aislado de M. paratuberculosis que esta conjugado con un fluoroforo, es agregado a una muestra del suero de un animal para formar una mezcla La polarizacion por fluorescencia de la mezcla es medida entonces. La presencia de los anticuerpos del suero para M. paratubercolisis esta indicada por un valor de polarizacion por fluorescencia de la mezcla que es mas elevado que el valor de polarizacion por fluorescencia de un control. La presente invencion proporciona ademas un trazador para su uso en un ensayo de polarizacion por fluorescencia para anticuerpos especificos para M. paratuberculosis. El trazador comprende un antigeno de carbohidrato aislado de M. paratuberculosis y conjugado con un fluoroforo, de tal modo que el trazador sea capaz de aglutinarse a los anticuerpos especificos para M. paratuberculosis para producir un cambio detectable en la polarizacion por fluorescencia.
MXPA04003980A 2001-10-31 2002-10-31 Prueba serologica rapida para paratuberculosis utilizando tecnologia de polarizacion por fluorescencia. MXPA04003980A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33525301P 2001-10-31 2001-10-31
PCT/US2002/035002 WO2003037369A1 (en) 2001-10-31 2002-10-31 A rapid serological test for paratuberculosis using fluorescence polarization technology

Publications (1)

Publication Number Publication Date
MXPA04003980A true MXPA04003980A (es) 2004-11-29

Family

ID=23310950

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA04003980A MXPA04003980A (es) 2001-10-31 2002-10-31 Prueba serologica rapida para paratuberculosis utilizando tecnologia de polarizacion por fluorescencia.

Country Status (10)

Country Link
US (1) US7018616B2 (es)
EP (1) EP1461075A4 (es)
KR (1) KR20050042042A (es)
CN (1) CN1777441A (es)
AU (1) AU2002336705B2 (es)
BR (1) BR0213700A (es)
CA (1) CA2464473A1 (es)
MX (1) MXPA04003980A (es)
NZ (1) NZ533171A (es)
WO (1) WO2003037369A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102262077A (zh) * 2010-05-26 2011-11-30 电子科技大学 一种锐化的癌症早期诊断和治疗效果检查的装置
CN114166807B (zh) * 2021-11-22 2023-12-12 中国农业大学 用于检测多粘菌素的荧光偏振免疫分析方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6387372B1 (en) 1991-03-25 2002-05-14 N.V. Innogenetics S.A. Polypeptides from mycrobacterium paratuberculosis
US5976820A (en) 1995-08-28 1999-11-02 Jolley; Michael E. Detection of antibodies to bacterial antigens by flourescence polarization
US6110750A (en) 1996-06-28 2000-08-29 Sugden; Edward A. Rapid detection method of Mycobacterium bovis by fluorescence polarization

Also Published As

Publication number Publication date
US20040191795A1 (en) 2004-09-30
NZ533171A (en) 2006-08-31
WO2003037369A1 (en) 2003-05-08
EP1461075A1 (en) 2004-09-29
CA2464473A1 (en) 2003-05-08
EP1461075A4 (en) 2006-01-25
BR0213700A (pt) 2006-05-23
KR20050042042A (ko) 2005-05-04
CN1777441A (zh) 2006-05-24
AU2002336705B2 (en) 2006-06-15
US7018616B2 (en) 2006-03-28

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