BR0213701A - Método para detectar animais infectados com m. bovis, traçador para detectar anticorpos para m. bovis no ensaio de polarização da fluorescência, e, kit para detectar anticorpos para m. bovis em uma amostra tomada de um animal - Google Patents
Método para detectar animais infectados com m. bovis, traçador para detectar anticorpos para m. bovis no ensaio de polarização da fluorescência, e, kit para detectar anticorpos para m. bovis em uma amostra tomada de um animalInfo
- Publication number
- BR0213701A BR0213701A BR0213701-1A BR0213701A BR0213701A BR 0213701 A BR0213701 A BR 0213701A BR 0213701 A BR0213701 A BR 0213701A BR 0213701 A BR0213701 A BR 0213701A
- Authority
- BR
- Brazil
- Prior art keywords
- bovis
- detecting
- tracer
- fluorescence polarization
- detecting antibodies
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/5695—Mycobacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Making Paper Articles (AREA)
Abstract
"MéTODO PARA DETECTAR ANIMAIS INFECTADOS COM M. BOVIS, TRAçADOR PARA DETECTAR ANTICORPOS PARA M. BOVIS NO ENSAIO DE POLARIZAçãO DA FLUORESCêNCIA, E, KIT PARA DETECTAR ANTICORPOS PARA M. BOVIS EM UMA AMOSTRA TOMADA DE UM ANIMAL". A presente invenção provê um ensaio para a detecção de animais infectados por M. bovis. Um traçador, compreendendo um peptídeo de proteína MPB70 de M bovis, conjugado a um fluoróforo, é adicionado a uma amostra de soro de um animal para formar uma mistura. A polarização da fluorescência da mistura é então medida e comparada com a polarização da fluorescência de um controle. A presente invenção ainda provê um traçador para uso em ensaio de polarização da fluorescência para detectar anticorpos específicos para M. bovis. O traçador compreende um peptídeo de proteina MPB70 de M bovis conjugado a um fluoróforo, de modo que o traçador é capaz de ligar a anticorpos específicos para M. bovis para produzir uma mudança detectável em polarização da fluorescência.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US33536801P | 2001-10-31 | 2001-10-31 | |
| PCT/US2002/034964 WO2003038402A2 (en) | 2001-10-31 | 2002-10-31 | A peptide-based fluorescence polarization assay for detection of antibodies to mycobacterium bovis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR0213701A true BR0213701A (pt) | 2005-06-28 |
| BRPI0213701B1 BRPI0213701B1 (pt) | 2015-12-01 |
Family
ID=23311479
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0213701A BRPI0213701B1 (pt) | 2001-10-31 | 2002-10-31 | método para detectar animais infectados com m. bovis, traçador para detectar anticorpos para m. bovis no ensaio de polarização da fluorescência |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US7547506B2 (pt) |
| EP (1) | EP1461078B1 (pt) |
| KR (1) | KR100646058B1 (pt) |
| CN (1) | CN1279977C (pt) |
| AT (1) | ATE537840T1 (pt) |
| AU (1) | AU2002363141B2 (pt) |
| BR (1) | BRPI0213701B1 (pt) |
| CA (1) | CA2464713C (pt) |
| ES (1) | ES2379774T3 (pt) |
| MX (1) | MXPA04003981A (pt) |
| NZ (1) | NZ533170A (pt) |
| PT (1) | PT1461078E (pt) |
| WO (1) | WO2003038402A2 (pt) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3635126B1 (en) * | 2017-06-07 | 2021-07-07 | Original G B.V. | Method for detecting food spoilage microbes |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5693500A (en) * | 1988-03-31 | 1997-12-02 | Commonwealth Scientific And Industrial Research Organisation | Diagnosis of Mycobacterium bovis infection |
| ES2098511T3 (es) * | 1991-03-26 | 1997-05-01 | Dade Int Inc | Procedimiento de diagnosis de hepatitis no-a no-b. |
| US5976820A (en) * | 1995-08-28 | 1999-11-02 | Jolley; Michael E. | Detection of antibodies to bacterial antigens by flourescence polarization |
| US6110750A (en) * | 1996-06-28 | 2000-08-29 | Sugden; Edward A. | Rapid detection method of Mycobacterium bovis by fluorescence polarization |
| US6350574B1 (en) * | 1998-09-23 | 2002-02-26 | Ronald C. Montelaro | Fluorescence polarization—based diagnostic assay for equine infectious anemia virus |
-
2002
- 2002-10-31 CA CA2464713A patent/CA2464713C/en not_active Expired - Lifetime
- 2002-10-31 US US10/492,998 patent/US7547506B2/en active Active
- 2002-10-31 AU AU2002363141A patent/AU2002363141B2/en not_active Expired
- 2002-10-31 ES ES02802505T patent/ES2379774T3/es not_active Expired - Lifetime
- 2002-10-31 PT PT02802505T patent/PT1461078E/pt unknown
- 2002-10-31 MX MXPA04003981A patent/MXPA04003981A/es active IP Right Grant
- 2002-10-31 NZ NZ533170A patent/NZ533170A/en not_active IP Right Cessation
- 2002-10-31 BR BRPI0213701A patent/BRPI0213701B1/pt active IP Right Grant
- 2002-10-31 CN CNB028216415A patent/CN1279977C/zh not_active Expired - Lifetime
- 2002-10-31 EP EP02802505A patent/EP1461078B1/en not_active Expired - Lifetime
- 2002-10-31 WO PCT/US2002/034964 patent/WO2003038402A2/en not_active Application Discontinuation
- 2002-10-31 AT AT02802505T patent/ATE537840T1/de active
- 2002-10-31 KR KR1020047006581A patent/KR100646058B1/ko active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| CA2464713A1 (en) | 2003-05-08 |
| MXPA04003981A (es) | 2004-10-29 |
| KR20050042222A (ko) | 2005-05-06 |
| AU2002363141B2 (en) | 2006-06-29 |
| WO2003038402A2 (en) | 2003-05-08 |
| EP1461078A2 (en) | 2004-09-29 |
| CA2464713C (en) | 2011-03-22 |
| EP1461078A4 (en) | 2005-08-24 |
| ES2379774T3 (es) | 2012-05-03 |
| WO2003038402A3 (en) | 2003-07-10 |
| US7547506B2 (en) | 2009-06-16 |
| CN1578671A (zh) | 2005-02-09 |
| US20040214250A1 (en) | 2004-10-28 |
| NZ533170A (en) | 2007-01-26 |
| PT1461078E (pt) | 2012-03-27 |
| KR100646058B1 (ko) | 2006-11-14 |
| ATE537840T1 (de) | 2012-01-15 |
| BRPI0213701B1 (pt) | 2015-12-01 |
| EP1461078B1 (en) | 2011-12-21 |
| CN1279977C (zh) | 2006-10-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B15K | Others concerning applications: alteration of classification |
Free format text: ALTERADA DE: A61K 39/00, A61K 39/04, A61K 39/40, C12N 1/12, G01N 33/53, G01N 33/531, G01N 33/533, G01N 33/566. Ipc: G01N 33/566 (2006.01), C07K 14/35 (2006.01) |
|
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 01/12/2015, OBSERVADAS AS CONDICOES LEGAIS. |