MXPA01013031A - Metodo para determinar el volumen de celulas de sangre roja sencillas. - Google Patents
Metodo para determinar el volumen de celulas de sangre roja sencillas.Info
- Publication number
- MXPA01013031A MXPA01013031A MXPA01013031A MXPA01013031A MXPA01013031A MX PA01013031 A MXPA01013031 A MX PA01013031A MX PA01013031 A MXPA01013031 A MX PA01013031A MX PA01013031 A MXPA01013031 A MX PA01013031A MX PA01013031 A MXPA01013031 A MX PA01013031A
- Authority
- MX
- Mexico
- Prior art keywords
- volume
- determining
- red blood
- blood cells
- cells
- Prior art date
Links
- 210000003743 erythrocyte Anatomy 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 5
- 230000005284 excitation Effects 0.000 abstract 1
- 239000007850 fluorescent dye Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 238000007431 microscopic evaluation Methods 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/012—Red blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1497—Particle shape
Landscapes
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Optical Measuring Cells (AREA)
Abstract
La invencion describe un metodo para determinar el volumen de celulas de sangre roja sencillas u otras particulas que estan suspendidas en liquidos. La muestra se dispone en una cubeta optica apropiada para analisis microscopico. Se anade un tinte fluorescente que no se fuga hacia las celulas, y que es capaz de absorber luz de excitacion y emitir luz de fluorescencia a longitudes de onda que son solamente absorbidas de manera debil por las celulas. El volumen de celula se determina utilizando valores de intensidad de fluorescencia medidos (I)en una primera area que comprende una sola celula. (II) una segunda area cerca de esta celula y (III) en la segunda area despues de cambiar el espesor de cubeta en una cantidad conocida.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/753,205 US6633368B2 (en) | 2001-01-02 | 2001-01-02 | Method for determining the volume of single red blood cells |
Publications (1)
Publication Number | Publication Date |
---|---|
MXPA01013031A true MXPA01013031A (es) | 2004-06-25 |
Family
ID=25029623
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MXPA01013031A MXPA01013031A (es) | 2001-01-02 | 2001-12-17 | Metodo para determinar el volumen de celulas de sangre roja sencillas. |
Country Status (6)
Country | Link |
---|---|
US (1) | US6633368B2 (es) |
EP (1) | EP1219950B1 (es) |
JP (1) | JP2002318188A (es) |
AT (1) | ATE296442T1 (es) |
DE (1) | DE60111010T2 (es) |
MX (1) | MXPA01013031A (es) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040090613A1 (en) * | 2002-07-17 | 2004-05-13 | Goix Philippe J. | Method for measuring the volume of cells or particles |
US6873725B2 (en) * | 2002-09-09 | 2005-03-29 | Coulter International Corp. | Simultaneous measurement and display of 3-D size distributions of particulate materials in suspensions |
GB0427050D0 (en) * | 2004-12-10 | 2005-01-12 | Amersham Biosciences Uk Ltd | Method of,and apparatus and computer software for,imaging biological objects |
EP2635896A1 (en) | 2010-11-03 | 2013-09-11 | Reametrix Inc. | Method and device for fluorescent measurement of samples |
JP6010033B2 (ja) | 2011-08-26 | 2016-10-19 | オリンパス株式会社 | 光分析を用いた単一粒子検出装置、単一粒子検出方法及び単一粒子検出用コンピュータプログラム |
CN104136915B (zh) | 2012-04-18 | 2016-07-13 | 奥林巴斯株式会社 | 目标粒子的检测方法 |
WO2013157319A1 (ja) | 2012-04-18 | 2013-10-24 | オリンパス株式会社 | 光分析を用いた単一粒子検出装置、単一粒子検出方法及び単一粒子検出用コンピュータプログラム |
CN104685346B (zh) | 2012-05-02 | 2018-05-29 | 查尔斯河实验室公司 | 细胞捕获系统及其使用 |
CN104662425B (zh) | 2012-05-02 | 2017-10-10 | 查尔斯河实验室公司 | 活性染色方法 |
EP2780707B1 (en) | 2012-05-02 | 2016-02-24 | Charles River Laboratories, Inc. | Method of detecting viable cells in a cell sample |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR1471863A (es) * | 1965-03-22 | 1967-05-10 | ||
US4284355A (en) * | 1979-10-29 | 1981-08-18 | Ortho Diagnostics, Inc. | Automated method for cell volume determination |
DE69118295T2 (de) * | 1990-10-01 | 1996-09-19 | Canon Kk | Vorrichtung und Verfahren zur Messung einer Probe |
US5563070A (en) * | 1993-05-28 | 1996-10-08 | Omron Corporation | Method of counting reticulocytes |
US5948686A (en) * | 1998-03-07 | 1999-09-07 | Robert A. Leuine | Method for performing blood cell counts |
US6235536B1 (en) * | 1998-03-07 | 2001-05-22 | Robert A. Levine | Analysis of quiescent anticoagulated whole blood samples |
US6127184A (en) * | 1998-03-07 | 2000-10-03 | Robert A. Levine | Calibration of a whole blood sample analyzer |
US6359683B1 (en) * | 2000-04-27 | 2002-03-19 | Becton, Dickinson And Company | Method for determining the volume of particles suspended in liquids |
-
2001
- 2001-01-02 US US09/753,205 patent/US6633368B2/en not_active Expired - Lifetime
- 2001-12-07 DE DE60111010T patent/DE60111010T2/de not_active Expired - Fee Related
- 2001-12-07 EP EP01129068A patent/EP1219950B1/en not_active Expired - Lifetime
- 2001-12-07 AT AT01129068T patent/ATE296442T1/de not_active IP Right Cessation
- 2001-12-17 MX MXPA01013031A patent/MXPA01013031A/es unknown
-
2002
- 2002-01-04 JP JP2002000193A patent/JP2002318188A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
DE60111010T2 (de) | 2006-05-04 |
US20020126272A1 (en) | 2002-09-12 |
ATE296442T1 (de) | 2005-06-15 |
DE60111010D1 (de) | 2005-06-30 |
EP1219950B1 (en) | 2005-05-25 |
US6633368B2 (en) | 2003-10-14 |
EP1219950A1 (en) | 2002-07-03 |
JP2002318188A (ja) | 2002-10-31 |
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