MXPA01013031A - Metodo para determinar el volumen de celulas de sangre roja sencillas. - Google Patents

Metodo para determinar el volumen de celulas de sangre roja sencillas.

Info

Publication number
MXPA01013031A
MXPA01013031A MXPA01013031A MXPA01013031A MXPA01013031A MX PA01013031 A MXPA01013031 A MX PA01013031A MX PA01013031 A MXPA01013031 A MX PA01013031A MX PA01013031 A MXPA01013031 A MX PA01013031A MX PA01013031 A MXPA01013031 A MX PA01013031A
Authority
MX
Mexico
Prior art keywords
volume
determining
red blood
blood cells
cells
Prior art date
Application number
MXPA01013031A
Other languages
English (en)
Inventor
W Berndt Klaus
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Publication of MXPA01013031A publication Critical patent/MXPA01013031A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/012—Red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497—Particle shape

Landscapes

  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Optical Measuring Cells (AREA)

Abstract

La invencion describe un metodo para determinar el volumen de celulas de sangre roja sencillas u otras particulas que estan suspendidas en liquidos. La muestra se dispone en una cubeta optica apropiada para analisis microscopico. Se anade un tinte fluorescente que no se fuga hacia las celulas, y que es capaz de absorber luz de excitacion y emitir luz de fluorescencia a longitudes de onda que son solamente absorbidas de manera debil por las celulas. El volumen de celula se determina utilizando valores de intensidad de fluorescencia medidos (I)en una primera area que comprende una sola celula. (II) una segunda area cerca de esta celula y (III) en la segunda area despues de cambiar el espesor de cubeta en una cantidad conocida.
MXPA01013031A 2001-01-02 2001-12-17 Metodo para determinar el volumen de celulas de sangre roja sencillas. MXPA01013031A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/753,205 US6633368B2 (en) 2001-01-02 2001-01-02 Method for determining the volume of single red blood cells

Publications (1)

Publication Number Publication Date
MXPA01013031A true MXPA01013031A (es) 2004-06-25

Family

ID=25029623

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA01013031A MXPA01013031A (es) 2001-01-02 2001-12-17 Metodo para determinar el volumen de celulas de sangre roja sencillas.

Country Status (6)

Country Link
US (1) US6633368B2 (es)
EP (1) EP1219950B1 (es)
JP (1) JP2002318188A (es)
AT (1) ATE296442T1 (es)
DE (1) DE60111010T2 (es)
MX (1) MXPA01013031A (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040090613A1 (en) * 2002-07-17 2004-05-13 Goix Philippe J. Method for measuring the volume of cells or particles
US6873725B2 (en) * 2002-09-09 2005-03-29 Coulter International Corp. Simultaneous measurement and display of 3-D size distributions of particulate materials in suspensions
GB0427050D0 (en) * 2004-12-10 2005-01-12 Amersham Biosciences Uk Ltd Method of,and apparatus and computer software for,imaging biological objects
EP2635896A1 (en) 2010-11-03 2013-09-11 Reametrix Inc. Method and device for fluorescent measurement of samples
JP6010033B2 (ja) 2011-08-26 2016-10-19 オリンパス株式会社 光分析を用いた単一粒子検出装置、単一粒子検出方法及び単一粒子検出用コンピュータプログラム
CN104136915B (zh) 2012-04-18 2016-07-13 奥林巴斯株式会社 目标粒子的检测方法
WO2013157319A1 (ja) 2012-04-18 2013-10-24 オリンパス株式会社 光分析を用いた単一粒子検出装置、単一粒子検出方法及び単一粒子検出用コンピュータプログラム
CN104685346B (zh) 2012-05-02 2018-05-29 查尔斯河实验室公司 细胞捕获系统及其使用
CN104662425B (zh) 2012-05-02 2017-10-10 查尔斯河实验室公司 活性染色方法
EP2780707B1 (en) 2012-05-02 2016-02-24 Charles River Laboratories, Inc. Method of detecting viable cells in a cell sample

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1471863A (es) * 1965-03-22 1967-05-10
US4284355A (en) * 1979-10-29 1981-08-18 Ortho Diagnostics, Inc. Automated method for cell volume determination
DE69118295T2 (de) * 1990-10-01 1996-09-19 Canon Kk Vorrichtung und Verfahren zur Messung einer Probe
US5563070A (en) * 1993-05-28 1996-10-08 Omron Corporation Method of counting reticulocytes
US5948686A (en) * 1998-03-07 1999-09-07 Robert A. Leuine Method for performing blood cell counts
US6235536B1 (en) * 1998-03-07 2001-05-22 Robert A. Levine Analysis of quiescent anticoagulated whole blood samples
US6127184A (en) * 1998-03-07 2000-10-03 Robert A. Levine Calibration of a whole blood sample analyzer
US6359683B1 (en) * 2000-04-27 2002-03-19 Becton, Dickinson And Company Method for determining the volume of particles suspended in liquids

Also Published As

Publication number Publication date
DE60111010T2 (de) 2006-05-04
US20020126272A1 (en) 2002-09-12
ATE296442T1 (de) 2005-06-15
DE60111010D1 (de) 2005-06-30
EP1219950B1 (en) 2005-05-25
US6633368B2 (en) 2003-10-14
EP1219950A1 (en) 2002-07-03
JP2002318188A (ja) 2002-10-31

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