WO2006127590A3 - Microfluidic detection cell for stimulated radiation measurements - Google Patents

Microfluidic detection cell for stimulated radiation measurements Download PDF

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Publication number
WO2006127590A3
WO2006127590A3 PCT/US2006/019714 US2006019714W WO2006127590A3 WO 2006127590 A3 WO2006127590 A3 WO 2006127590A3 US 2006019714 W US2006019714 W US 2006019714W WO 2006127590 A3 WO2006127590 A3 WO 2006127590A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
detection cell
capillary tube
stimulated radiation
radiation measurements
Prior art date
Application number
PCT/US2006/019714
Other languages
French (fr)
Other versions
WO2006127590A2 (en
Inventor
Paul D Smith
Nicole Y Morgan
Ed Wellner
Terry M Phillips
Original Assignee
Us Gov Health & Human Serv
Paul D Smith
Nicole Y Morgan
Ed Wellner
Terry M Phillips
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv, Paul D Smith, Nicole Y Morgan, Ed Wellner, Terry M Phillips filed Critical Us Gov Health & Human Serv
Publication of WO2006127590A2 publication Critical patent/WO2006127590A2/en
Publication of WO2006127590A3 publication Critical patent/WO2006127590A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G01N2021/052—Tubular type; cavity type; multireflective
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A microf luidic detection cell (339) for stimulated radiation measurements, such as fluorescence or phosphorescence can have a detection volume of 1 μL or less and a sample can be excited using two excitation wavelengths. The detection cell can include a 5 mm to 10 cm long capillary tube (110, 340) and an excitation fiber (120, 320) proximate to the capillary tube (110, 340) . A detection fiber (150, 345) is also proximate to the capillary tube (110, 340) , and the detection fiber (150, 345) has a diameter the same size or larger than the internal diameter of the capillary tube (110, 340) . A plurality of either or both of excitation (120, 320) and detection fibers (150, 345) can be used.
PCT/US2006/019714 2005-05-20 2006-05-19 Microfluidic detection cell for stimulated radiation measurements WO2006127590A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68284705P 2005-05-20 2005-05-20
US60/682,847 2005-05-20

Publications (2)

Publication Number Publication Date
WO2006127590A2 WO2006127590A2 (en) 2006-11-30
WO2006127590A3 true WO2006127590A3 (en) 2007-01-25

Family

ID=37312030

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/019714 WO2006127590A2 (en) 2005-05-20 2006-05-19 Microfluidic detection cell for stimulated radiation measurements

Country Status (1)

Country Link
WO (1) WO2006127590A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012055432A1 (en) * 2010-10-27 2012-05-03 Agilent Technologies, Inc. Waveguide cell with enhanced numerical aperture
CN103257128B (en) * 2013-05-13 2015-10-07 上海通微分析技术有限公司 serial double light path laser induced fluorescence spectrometer

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2090768A (en) * 1981-01-08 1982-07-21 Varian Associates Method and apparatus for on-column detection in liquid chromatography
EP0587281A2 (en) * 1992-07-17 1994-03-16 Beckman Instruments, Inc. Capillary electrophoresis detection
US5903348A (en) * 1997-03-12 1999-05-11 Nz Applied Technologies, Inc. System and method for molecular sample measurements
US20030116436A1 (en) * 2001-10-19 2003-06-26 Varouj Amirkhanian Multi-color multiplexed analysis in a bio-separation system
WO2004055509A2 (en) * 2002-12-13 2004-07-01 Biocal Technology, Inc. Optical detection alignment coupling apparatus

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2090768A (en) * 1981-01-08 1982-07-21 Varian Associates Method and apparatus for on-column detection in liquid chromatography
EP0587281A2 (en) * 1992-07-17 1994-03-16 Beckman Instruments, Inc. Capillary electrophoresis detection
US5903348A (en) * 1997-03-12 1999-05-11 Nz Applied Technologies, Inc. System and method for molecular sample measurements
US20030116436A1 (en) * 2001-10-19 2003-06-26 Varouj Amirkhanian Multi-color multiplexed analysis in a bio-separation system
WO2004055509A2 (en) * 2002-12-13 2004-07-01 Biocal Technology, Inc. Optical detection alignment coupling apparatus

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AIELLO M ET AL: "A SENSITIVE SMALL-VOLUME UV/VIS FLOW CELL AND TOTAL ABSORBANCE DETECTION SYSTEM FOR MICRO-HPLC", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 73, no. 6, 15 March 2001 (2001-03-15), pages 1387 - 1392, XP001063581, ISSN: 0003-2700 *
MAJORS R. E.: "Fused-silica capillary - the story behind the technology", LCGC NORTH AMERICA, vol. 20, no. 10, 1 October 2002 (2002-10-01), pages 928 - 938, XP002407535 *

Also Published As

Publication number Publication date
WO2006127590A2 (en) 2006-11-30

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