MXPA01010905A - Oocitos extirpados de telofase para transferencia nuclear. - Google Patents
Oocitos extirpados de telofase para transferencia nuclear.Info
- Publication number
- MXPA01010905A MXPA01010905A MXPA01010905A MXPA01010905A MXPA01010905A MX PA01010905 A MXPA01010905 A MX PA01010905A MX PA01010905 A MXPA01010905 A MX PA01010905A MX PA01010905 A MXPA01010905 A MX PA01010905A MX PA01010905 A MXPA01010905 A MX PA01010905A
- Authority
- MX
- Mexico
- Prior art keywords
- oocyte
- cell
- polar
- activated
- reconstructed
- Prior art date
Links
- 210000000287 oocyte Anatomy 0.000 title claims abstract description 158
- 238000012546 transfer Methods 0.000 title claims description 38
- 230000016853 telophase Effects 0.000 title description 12
- 238000000034 method Methods 0.000 claims abstract description 68
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 25
- 210000001082 somatic cell Anatomy 0.000 claims abstract description 18
- 238000010367 cloning Methods 0.000 claims abstract description 7
- 210000004027 cell Anatomy 0.000 claims description 85
- 210000004940 nucleus Anatomy 0.000 claims description 35
- 238000011161 development Methods 0.000 claims description 19
- 210000000805 cytoplasm Anatomy 0.000 claims description 15
- 238000000338 in vitro Methods 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 230000004913 activation Effects 0.000 claims description 14
- 210000000349 chromosome Anatomy 0.000 claims description 12
- 210000003754 fetus Anatomy 0.000 claims description 12
- 230000009261 transgenic effect Effects 0.000 claims description 12
- 210000004602 germ cell Anatomy 0.000 claims description 11
- 230000003213 activating effect Effects 0.000 claims description 10
- 210000004994 reproductive system Anatomy 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 210000000801 secondary oocyte Anatomy 0.000 claims description 7
- 238000001125 extrusion Methods 0.000 claims description 6
- 230000003436 cytoskeletal effect Effects 0.000 claims description 5
- 239000003112 inhibitor Substances 0.000 claims description 5
- PGHMRUGBZOYCAA-ADZNBVRBSA-N ionomycin Chemical compound O1[C@H](C[C@H](O)[C@H](C)[C@H](O)[C@H](C)/C=C/C[C@@H](C)C[C@@H](C)C(/O)=C/C(=O)[C@@H](C)C[C@@H](C)C[C@@H](CCC(O)=O)C)CC[C@@]1(C)[C@@H]1O[C@](C)([C@@H](C)O)CC1 PGHMRUGBZOYCAA-ADZNBVRBSA-N 0.000 claims description 5
- PGHMRUGBZOYCAA-UHFFFAOYSA-N ionomycin Natural products O1C(CC(O)C(C)C(O)C(C)C=CCC(C)CC(C)C(O)=CC(=O)C(C)CC(C)CC(CCC(O)=O)C)CCC1(C)C1OC(C)(C(C)O)CC1 PGHMRUGBZOYCAA-UHFFFAOYSA-N 0.000 claims description 5
- 238000005516 engineering process Methods 0.000 claims description 4
- 230000007910 cell fusion Effects 0.000 claims description 3
- 238000000520 microinjection Methods 0.000 claims description 3
- 230000000392 somatic effect Effects 0.000 claims 1
- 241000124008 Mammalia Species 0.000 abstract description 8
- 241000283690 Bos taurus Species 0.000 description 19
- 230000004927 fusion Effects 0.000 description 17
- 230000018109 developmental process Effects 0.000 description 13
- 210000002257 embryonic structure Anatomy 0.000 description 11
- 210000001109 blastomere Anatomy 0.000 description 10
- 210000002950 fibroblast Anatomy 0.000 description 10
- 230000031864 metaphase Effects 0.000 description 10
- 208000015380 nutritional deficiency disease Diseases 0.000 description 7
- 210000002459 blastocyst Anatomy 0.000 description 6
- 239000012528 membrane Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 210000002308 embryonic cell Anatomy 0.000 description 4
- 238000002406 microsurgery Methods 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical compound C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 108010077544 Chromatin Proteins 0.000 description 3
- 210000003855 cell nucleus Anatomy 0.000 description 3
- 210000003483 chromatin Anatomy 0.000 description 3
- 230000001086 cytosolic effect Effects 0.000 description 3
- 239000012894 fetal calf serum Substances 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 230000035800 maturation Effects 0.000 description 3
- 210000000472 morula Anatomy 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 230000008672 reprogramming Effects 0.000 description 2
- 238000012800 visualization Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 101150066002 GFP gene Proteins 0.000 description 1
- 238000003309 Hoesch reaction Methods 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical class C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 230000018199 S phase Effects 0.000 description 1
- 241000242583 Scyphozoa Species 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- GBOGMAARMMDZGR-UHFFFAOYSA-N UNPD149280 Natural products N1C(=O)C23OC(=O)C=CC(O)CCCC(C)CC=CC3C(O)C(=C)C(C)C2C1CC1=CC=CC=C1 GBOGMAARMMDZGR-UHFFFAOYSA-N 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000029803 blastocyst development Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 208000031513 cyst Diseases 0.000 description 1
- GBOGMAARMMDZGR-JREHFAHYSA-N cytochalasin B Natural products C[C@H]1CCC[C@@H](O)C=CC(=O)O[C@@]23[C@H](C=CC1)[C@H](O)C(=C)[C@@H](C)[C@@H]2[C@H](Cc4ccccc4)NC3=O GBOGMAARMMDZGR-JREHFAHYSA-N 0.000 description 1
- GBOGMAARMMDZGR-TYHYBEHESA-N cytochalasin B Chemical compound C([C@H]1[C@@H]2[C@@H](C([C@@H](O)[C@@H]3/C=C/C[C@H](C)CCC[C@@H](O)/C=C/C(=O)O[C@@]23C(=O)N1)=C)C)C1=CC=CC=C1 GBOGMAARMMDZGR-TYHYBEHESA-N 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000008029 eradication Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000008175 fetal development Effects 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 108091006047 fluorescent proteins Proteins 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13146999P | 1999-04-28 | 1999-04-28 | |
| PCT/CA2000/000483 WO2000065035A1 (en) | 1999-04-28 | 2000-04-27 | Telophase enucleated oocytes for nuclear transfer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MXPA01010905A true MXPA01010905A (es) | 2002-11-07 |
Family
ID=22449596
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MXPA01010905A MXPA01010905A (es) | 1999-04-28 | 2000-04-27 | Oocitos extirpados de telofase para transferencia nuclear. |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1179053A1 (ja) |
| JP (1) | JP2002542779A (ja) |
| KR (1) | KR20020008398A (ja) |
| AU (1) | AU777799B2 (ja) |
| BR (1) | BR0010588A (ja) |
| CA (1) | CA2370385A1 (ja) |
| MX (1) | MXPA01010905A (ja) |
| NZ (1) | NZ515159A (ja) |
| WO (1) | WO2000065035A1 (ja) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379977A (zh) * | 2001-04-10 | 2002-11-20 | 陆长富 | 一种构建哺乳动物克隆胚的方法及人类克隆胚的构建 |
| JP2005508185A (ja) * | 2001-11-06 | 2005-03-31 | 上海第二医科大学 | ウサギの卵母細胞を用いる異種間体細胞核移植 |
| CN107828718B (zh) * | 2017-11-28 | 2021-08-13 | 陈子江 | 一种制备单倍体核细胞的方法 |
| CN108823067B (zh) * | 2018-07-23 | 2023-08-11 | 中国农业科学院北京畜牧兽医研究所 | 一种细胞去核显微操作针及一种细胞去核的方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9517780D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
-
2000
- 2000-04-27 CA CA002370385A patent/CA2370385A1/en not_active Abandoned
- 2000-04-27 WO PCT/CA2000/000483 patent/WO2000065035A1/en active Search and Examination
- 2000-04-27 AU AU42815/00A patent/AU777799B2/en not_active Ceased
- 2000-04-27 NZ NZ515159A patent/NZ515159A/xx unknown
- 2000-04-27 BR BR0010588-0A patent/BR0010588A/pt not_active IP Right Cessation
- 2000-04-27 EP EP00922386A patent/EP1179053A1/en not_active Withdrawn
- 2000-04-27 MX MXPA01010905A patent/MXPA01010905A/es unknown
- 2000-04-27 JP JP2000614372A patent/JP2002542779A/ja active Pending
- 2000-04-27 KR KR1020017013848A patent/KR20020008398A/ko not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| NZ515159A (en) | 2003-03-28 |
| CA2370385A1 (en) | 2000-11-02 |
| KR20020008398A (ko) | 2002-01-30 |
| JP2002542779A (ja) | 2002-12-17 |
| EP1179053A1 (en) | 2002-02-13 |
| BR0010588A (pt) | 2003-07-22 |
| WO2000065035A1 (en) | 2000-11-02 |
| AU777799B2 (en) | 2004-10-28 |
| AU4281500A (en) | 2000-11-10 |
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