MX9804418A - Nuevos almidones por via de la modificacion de la expresion de genes de la enzima biosintetica del almidon. - Google Patents

Nuevos almidones por via de la modificacion de la expresion de genes de la enzima biosintetica del almidon.

Info

Publication number
MX9804418A
MX9804418A MX9804418A MX9804418A MX9804418A MX 9804418 A MX9804418 A MX 9804418A MX 9804418 A MX9804418 A MX 9804418A MX 9804418 A MX9804418 A MX 9804418A MX 9804418 A MX9804418 A MX 9804418A
Authority
MX
Mexico
Prior art keywords
corn
starch
expression
chimeric gene
nucleic acid
Prior art date
Application number
MX9804418A
Other languages
English (en)
Other versions
MXPA98004418A (es
Inventor
Natalie Louise Hubbard
Theodore Mitchell Klein
Karen E Broglie
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of MX9804418A publication Critical patent/MX9804418A/es
Publication of MXPA98004418A publication Critical patent/MXPA98004418A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/212—Starch; Modified starch; Starch derivatives, e.g. esters or ethers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Abstract

La presente invencion expone la utilizacion de un clon de cADN para construir genes para inhibicion de la actividad enzimática de la enzima ramificante de almidon en maíz. Más específicamente, esta invencion se refiere a un método de controlar la estructura fina de almidon del almidon derivado del grano de maíz que comprende: (1) preparar un gen quimérico que contiene un fragmento de ácido nucleico que codifica un gen estructural de la enzima ramificante de almidon o un fragmento del mismo, unido operablemente en orientacion sentido o antisentido en el lado corriente arriba para un fragmento de ácido nucleico que codifica un promotor que dirige la expresion del gen en tejido de endosperma de maíz, y unido operablemente del lado corriente abajo para un fragmento deácido nucleico que codifica una secuencia reguladora adecuada para la terminacion transcripcional, y (2) transformacion de maíz con dicho gen quimérico, en donde la expresion de dicho gen quimérico resulta en la alteracion de la estructura fina de almidon derivado del grano de dicho maíz transformado comparado a la estructura fina del almidon derivado de maíz que no posee dicho gen quimérico.
MXPA/A/1998/004418A 1995-12-20 1998-06-03 Nuevos almidones por via de la modificacion de laexpresion de genes de la enzima biosintetica delalmidon MXPA98004418A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US911395P 1995-12-20 1995-12-20
US009113 1995-12-20

Publications (2)

Publication Number Publication Date
MX9804418A true MX9804418A (es) 1998-09-30
MXPA98004418A MXPA98004418A (es) 1998-11-16

Family

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Also Published As

Publication number Publication date
RO118718B1 (ro) 2003-09-30
US6376749B1 (en) 2002-04-23
EP0868520B1 (en) 2009-01-14
HUP9902112A2 (hu) 1999-10-28
BR9612086A (pt) 1999-02-17
WO1997022703A3 (en) 1997-07-24
AU1684697A (en) 1997-07-14
AR005157A1 (es) 1999-04-14
CA2239979C (en) 2007-09-25
CN1219199A (zh) 1999-06-09
CA2239979A1 (en) 1997-06-26
WO1997022703A2 (en) 1997-06-26
ZA9610590B (en) 1998-06-17
DE69637816D1 (de) 2009-03-05
ES2321347T3 (es) 2009-06-04
HUP9902112A3 (en) 2001-11-28
EP0868520A2 (en) 1998-10-07

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