MX9800500A - Inhibicion de la expresion de genes. - Google Patents

Inhibicion de la expresion de genes.

Info

Publication number
MX9800500A
MX9800500A MX9800500A MX9800500A MX9800500A MX 9800500 A MX9800500 A MX 9800500A MX 9800500 A MX9800500 A MX 9800500A MX 9800500 A MX9800500 A MX 9800500A MX 9800500 A MX9800500 A MX 9800500A
Authority
MX
Mexico
Prior art keywords
gene expression
nucleotide sequence
inhibition
sequence
intron
Prior art date
Application number
MX9800500A
Other languages
English (en)
Other versions
MXPA98000500A (es
Inventor
Peter Poulsen
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco filed Critical Danisco
Publication of MXPA98000500A publication Critical patent/MXPA98000500A/es
Publication of MX9800500A publication Critical patent/MX9800500A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01018—1,4-Alpha-glucan branching enzyme (2.4.1.18), i.e. glucan branching enzyme

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se describe un método para inhibir la expresion de genes; el método, que afecta la actividad enzimática en una planta, comprende expresar en una planta (una célula, un tejido o un organo de la misma) una secuencia de nucleotidos, caracterizado además porque la secuencia de nucleotidos se codifica, parcial o completamente, para un intron en una orientacion de antisentido; y porque la secuencia de nucleotidos no contiene una secuencia que esté en antisentido a una secuencia de exones asociada normalmente con el intron.
MX9800500A 1995-07-14 1996-07-12 Inhibicion de la expresion de genes. MX9800500A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9514435.8 1995-07-14
GBGB9514435.8A GB9514435D0 (en) 1995-07-14 1995-07-14 Inhibition of gene expression
PCT/EP1996/003052 WO1997004112A2 (en) 1995-07-14 1996-07-12 Inhibition of gene expression

Publications (2)

Publication Number Publication Date
MXPA98000500A MXPA98000500A (es) 1998-04-01
MX9800500A true MX9800500A (es) 1998-04-30

Family

ID=10777672

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9800500A MX9800500A (es) 1995-07-14 1996-07-12 Inhibicion de la expresion de genes.

Country Status (11)

Country Link
US (1) US6147279A (es)
EP (1) EP0839202A2 (es)
JP (1) JPH11509103A (es)
CN (1) CN1196093A (es)
AU (1) AU709798B2 (es)
BR (1) BR9609726A (es)
CA (1) CA2226832A1 (es)
GB (1) GB9514435D0 (es)
MX (1) MX9800500A (es)
NZ (1) NZ313626A (es)
WO (1) WO1997004112A2 (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001512972A (ja) * 1997-02-21 2001-08-28 ダニスコ エイ／エス デンプン分枝酵素発現のセンスイントロン阻害
AU738272B2 (en) * 1997-02-21 2001-09-13 Dupont Nutrition Biosciences Aps Antisense intron inhibition of starch branching enzyme expression
US6303848B1 (en) 1998-01-16 2001-10-16 Large Scale Biology Corporation Method for conferring herbicide, pest, or disease resistance in plant hosts
US6426185B1 (en) 1998-01-16 2002-07-30 Large Scale Biology Corporation Method of compiling a functional gene profile in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in an anti-sense orientation
WO2002079391A2 (en) * 2001-04-02 2002-10-10 Cytoprint, Inc. Methods and apparatus for discovering, identifying and comparing biological activity mechanisms
EP1925672A1 (en) 2001-06-22 2008-05-28 Syngeta Participations AG Abiotic stress responsive polynucleotides and polypeptides
EP2216405A1 (en) 2002-05-03 2010-08-11 Monsanto Technology LLC Speed specific USP promoters for expressing genes in plants
US7078234B2 (en) 2002-12-18 2006-07-18 Monsanto Technology Llc Maize embryo-specific promoter compositions and methods for use thereof
CN1726282B (zh) 2002-12-19 2012-09-26 拜尔作物科学股份公司 合成终粘度增加的淀粉的植物细胞及植物
BRPI0408896A (pt) 2003-03-28 2006-04-25 Monsanto Technology Llc promotores de planta para uso no desenvolvimento precoce de sementes
RU2377303C2 (ru) 2003-06-30 2009-12-27 Коммонвелт Сайентифик Энд Индастриал Рисерч Организейшн Пшеница с модифицированной активностью ветвящего фермента, а также полученные из нее крахмал и крахмалсодержащие продукты
WO2006089950A2 (en) 2005-02-26 2006-08-31 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
EP1707632A1 (de) 2005-04-01 2006-10-04 Bayer CropScience GmbH Phosphorylierte waxy-Kartoffelstärke
EP1874938B1 (en) * 2005-04-19 2012-04-04 BASF Plant Science GmbH Starchy-endosperm and/or germinating embryo-specific expression in mono-cotyledonous plants
CA2605393A1 (en) 2005-04-20 2006-10-26 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
US7790873B2 (en) 2005-05-10 2010-09-07 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
WO2008099013A1 (en) 2007-02-16 2008-08-21 Basf Plant Science Gmbh Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants
MX2011010763A (es) 2009-04-17 2011-12-16 Basf Plant Science Co Gmbh Promotor de planta operable en endosperma y usos del mismo.
CA2766858A1 (en) 2009-07-10 2011-01-13 Basf Plant Science Company Gmbh Expression cassettes for endosperm-specific expression in plants
EP2507375A4 (en) 2009-12-03 2013-04-24 Basf Plant Science Co Gmbh EXPRESSION CASSETTES FOR EMBRYOSPECIFIC EXPRESSION IN PLANTS
EP2766486A1 (en) 2011-10-12 2014-08-20 Bayer CropScience AG Plants with decreased activity of a starch dephosphorylating enzyme
WO2013053729A1 (en) 2011-10-12 2013-04-18 Bayer Cropscience Ag Plants with decreased activity of a starch dephosphorylating enzyme
KR102538812B1 (ko) 2016-05-26 2023-06-01 넌헴스 비.브이. 씨없는 과실 생산 식물
EP3806619A1 (en) 2018-06-15 2021-04-21 Nunhems B.V. Seedless watermelon plants comprising modifications in an abc transporter gene

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740463A (en) * 1984-04-13 1988-04-26 Massachusetts Institute Of Technology Methods and artificial genes for antagonizing the function of an oncogene
NZ219472A (en) * 1986-03-28 1990-08-28 Calgene Inc Regulation of phenotype in plant cells using dsdna constructs
WO1990008828A2 (en) * 1989-02-02 1990-08-09 Paladin Hybrids Inc. Molecular methods of hybrid seed production
SE9004095L (sv) * 1990-12-21 1992-06-01 Amylogene Hb Genetisk foeraendring av potatis foer bildning av staerkelse av amylostyp
DE4104782B4 (de) * 1991-02-13 2006-05-11 Bayer Cropscience Gmbh Neue Plasmide, enthaltend DNA-Sequenzen, die Veränderungen der Karbohydratkonzentration und Karbohydratzusammensetzung in Pflanzen hervorrufen, sowie Pflanzen und Pflanzenzellen enthaltend dieses Plasmide
CA2108144A1 (en) * 1991-03-06 1992-09-07 Jack A. Roth Methods and compositions for the selective inhibition of gene expression
JPH0698656A (ja) * 1992-03-30 1994-04-12 Mitsui Giyousai Shokubutsu Bio Kenkyusho:Kk 遺伝子処理したイネ
WO1994009144A1 (en) * 1992-10-14 1994-04-28 Zeneca Limited Novel plants and processes for obtaining them
GB9223454D0 (en) * 1992-11-09 1992-12-23 Ici Plc Novel plants and processes for obtaining them
NZ266386A (en) * 1993-05-11 1997-11-24 Univ North Carolina Use of antisense rna oligonucleotides in regulating gene expression
US6103893A (en) * 1994-03-25 2000-08-15 National Starch And Chemical Investment Holding Corporation High amylose starch from transgenic potato plants

Also Published As

Publication number Publication date
AU6614596A (en) 1997-02-18
GB9514435D0 (en) 1995-09-13
WO1997004112A3 (en) 1997-05-15
CA2226832A1 (en) 1997-02-06
NZ313626A (en) 1999-06-29
WO1997004112A2 (en) 1997-02-06
JPH11509103A (ja) 1999-08-17
AU709798B2 (en) 1999-09-09
BR9609726A (pt) 1999-03-16
EP0839202A2 (en) 1998-05-06
CN1196093A (zh) 1998-10-14
US6147279A (en) 2000-11-14

Similar Documents

Publication Publication Date Title
MX9800499A (es) Inhibicion de la expresion de genes.
MX9800500A (es) Inhibicion de la expresion de genes.
BR9808647A (pt) Inibição de ìntron anti-sentido de enzima de expressão de ramificação de amido
EP0832203A4 (en) USE OF NEURONAL FETAL CELL LINES FOR TRANSPLANTATION THERAPY
MX9606422A (es) Modificacion del contenido de almidon en plantas.
AU7572096A (en) Chimeric oligomers having an rna-cleavage activity
MX9702881A (es) Promotor aislado de la papa.
GB8916213D0 (en) Dna constructs,cells and plants derived therefrom
GR3015085T3 (en) Anti-sense regulation of gene expression in plant cells.
IL157644A0 (en) Method for the purification of a protein preparation with erythropoietin activity
IL72643A (en) Gene encoding an analog of human interleukin-ii which substantially retains the biological activity of natural human interleukin-ii,microorganism expression vector containing the gene,microorganisms transformed by the vector and the human interleukin-ii analog produced by the microorganisms
PT1997899T (pt) Método e meios para modular a morte celular programada em células eucarióticas
AU1002497A (en) Method of inhibiting transformation, growth and metastasis of cells in which purine metabolic enzyme activity is elevated
ES2182791T3 (es) Procedimiento y medicamento para la inhibicion de la expresion de un gen predeterminado.
GB9712415D0 (en) Genetic control of flowering
AU2273795A (en) Reduction of endogenous seed protein levels in plants
AU4940596A (en) Method to obtain male-sterile plants
WO1995001439A3 (en) Plants having modified response to ethylene
MX9801264A (es) Control de la expresion del gen de cd44 para uso terapeutico.
NZ311180A (en) Nucleotide sequence encoding a peptide having beta-xylosidase activity and use thereof
TR199802638T2 (xx) Kanser tedavisinde naaladase inhibit�rleri kullan�m�.
EP1672061A3 (en) Nucleotide sequences encoding ACC synthase enzymes
GB9519299D0 (en) Genetic strategy
BR9811014A (pt) "região concentrada reguladora de gene especìfica para semente precoce"
WO1994025593A3 (en) Method for obtaining male-sterile plants