MX9606138A - Una mezcla purificada de colagenasas y otras dos proteasas obtenidas de clostridium histolyticum. - Google Patents

Una mezcla purificada de colagenasas y otras dos proteasas obtenidas de clostridium histolyticum.

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Publication number
MX9606138A
MX9606138A MX9606138A MX9606138A MX9606138A MX 9606138 A MX9606138 A MX 9606138A MX 9606138 A MX9606138 A MX 9606138A MX 9606138 A MX9606138 A MX 9606138A MX 9606138 A MX9606138 A MX 9606138A
Authority
MX
Mexico
Prior art keywords
mixture
cells
tissue
purified
collagenases
Prior art date
Application number
MX9606138A
Other languages
English (en)
Other versions
MXPA96006138A (es
Inventor
Francis E Dwulet
Bernice B Ellis
John F Gill
Linda B Jacobsen
Marilyn E Smith
David G Waters
Original Assignee
Boehringer Mannheim Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Corp filed Critical Boehringer Mannheim Corp
Priority claimed from PCT/US1995/008858 external-priority patent/WO1996000283A1/en
Publication of MXPA96006138A publication Critical patent/MXPA96006138A/es
Publication of MX9606138A publication Critical patent/MX9606138A/es

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se describe una mezcla enzimática purificada util para aislar células o agregados celulares de tejidos. La mezcla incluye al menos dos enzimas colagenasas, al menos otras dos proteasas, y componentes adicionales diferentes a las proteasas. La mezcla se purifica removiendo al menos algunos de los componentes diferentes a las proteasas. La mezcla purificada puede usarse entonces para aislar células o agregados celulares de tejidos. También se describen los componentes esenciales de la mezcla enzimática purificada así como los intervalos y relaciones preferidas de esos componentes esenciales para aislar células agregados celulares de tejidos. Finalmente, se describe un sistema de disociacion que puede ser usado con la mezcla enzimática purificada para disociar tejidos y recuperar células o agregados celulares.
MX9606138A 1995-06-23 1995-06-23 Una mezcla purificada de colagenasas y otras dos proteasas obtenidas de clostridium histolyticum. MX9606138A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US265,292 1994-06-24
US265292 1994-06-24
PCT/US1995/008858 WO1996000283A1 (en) 1994-06-24 1995-06-23 A purified mixture of collagenases and two other proteases obtained from clostridium histolyticum

Publications (2)

Publication Number Publication Date
MXPA96006138A MXPA96006138A (es) 1998-01-01
MX9606138A true MX9606138A (es) 1998-01-31

Family

ID=39165236

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9606138A MX9606138A (es) 1995-06-23 1995-06-23 Una mezcla purificada de colagenasas y otras dos proteasas obtenidas de clostridium histolyticum.

Country Status (1)

Country Link
MX (1) MX9606138A (es)

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