MX380693B - Produccion de manool. - Google Patents
Produccion de manool.Info
- Publication number
- MX380693B MX380693B MX2017015827A MX2017015827A MX380693B MX 380693 B MX380693 B MX 380693B MX 2017015827 A MX2017015827 A MX 2017015827A MX 2017015827 A MX2017015827 A MX 2017015827A MX 380693 B MX380693 B MX 380693B
- Authority
- MX
- Mexico
- Prior art keywords
- manool
- cpp
- synthase
- seq
- contacting
- Prior art date
Links
- CECREIRZLPLYDM-QGZVKYPTSA-N manool Chemical compound CC1(C)CCC[C@]2(C)[C@@H](CC[C@](O)(C)C=C)C(=C)CC[C@H]21 CECREIRZLPLYDM-QGZVKYPTSA-N 0.000 title abstract 3
- CECREIRZLPLYDM-UHFFFAOYSA-N ent-epimanool Natural products CC1(C)CCCC2(C)C(CCC(O)(C)C=C)C(=C)CCC21 CECREIRZLPLYDM-UHFFFAOYSA-N 0.000 title 1
- JKMAMXHNJFUAFT-UHFFFAOYSA-N manool Natural products CC1(C)CCCC2(C)C(CCC(O)C=C)C(=C)CCC12 JKMAMXHNJFUAFT-UHFFFAOYSA-N 0.000 title 1
- JCAIWDXKLCEQEO-ATPOGHATSA-N 5alpha,9alpha,10beta-labda-8(20),13-dien-15-yl diphosphate Chemical compound CC1(C)CCC[C@]2(C)[C@@H](CCC(/C)=C/COP(O)(=O)OP(O)(O)=O)C(=C)CC[C@H]21 JCAIWDXKLCEQEO-ATPOGHATSA-N 0.000 abstract 3
- JCAIWDXKLCEQEO-LXOWHHAPSA-N Copalyl diphosphate Natural products [P@@](=O)(OP(=O)(O)O)(OC/C=C(\CC[C@H]1C(=C)CC[C@H]2C(C)(C)CCC[C@@]12C)/C)O JCAIWDXKLCEQEO-LXOWHHAPSA-N 0.000 abstract 3
- OINNEUNVOZHBOX-XBQSVVNOSA-N Geranylgeranyl diphosphate Natural products [P@](=O)(OP(=O)(O)O)(OC/C=C(\CC/C=C(\CC/C=C(\CC/C=C(\C)/C)/C)/C)/C)O OINNEUNVOZHBOX-XBQSVVNOSA-N 0.000 abstract 1
- 108030004291 Sclareol synthases Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 108010064741 ent-kaurene synthetase A Proteins 0.000 abstract 1
- OINNEUNVOZHBOX-KGODAQDXSA-N geranylgeranyl diphosphate Chemical compound CC(C)=CCC\C(C)=C/CC\C(C)=C\CC\C(C)=C\CO[P@@](O)(=O)OP(O)(O)=O OINNEUNVOZHBOX-KGODAQDXSA-N 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y505/00—Intramolecular lyases (5.5)
- C12Y505/01—Intramolecular lyases (5.5.1)
- C12Y505/01012—Copalyl diphosphate synthase (5.5.1.12)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y505/00—Intramolecular lyases (5.5)
- C12Y505/01—Intramolecular lyases (5.5.1)
- C12Y505/01014—Syn-copalyl-diphosphate synthase (5.5.1.14)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
La presente invención se refiere a métodos para producir (+)-manool que comprenden: poner en contacto el difosfato de geranilgeranilo con una difosfato de copalilo (CPP) sintasa para formar un difosfato de (9S,10S)-copalilo en donde la CPP sintasa comprende una secuencia de aminoácidos que tiene al menos 90 %, 95 %, 98 %, 99 y 100 % de identidad de la secuencia con un polipéptido seleccionado del grupo que consiste de la SEC ID NO:1 y la SEC ID NO:2; y poner en contacto el CPP con una enzima de esclareol sintasa para formar el (+)-manool.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562187236P | 2015-06-30 | 2015-06-30 | |
| GBGB1601249.4A GB201601249D0 (en) | 2016-01-22 | 2016-01-22 | Production of manool |
| PCT/EP2016/065448 WO2017001641A1 (en) | 2015-06-30 | 2016-06-30 | Production of manool |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2017015827A MX2017015827A (es) | 2018-04-10 |
| MX380693B true MX380693B (es) | 2025-03-12 |
Family
ID=55534814
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2017015827A MX380693B (es) | 2015-06-30 | 2016-06-30 | Produccion de manool. |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10385361B2 (es) |
| EP (1) | EP3317418B1 (es) |
| JP (1) | JP6856553B2 (es) |
| CN (1) | CN107889506B (es) |
| CA (1) | CA2987487A1 (es) |
| ES (1) | ES2933968T3 (es) |
| GB (1) | GB201601249D0 (es) |
| MX (1) | MX380693B (es) |
| WO (1) | WO2017001641A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11214775B2 (en) * | 2018-07-10 | 2022-01-04 | Firmenich Sa | Method for biocatalytic production of terpene compounds |
| US12486499B2 (en) | 2019-07-10 | 2025-12-02 | Firmenich Sa | Biocatalytic method for the controlled degradation of terpene compounds |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3681853D1 (de) | 1985-08-12 | 1991-11-14 | Firmenich & Cie | Verfahren zur herstellung von sauerstoff enthaltenden decalinderivaten. |
| CN101319220B (zh) * | 2007-06-05 | 2010-08-25 | 中国中医科学院中药研究所 | 一种丹参二萜合酶基因及其编码产物与应用 |
| CN102016051B (zh) * | 2008-01-29 | 2014-05-28 | 弗门尼舍有限公司 | 生产香紫苏醇的方法 |
| EP2366782A1 (en) * | 2010-03-09 | 2011-09-21 | Sandoz AG | Polypeptide having diterpene synthase activity |
| PL2773751T3 (pl) | 2011-11-01 | 2017-08-31 | Firmenich Sa | Cytochrom 450 i jego zastosowanie w enzymatycznym utlenianiu terpenów |
| EP3929289A1 (en) * | 2012-07-30 | 2021-12-29 | Evolva, Inc. | Sclareol and labdenediol diphosphate synthase polypeptides, encoding nucleic acid molecules and uses thereof |
-
2016
- 2016-01-22 GB GBGB1601249.4A patent/GB201601249D0/en not_active Ceased
- 2016-06-30 MX MX2017015827A patent/MX380693B/es unknown
- 2016-06-30 CA CA2987487A patent/CA2987487A1/en not_active Abandoned
- 2016-06-30 EP EP16736039.5A patent/EP3317418B1/en active Active
- 2016-06-30 ES ES16736039T patent/ES2933968T3/es active Active
- 2016-06-30 WO PCT/EP2016/065448 patent/WO2017001641A1/en not_active Ceased
- 2016-06-30 JP JP2017568149A patent/JP6856553B2/ja active Active
- 2016-06-30 CN CN201680036387.3A patent/CN107889506B/zh active Active
- 2016-06-30 US US15/737,809 patent/US10385361B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US10385361B2 (en) | 2019-08-20 |
| JP2018527892A (ja) | 2018-09-27 |
| ES2933968T3 (es) | 2023-02-15 |
| BR112017028183A2 (pt) | 2018-08-28 |
| CA2987487A1 (en) | 2017-01-05 |
| MX2017015827A (es) | 2018-04-10 |
| EP3317418A1 (en) | 2018-05-09 |
| US20190002925A1 (en) | 2019-01-03 |
| EP3317418B1 (en) | 2022-11-30 |
| WO2017001641A1 (en) | 2017-01-05 |
| GB201601249D0 (en) | 2016-03-09 |
| CN107889506A (zh) | 2018-04-06 |
| JP6856553B2 (ja) | 2021-04-07 |
| CN107889506B (zh) | 2021-09-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112016020517A2 (pt) | método de produção de lipídeo através da utilização de ss-cetoacil-acp sintase | |
| MX2017003847A (es) | Proteinas biespecificas activables por proteasas. | |
| ES2721309T3 (es) | Composición para preparar tagatosa y método para preparar tagatosa a partir de fructosa | |
| MX391311B (es) | Acidos nucleicos modificados dirigidos a acidos nucleicos. | |
| PH12017502240A1 (en) | Recombinant microorganism for improved production of alanine | |
| EP3760713A3 (en) | Lipase variants and polynucleotides encoding same | |
| MY169049A (en) | Microorganisms for producing putrescine and process for producing putrescine using them | |
| NZ783737A (en) | Novel b-galactosidase | |
| MY181068A (en) | Recombinant microorganism for improved production of fine chemicals | |
| PH12017502215A1 (en) | Recombinant microorganism for improve production of fine chemicals | |
| PH12018500150A1 (en) | Microorganisms for producing putrescine or ornithine and process for producing putrescine or ornithine using them | |
| MX2016011465A (es) | Drimenol-sintasas y metodos para producir drimenol. | |
| MX2017000014A (es) | Metodo para producir acido graso de cadena media con el uso de beta-cetoacil-proteina portadora de acilo (acp) sintasa. | |
| IN2015DN03206A (es) | ||
| BR112017008702A2 (pt) | ?mutante de endoxilanase, dna, vetor, célula, método de produção de mutantes de endoxilanase, composição enzimática e método de produção de solução de açúcar? | |
| MY197890A (en) | Mutant strains of trichoderma reesei | |
| MY182519A (en) | Method for producing lipid using acyl-acp thioesterase | |
| MY182443A (en) | Method of producing lipid | |
| MX2021011770A (es) | Produccion de manool. | |
| PH12017502323A1 (en) | Novel xylanase | |
| MX380693B (es) | Produccion de manool. | |
| IN2013MU02316A (es) | ||
| MY174840A (en) | Microorganism producing o-acetyl-homoserine, and method for producing o-acetyl-homoserine by using same | |
| MX2016013955A (es) | Drimenol-sintasas y metodo de produccion de drimenol. | |
| SG11201902998SA (en) | PROMOTER OF Hspa5 GENE |