MX2023002924A - Metodos para la identificacion de sitios de union mediante el uso de espectrometria de masas de intercambio de hidrogeno. - Google Patents

Metodos para la identificacion de sitios de union mediante el uso de espectrometria de masas de intercambio de hidrogeno.

Info

Publication number
MX2023002924A
MX2023002924A MX2023002924A MX2023002924A MX2023002924A MX 2023002924 A MX2023002924 A MX 2023002924A MX 2023002924 A MX2023002924 A MX 2023002924A MX 2023002924 A MX2023002924 A MX 2023002924A MX 2023002924 A MX2023002924 A MX 2023002924A
Authority
MX
Mexico
Prior art keywords
binding site
methods
mass spectrometry
hydrogen exchange
exchange mass
Prior art date
Application number
MX2023002924A
Other languages
English (en)
Inventor
Hui Xiao
Sisi Zhang
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2023002924A publication Critical patent/MX2023002924A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Hydrogen, Water And Hydrids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proporcionan métodos para identificar un sitio de unión entre un producto farmacéutico de proteínas y una proteína de célula hospedadora (HCP) mediante el uso de espectrometría de masas de intercambio de hidrógeno. La presente solicitud también proporciona métodos para modificar productos farmacéuticos de proteínas para eliminar la escisión o modificación mediante HCP. Además, la presente solicitud proporciona métodos para bloquear el sitio de unión identificado en productos farmacéuticos de proteínas para eliminar la escisión o modificación mediante HCP.
MX2023002924A 2020-09-11 2021-09-11 Metodos para la identificacion de sitios de union mediante el uso de espectrometria de masas de intercambio de hidrogeno. MX2023002924A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063077220P 2020-09-11 2020-09-11
PCT/US2021/049975 WO2022056336A1 (en) 2020-09-11 2021-09-11 Methods for binding site identification using hydrogen exchange mass spectrometry

Publications (1)

Publication Number Publication Date
MX2023002924A true MX2023002924A (es) 2023-04-10

Family

ID=78333247

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023002924A MX2023002924A (es) 2020-09-11 2021-09-11 Metodos para la identificacion de sitios de union mediante el uso de espectrometria de masas de intercambio de hidrogeno.

Country Status (11)

Country Link
US (1) US20220082571A1 (es)
EP (1) EP4211255A1 (es)
JP (1) JP2023542858A (es)
KR (1) KR20230066025A (es)
CN (1) CN116490777A (es)
AU (1) AU2021342274A1 (es)
BR (1) BR112023004262A2 (es)
CA (1) CA3194955A1 (es)
IL (1) IL301234A (es)
MX (1) MX2023002924A (es)
WO (1) WO2022056336A1 (es)

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4604345B2 (ja) * 2000-12-21 2011-01-05 住友化学株式会社 タンパク質のｎ末端のアミノ酸配列決定方法
CA2436503A1 (en) * 2001-02-01 2002-08-08 Huw A. Davies Improved methods for protein identification, characterization and sequencing by tandem mass spectrometry
AU2003221860A1 (en) * 2002-04-10 2003-10-27 The Regents Of The University Of California Improved methods for high-resolution identification of solvent-accessible amide hydrogens in polypeptides and for characterization of polypeptide structure
US7378396B2 (en) * 2004-08-11 2008-05-27 The Cleveland Clinic Foundation Therapeutic agents and methods for cardiovascular disease
WO2009050266A2 (en) * 2007-10-19 2009-04-23 Pronota N.V. Use of n-terminal and c-terminal proteomics technology to enhance protein therapeutics and diagnostics
EP2394173B1 (en) * 2009-02-09 2015-03-25 Roche Glycart AG Immunoglobulin glycosylation pattern analysis
MX2013013054A (es) * 2011-05-12 2014-02-20 Genentech Inc Metodo de monitoreo de lc-ms/ms de reaccion multiple para detectar anticuerpos terapeuticos en muestras de animales utilizando peptidos de firma de estructura.
WO2015120036A1 (en) * 2014-02-04 2015-08-13 University Of Virginia Patent Foundation Compositions and methods for analysis of protein sequences and post-translational modifications
EP3387440B1 (en) * 2015-12-09 2021-05-12 F. Hoffmann-La Roche AG Method for determining the in vivo interaction mode
CN108369232B (zh) * 2015-12-09 2022-05-31 豪夫迈·罗氏有限公司 人IgG1结合多聚抗原的直接亲和力测量
US20190169675A1 (en) * 2016-08-12 2019-06-06 Lonza Ltd Proteomic analysis of host cell proteins

Also Published As

Publication number Publication date
WO2022056336A1 (en) 2022-03-17
AU2021342274A1 (en) 2023-04-27
CN116490777A (zh) 2023-07-25
EP4211255A1 (en) 2023-07-19
KR20230066025A (ko) 2023-05-12
JP2023542858A (ja) 2023-10-12
CA3194955A1 (en) 2022-03-17
IL301234A (en) 2023-05-01
US20220082571A1 (en) 2022-03-17
BR112023004262A2 (pt) 2023-04-04

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