MX2023000960A - Metodos y composiciones para la produccion de productos derivados del acetil-coa. - Google Patents

Metodos y composiciones para la produccion de productos derivados del acetil-coa.

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Publication number
MX2023000960A
MX2023000960A MX2023000960A MX2023000960A MX2023000960A MX 2023000960 A MX2023000960 A MX 2023000960A MX 2023000960 A MX2023000960 A MX 2023000960A MX 2023000960 A MX2023000960 A MX 2023000960A MX 2023000960 A MX2023000960 A MX 2023000960A
Authority
MX
Mexico
Prior art keywords
production
acetyl
compositions
methods
derived products
Prior art date
Application number
MX2023000960A
Other languages
English (en)
Inventor
Michael David Lynch
Shaui Li
Original Assignee
Univ Duke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Duke filed Critical Univ Duke
Publication of MX2023000960A publication Critical patent/MX2023000960A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0067—Oxidoreductases (1.) acting on hydrogen as donor (1.12)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
    • C—CHEMISTRY; METALLURGY
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    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04001—Pyruvate dehydrogenase (acetyl-transferring) (1.2.4.1)
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    • C12Y112/00—Oxidoreductases acting on hydrogen as donor (1.12)
    • C12Y112/07—Oxidoreductases acting on hydrogen as donor (1.12) with an iron-sulfur protein as acceptor (1.12.7)
    • C12Y112/07002—Ferredoxin hydrogenase (1.12.7.2)
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    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03001—Citrate (Si)-synthase (2.3.3.1)
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2800/101—Plasmid DNA for bacteria
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Cepas microbianas modificadas genéticamente y bioprocesos relacionados para la producción de productos a partir de acetil-CoA. Específicamente, el uso de válvulas anabólicas controladas dinámicamente para reducir la actividad de ciertas enzimas conduce a una mayor producción de productos en un proceso de dos etapas.
MX2023000960A 2020-07-24 2021-07-23 Metodos y composiciones para la produccion de productos derivados del acetil-coa. MX2023000960A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063056031P 2020-07-24 2020-07-24
PCT/US2021/043023 WO2022020748A1 (en) 2020-07-24 2021-07-23 Methods and compositions for the production of acetyl-coa derived products

Publications (1)

Publication Number Publication Date
MX2023000960A true MX2023000960A (es) 2023-04-26

Family

ID=79728388

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023000960A MX2023000960A (es) 2020-07-24 2021-07-23 Metodos y composiciones para la produccion de productos derivados del acetil-coa.

Country Status (14)

Country Link
US (1) US20230227864A1 (es)
EP (1) EP4176044A4 (es)
JP (1) JP2023538225A (es)
KR (1) KR20230041779A (es)
CN (1) CN116134127A (es)
AU (1) AU2021312935A1 (es)
BR (1) BR112023001032A2 (es)
CA (1) CA3189923A1 (es)
CR (1) CR20230098A (es)
DO (1) DOP2023000013A (es)
IL (1) IL300042A (es)
MX (1) MX2023000960A (es)
WO (1) WO2022020748A1 (es)
ZA (1) ZA202302090B (es)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012031639A2 (pt) * 2010-06-11 2015-11-24 Regentes Of The University Of California vias sintéticas para a síntese de biocombustíveis
SI2855687T1 (sl) * 2012-06-04 2020-09-30 Genomatica, Inc. Mikroorganizmi in metode za proizvodnjo 4-hidroksibutirat, 1,4-butandiola in sorodne spojine
WO2014057008A1 (en) * 2012-10-09 2014-04-17 Chalmers Intellectual Property Rights Ab Engineering of acetyl-coenzyme a metabolism in yeast
ES2937642T3 (es) * 2014-06-11 2023-03-30 Univ Duke Composiciones y métodos para el control de flujo rápido y dinámico usando válvulas metabólicas sintéticas
US10731185B2 (en) * 2016-03-22 2020-08-04 University Of Georgia Research Foundation, Inc. Genetically engineered microbes and methods for producing citramalate

Also Published As

Publication number Publication date
CA3189923A1 (en) 2022-01-27
ZA202302090B (en) 2023-06-28
BR112023001032A2 (pt) 2023-03-28
US20230227864A1 (en) 2023-07-20
KR20230041779A (ko) 2023-03-24
AU2021312935A1 (en) 2023-02-23
WO2022020748A1 (en) 2022-01-27
EP4176044A1 (en) 2023-05-10
JP2023538225A (ja) 2023-09-07
IL300042A (en) 2023-03-01
DOP2023000013A (es) 2023-04-30
EP4176044A4 (en) 2024-02-21
CN116134127A (zh) 2023-05-16
CR20230098A (es) 2023-08-07

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