MX2023000136A - Métodos para preparar muestras para análisis proteómico. - Google Patents
Métodos para preparar muestras para análisis proteómico.Info
- Publication number
- MX2023000136A MX2023000136A MX2023000136A MX2023000136A MX2023000136A MX 2023000136 A MX2023000136 A MX 2023000136A MX 2023000136 A MX2023000136 A MX 2023000136A MX 2023000136 A MX2023000136 A MX 2023000136A MX 2023000136 A MX2023000136 A MX 2023000136A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- proteins
- protein sample
- sample
- proteomic analysis
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000000575 proteomic method Methods 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 7
- 108090000623 proteins and genes Proteins 0.000 abstract 7
- 239000008280 blood Substances 0.000 abstract 3
- 210000004369 blood Anatomy 0.000 abstract 3
- 239000003146 anticoagulant agent Substances 0.000 abstract 2
- 229940127219 anticoagulant drug Drugs 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 239000003223 protective agent Substances 0.000 abstract 2
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000002532 enzyme inhibitor Substances 0.000 abstract 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención se refiere a métodos para preparar una muestra de proteína para análisis proteómico. En modalidades ejemplares, el método comprende (a) poner en contacto una muestra de sangre que comprende proteínas con un agente protector que comprende un anticoagulante (AC) y un liberador de aldehído (AR), para obtener una mezcla, opcionalmente, en la que la muestra de sangre se agrega a una muestra de sangre, tubo de recolección (BCT) que comprende el agente protector, y (b) aislar una fracción que comprende proteínas o una fuente de proteínas de la mezcla para producir una muestra de proteína o una fuente de una muestra de proteína, donde las etapas del método se llevan a cabo en el ausencia de inhibidores de enzimas proteolíticas exógenas, donde la muestra de proteína es adecuada para análisis proteómico y peptidomédico.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202063047143P | 2020-07-01 | 2020-07-01 | |
PCT/US2021/039870 WO2022006260A1 (en) | 2020-07-01 | 2021-06-30 | Methods of preparing samples for proteomic analysis |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2023000136A true MX2023000136A (es) | 2023-05-19 |
Family
ID=77398630
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2023000136A MX2023000136A (es) | 2020-07-01 | 2021-06-30 | Métodos para preparar muestras para análisis proteómico. |
Country Status (6)
Country | Link |
---|---|
US (1) | US20230314442A1 (es) |
EP (1) | EP4176268A1 (es) |
CN (1) | CN116157681A (es) |
CA (1) | CA3184070A1 (es) |
MX (1) | MX2023000136A (es) |
WO (1) | WO2022006260A1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3903106A1 (en) * | 2018-12-27 | 2021-11-03 | Streck, Inc. | Methods of preparing samples for proteomic analysis |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2540649B2 (ja) | 1990-04-27 | 1996-10-09 | テルモ株式会社 | 採血管 |
US5257633A (en) | 1992-06-23 | 1993-11-02 | Becton, Dickinson And Company | Surface modified blood collection tubes |
US6077235A (en) | 1999-02-23 | 2000-06-20 | Becton, Dickinson And Company | Blood collection assembly and method therefor |
US6551267B1 (en) | 2000-10-18 | 2003-04-22 | Becton, Dickinson And Company | Medical article having blood-contacting surface |
NO2398912T3 (es) | 2009-02-18 | 2018-02-10 | ||
EP3468361A4 (en) * | 2016-06-08 | 2020-02-19 | CF Genome, LLC | CHEMICAL COMPOSITION FOR STABILIZING EXTRACELLULAR VESICLES IN A BLOOD SAMPLE, AND METHOD OF USING THE SAME |
EP3576625A4 (en) | 2017-02-03 | 2020-12-16 | Streck, Inc. | SAMPLE COLLECTION TUBE WITH PRESERVATIVE |
CN111372451B (zh) * | 2017-10-19 | 2022-08-12 | 斯特雷克股份有限公司 | 用于胞外囊泡的溶血和凝血调节以及稳定化的组合物 |
EP3903106A1 (en) * | 2018-12-27 | 2021-11-03 | Streck, Inc. | Methods of preparing samples for proteomic analysis |
-
2021
- 2021-06-30 CN CN202180052919.3A patent/CN116157681A/zh active Pending
- 2021-06-30 US US18/012,054 patent/US20230314442A1/en active Pending
- 2021-06-30 MX MX2023000136A patent/MX2023000136A/es unknown
- 2021-06-30 CA CA3184070A patent/CA3184070A1/en active Pending
- 2021-06-30 WO PCT/US2021/039870 patent/WO2022006260A1/en unknown
- 2021-06-30 EP EP21756090.3A patent/EP4176268A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CN116157681A (zh) | 2023-05-23 |
WO2022006260A1 (en) | 2022-01-06 |
US20230314442A1 (en) | 2023-10-05 |
EP4176268A1 (en) | 2023-05-10 |
CA3184070A1 (en) | 2022-01-06 |
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