MX2022013704A - Procedimientos para mejorar la salud de especies porcinas mediante la inactivacion dirigida de cd163. - Google Patents
Procedimientos para mejorar la salud de especies porcinas mediante la inactivacion dirigida de cd163.Info
- Publication number
- MX2022013704A MX2022013704A MX2022013704A MX2022013704A MX2022013704A MX 2022013704 A MX2022013704 A MX 2022013704A MX 2022013704 A MX2022013704 A MX 2022013704A MX 2022013704 A MX2022013704 A MX 2022013704A MX 2022013704 A MX2022013704 A MX 2022013704A
- Authority
- MX
- Mexico
- Prior art keywords
- gene
- methods
- health
- improving
- prrsv
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000002779 inactivation Effects 0.000 title 1
- 101150087379 CD163 gene Proteins 0.000 abstract 3
- 108700028369 Alleles Proteins 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 2
- 241001135989 Porcine reproductive and respiratory syndrome virus Species 0.000 abstract 2
- 241000282887 Suidae Species 0.000 abstract 2
- 241000282898 Sus scrofa Species 0.000 abstract 1
- 238000010362 genome editing Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8778—Swine embryos
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0656—Adult fibroblasts
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
- A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/108—Swine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2320/00—Applications; Uses
- C12N2320/30—Special therapeutic applications
- C12N2320/34—Allele or polymorphism specific uses
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- C12N2510/00—Genetically modified cells
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/91—Cell lines ; Processes using cell lines
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Animal Husbandry (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Rheumatology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063020128P | 2020-05-05 | 2020-05-05 | |
| US202063021370P | 2020-05-07 | 2020-05-07 | |
| PCT/GB2021/051060 WO2021224599A1 (en) | 2020-05-05 | 2021-05-04 | Methods for improving the health of porcine species by targeted inactivation of cd163 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2022013704A true MX2022013704A (es) | 2022-12-07 |
Family
ID=75954141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2022013704A MX2022013704A (es) | 2020-05-05 | 2021-05-04 | Procedimientos para mejorar la salud de especies porcinas mediante la inactivacion dirigida de cd163. |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US11208659B2 (https=) |
| EP (1) | EP4145991A1 (https=) |
| JP (1) | JP2023525263A (https=) |
| KR (1) | KR20230006827A (https=) |
| CN (5) | CN118773199A (https=) |
| AU (1) | AU2021268263A1 (https=) |
| BR (1) | BR112022020072A2 (https=) |
| CA (1) | CA3179710A1 (https=) |
| MX (1) | MX2022013704A (https=) |
| WO (1) | WO2021224599A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3179710A1 (en) * | 2020-05-05 | 2021-11-11 | Andrew Mark CIGAN | Methods for improving the health of porcine species by targeted inactivation of cd163 |
| US20250064032A1 (en) | 2021-12-10 | 2025-02-27 | Pig Improvement Company Uk Limited | Editing tmprss2/4 for disease resistance in livestock |
| WO2024097603A2 (en) * | 2022-11-02 | 2024-05-10 | Abs Global, Inc. | Method of creating gene edited animals comprising a wild type allele |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4873191A (en) | 1981-06-12 | 1989-10-10 | Ohio University | Genetic transformation of zygotes |
| PT2709445T (pt) | 2011-05-16 | 2018-11-27 | Univ Missouri | Animais resistentes ao vírus da síntese reprodutiva e respiratória porcino |
| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| US20140068727A1 (en) | 2012-09-05 | 2014-03-06 | Apple Inc. | Wi-fi credential sharing using images |
| GB201313235D0 (en) * | 2013-07-24 | 2013-09-04 | Univ Edinburgh | Antiviral Compositions Methods and Animals |
| CN104593422A (zh) * | 2015-01-08 | 2015-05-06 | 中国农业大学 | 一种抗蓝耳病克隆猪的制备方法 |
| CA3255239A1 (en) | 2015-08-06 | 2026-03-02 | The Curators Of The University Of Missouri | Pathogen-resistant animals having modified cd163 genes |
| RS65691B1 (sr) * | 2015-08-06 | 2024-07-31 | Univ Missouri | Svinja i ćelije rezistentne na virus reproduktivnog i respiratornog sindroma svinja (prrsv) koje imaju modifikovane cd163 gene |
| GB201617559D0 (en) * | 2016-10-17 | 2016-11-30 | University Court Of The University Of Edinburgh The | Swine comprising modified cd163 and associated methods |
| CN107177595A (zh) * | 2017-06-07 | 2017-09-19 | 浙江大学 | 用于猪CD163基因编辑的靶向sgRNA、修饰载体及其制备方法和应用 |
| CN107354170A (zh) * | 2017-08-24 | 2017-11-17 | 中国农业科学院北京畜牧兽医研究所 | 一种基因敲除载体以及制备cd163基因敲除猪成纤维细胞的方法 |
| CN108103099B (zh) * | 2017-12-18 | 2022-03-04 | 中山大学 | 一种抗蓝耳病Marc-145细胞系及其制备方法和应用 |
| KR102680060B1 (ko) | 2018-04-17 | 2024-07-02 | 더 큐레이터스 오브 더 유니버시티 오브 미주리 | 바이러스에 의한 감염으로부터 돼지 태아를 보호하는 방법 |
| CN108823248A (zh) * | 2018-04-20 | 2018-11-16 | 中山大学 | 一种利用CRISPR/Cas9编辑陆川猪CD163基因的方法 |
| CN108753832A (zh) * | 2018-04-20 | 2018-11-06 | 中山大学 | 一种利用CRISPR/Cas9编辑大白猪CD163基因的方法 |
| CN109055385B (zh) * | 2018-08-21 | 2021-08-27 | 吉林大学 | 一种有效抑制ⅱ型prrsv感染的基因序列及其应用 |
| CN109666646A (zh) * | 2018-11-15 | 2019-04-23 | 广东省农业科学院农业生物基因研究中心 | 一种cd163基因编辑的猪胚胎成纤维细胞系的制备及应用 |
| CN110951745A (zh) * | 2019-10-30 | 2020-04-03 | 内蒙古大学 | Cd163突变基因及其在抑制或阻断猪产生抗体的方法和应用 |
| CA3179710A1 (en) * | 2020-05-05 | 2021-11-11 | Andrew Mark CIGAN | Methods for improving the health of porcine species by targeted inactivation of cd163 |
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2021
- 2021-05-04 CA CA3179710A patent/CA3179710A1/en active Pending
- 2021-05-04 WO PCT/GB2021/051060 patent/WO2021224599A1/en not_active Ceased
- 2021-05-04 MX MX2022013704A patent/MX2022013704A/es unknown
- 2021-05-04 BR BR112022020072A patent/BR112022020072A2/pt unknown
- 2021-05-04 US US17/307,369 patent/US11208659B2/en active Active
- 2021-05-04 KR KR1020227038847A patent/KR20230006827A/ko active Pending
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| US20210348165A1 (en) | 2021-11-11 |
| CN118773199A (zh) | 2024-10-15 |
| US20220042017A1 (en) | 2022-02-10 |
| US20230272397A1 (en) | 2023-08-31 |
| US11535850B2 (en) | 2022-12-27 |
| CN117487855B (zh) | 2024-07-26 |
| US12371692B2 (en) | 2025-07-29 |
| BR112022020072A2 (pt) | 2022-11-29 |
| KR20230006827A (ko) | 2023-01-11 |
| US20260092277A1 (en) | 2026-04-02 |
| EP4145991A1 (en) | 2023-03-15 |
| JP2023525263A (ja) | 2023-06-15 |
| US20220135976A1 (en) | 2022-05-05 |
| AU2021268263A1 (en) | 2022-10-27 |
| US12416005B2 (en) | 2025-09-16 |
| US11208659B2 (en) | 2021-12-28 |
| CN113151291B (zh) | 2024-09-03 |
| CN113151291A (zh) | 2021-07-23 |
| CN118879712A (zh) | 2024-11-01 |
| CN117487855A (zh) | 2024-02-02 |
| WO2021224599A1 (en) | 2021-11-11 |
| CN118620905A (zh) | 2024-09-10 |
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