MX2022005413A - Aparatos y métodos para la recuperación de células únicas basados en láser a partir de arreglos microcapilares. - Google Patents

Aparatos y métodos para la recuperación de células únicas basados en láser a partir de arreglos microcapilares.

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Publication number
MX2022005413A
MX2022005413A MX2022005413A MX2022005413A MX2022005413A MX 2022005413 A MX2022005413 A MX 2022005413A MX 2022005413 A MX2022005413 A MX 2022005413A MX 2022005413 A MX2022005413 A MX 2022005413A MX 2022005413 A MX2022005413 A MX 2022005413A
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Mexico
Prior art keywords
microcapillary
laser
methods
single cell
based single
Prior art date
Application number
MX2022005413A
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English (en)
Inventor
Bob Chen
Ryan Lewis Kelly
Austin Blanco
Frances Liu
Derek Croote
Samuel Timothy Hatch
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Xcella Biosciences Inc
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Publication date
Application filed by Xcella Biosciences Inc filed Critical Xcella Biosciences Inc
Publication of MX2022005413A publication Critical patent/MX2022005413A/es

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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50857—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using arrays or bundles of open capillaries for holding samples
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    • G01N1/44—Sample treatment involving radiation, e.g. heat
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    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
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    • G01N33/5047—Cells of the immune system
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Abstract

Se proporcionan sistemas y métodos para recuperar el contenido de una muestra de un arreglo microcapilar. El arreglo microcapilar incluye una pluralidad de pozos microcapilares. Se coloca un primer láser apuntando hacia un primer pozo microcapilar en la pluralidad de pozos microcapilares. El láser pulsa al menos una vez en el primer pozo microcapilar. Se extrae el contenido del primer pozo microcapilar, recuperando el contenido del primer pozo microcapilar.
MX2022005413A 2019-11-08 2020-11-06 Aparatos y métodos para la recuperación de células únicas basados en láser a partir de arreglos microcapilares. MX2022005413A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962932989P 2019-11-08 2019-11-08
PCT/US2020/059485 WO2021092442A1 (en) 2019-11-08 2020-11-06 Apparatus and methods for laser-based single cell recovery from microcapillary arrays

Publications (1)

Publication Number Publication Date
MX2022005413A true MX2022005413A (es) 2022-06-17

Family

ID=75848692

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022005413A MX2022005413A (es) 2019-11-08 2020-11-06 Aparatos y métodos para la recuperación de células únicas basados en láser a partir de arreglos microcapilares.

Country Status (10)

Country Link
US (1) US20220373440A1 (es)
EP (1) EP4055359A4 (es)
JP (1) JP2022554355A (es)
KR (1) KR20220102626A (es)
CN (1) CN114945812A (es)
AU (1) AU2020378091A1 (es)
CA (1) CA3156776A1 (es)
IL (1) IL292781A (es)
MX (1) MX2022005413A (es)
WO (1) WO2021092442A1 (es)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000011024A2 (en) * 1998-08-21 2000-03-02 Surromed, Inc. Novel optical architectures for microvolume laser-scanning cytometers
US9657290B2 (en) * 2012-07-03 2017-05-23 The Board Of Trustees Of The Leland Stanford Junior University Scalable bio-element analysis
WO2016134370A1 (en) * 2015-02-22 2016-08-25 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening apparatus, process, and products
WO2017078484A1 (ko) * 2015-11-04 2017-05-11 주식회사 셀레믹스 분자클론 추출 및 확인 방법
GB201603088D0 (en) * 2016-02-23 2016-04-06 Isis Innovation Cell sorting
JP2018017657A (ja) * 2016-07-29 2018-02-01 国立研究開発法人理化学研究所 細胞分泌物の分析方法、マルチウェルプレート
WO2018053485A1 (en) * 2016-09-19 2018-03-22 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening and sorting apparatus, process, and products
WO2018119043A1 (en) * 2016-12-20 2018-06-28 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening apparatus, process, and products
CN107589105B (zh) * 2017-07-21 2021-08-24 中国科学院青岛生物能源与过程研究所 一种用于单细胞快速拉曼测量和激光弹射分选的一体化装置

Also Published As

Publication number Publication date
EP4055359A1 (en) 2022-09-14
US20220373440A1 (en) 2022-11-24
WO2021092442A1 (en) 2021-05-14
JP2022554355A (ja) 2022-12-28
KR20220102626A (ko) 2022-07-20
EP4055359A4 (en) 2023-04-05
CN114945812A (zh) 2022-08-26
AU2020378091A1 (en) 2022-06-02
IL292781A (en) 2022-07-01
CA3156776A1 (en) 2021-05-14

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