MX2022002521A - Metodos y composiciones para la modificacion y administracion de linfocitos. - Google Patents

Metodos y composiciones para la modificacion y administracion de linfocitos.

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Publication number
MX2022002521A
MX2022002521A MX2022002521A MX2022002521A MX2022002521A MX 2022002521 A MX2022002521 A MX 2022002521A MX 2022002521 A MX2022002521 A MX 2022002521A MX 2022002521 A MX2022002521 A MX 2022002521A MX 2022002521 A MX2022002521 A MX 2022002521A
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MX
Mexico
Prior art keywords
cells
lymphocytes
compositions
methods
polynucleotides
Prior art date
Application number
MX2022002521A
Other languages
English (en)
Inventor
James Joseph Onuffer
Gregory Ian Frost
Farzad Haerizadeh
Frederic Vigant
Anirban Kundu
Original Assignee
Exuma Biotech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2019/049259 external-priority patent/WO2020047527A2/en
Application filed by Exuma Biotech Corp filed Critical Exuma Biotech Corp
Publication of MX2022002521A publication Critical patent/MX2022002521A/es

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
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    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
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    • A61K39/463—Cellular immunotherapy characterised by recombinant expression
    • A61K39/4631—Chimeric Antigen Receptors [CAR]
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    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464402—Receptors, cell surface antigens or cell surface determinants
    • A61K39/464411—Immunoglobulin superfamily
    • A61K39/464412—CD19 or B4
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    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464402—Receptors, cell surface antigens or cell surface determinants
    • A61K39/464411—Immunoglobulin superfamily
    • A61K39/464413—CD22, BL-CAM, siglec-2 or sialic acid binding Ig-related lectin 2
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    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464402—Receptors, cell surface antigens or cell surface determinants
    • A61K39/464424—CD20
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K2039/80—Vaccine for a specifically defined cancer
    • A61K2039/804—Blood cells [leukemia, lymphoma]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K39/46
    • A61K2239/31—Indexing codes associated with cellular immunotherapy of group A61K39/46 characterized by the route of administration
    • A—HUMAN NECESSITIES
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    • C—CHEMISTRY; METALLURGY
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    • C07K2319/00—Fusion polypeptide
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    • C12N2740/10011—Retroviridae
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    • C12Y302/01035—Hyaluronoglucosaminidase (3.2.1.35), i.e. hyaluronidase

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  • Developmental Biology & Embryology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente divulgación proporciona métodos y composiciones para modificar genéticamente linfocitos, por ejemplo, linfocitos T y/o células NK. En algunas formas de realización, los métodos incluyen 5 mezclas de reacción y formulaciones celulares resultantes, que se crean usando sangre entera o un componente de esta que no sea una PBMC, y comprenden además linfocitos T y particulares retrovirales recombinantes que tienen polinucleótidos que codifican un CAR. En algunas formas de realización, los linfocitos modificados se reintroducen en un sujeto por vía subcutánea. En algunas formas de realización, se proporcionan polinucleótidos que proporcionan a los linfocitos T la capacidad de regular la 10 supervivencia y proliferación celulares en respuesta a la fijación a un CAR.
MX2022002521A 2019-09-01 2020-08-31 Metodos y composiciones para la modificacion y administracion de linfocitos. MX2022002521A (es)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201962894852P 2019-09-01 2019-09-01
US201962894849P 2019-09-01 2019-09-01
US201962894853P 2019-09-01 2019-09-01
US201962894926P 2019-09-02 2019-09-02
PCT/US2019/049259 WO2020047527A2 (en) 2018-09-02 2019-09-02 Methods and compositions for genetically modifying lymphocytes in blood or in enriched pbmcs
US201962943207P 2019-12-03 2019-12-03
US202062985741P 2020-03-05 2020-03-05
PCT/US2020/048843 WO2021042072A1 (en) 2019-09-01 2020-08-31 Methods and compositions for the modification and delivery of lymphocytes

Publications (1)

Publication Number Publication Date
MX2022002521A true MX2022002521A (es) 2022-06-14

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US (1) US20220340927A1 (es)
EP (1) EP4022035A4 (es)
JP (1) JP2022546101A (es)
KR (1) KR20220070449A (es)
CN (1) CN116057181A (es)
AU (1) AU2020336230A1 (es)
BR (1) BR112022003617A2 (es)
CA (1) CA3152780A1 (es)
MX (1) MX2022002521A (es)
WO (1) WO2021042072A1 (es)

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US11325948B2 (en) 2016-03-19 2022-05-10 Exuma Biotech Corp. Methods and compositions for genetically modifying lymphocytes to express polypeptides comprising the intracellular domain of MPL
WO2023015310A2 (en) * 2021-08-06 2023-02-09 Seattle Children's Hospital D/B/A Seattle Children's Research Institute T-cell manufacturing methods
WO2023133595A2 (en) 2022-01-10 2023-07-13 Sana Biotechnology, Inc. Methods of ex vivo dosing and administration of lipid particles or viral vectors and related systems and uses
WO2023168305A1 (en) * 2022-03-01 2023-09-07 Exuma Biotech Corp. Viral particles with membrane-bound hyaluronidase
WO2024026377A1 (en) 2022-07-27 2024-02-01 Sana Biotechnology, Inc. Methods of transduction using a viral vector and inhibitors of antiviral restriction factors
WO2024081167A1 (en) * 2022-10-10 2024-04-18 Kite Pharma, Inc. New large scale car-t immune cell manufacturing method utilizing lentiviral vector transfection

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CA2972806A1 (en) * 2014-12-31 2016-07-07 Anthrogenesis Corporation Methods of treating hematological disorders, solid tumors, or infectious diseases using natural killer cells
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US10596274B2 (en) * 2016-03-19 2020-03-24 Exuma Biotech Corp. Methods and compositions for transducing lymphocytes and regulated expansion thereof
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CA3033788A1 (en) * 2016-08-17 2018-02-22 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
EP3872180A1 (en) * 2016-10-20 2021-09-01 Alpine Immune Sciences, Inc. Secretable variant immunomodulatory proteins and engineered cell therapy
CN111479921A (zh) * 2017-09-18 2020-07-31 埃克苏马生物技术公司 用于以基因方式修饰且扩增淋巴细胞以及调节其活性的方法及组合物
CN117004573A (zh) * 2017-12-13 2023-11-07 苏州康聚生物科技有限公司 一种包含肿瘤抗原识别受体的免疫细胞及其应用

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BR112022003617A2 (pt) 2022-09-13
EP4022035A4 (en) 2024-03-27
AU2020336230A1 (en) 2022-03-31
JP2022546101A (ja) 2022-11-02
WO2021042072A1 (en) 2021-03-04
EP4022035A1 (en) 2022-07-06
US20220340927A1 (en) 2022-10-27
CN116057181A (zh) 2023-05-02
CA3152780A1 (en) 2021-03-04
KR20220070449A (ko) 2022-05-31

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