MX2022000113A - Proceso para la desproteccion de oligonucleotidos. - Google Patents

Proceso para la desproteccion de oligonucleotidos.

Info

Publication number
MX2022000113A
MX2022000113A MX2022000113A MX2022000113A MX2022000113A MX 2022000113 A MX2022000113 A MX 2022000113A MX 2022000113 A MX2022000113 A MX 2022000113A MX 2022000113 A MX2022000113 A MX 2022000113A MX 2022000113 A MX2022000113 A MX 2022000113A
Authority
MX
Mexico
Prior art keywords
oligonucleotides
deprotection
oligonucleotide
acid
purification
Prior art date
Application number
MX2022000113A
Other languages
English (en)
Inventor
Sung Won Kim
Kyeong Eun Jung
Yong Rag Choi
Younggoo Kang
Pascal Schmidt
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2022000113A publication Critical patent/MX2022000113A/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B01D15/203—Equilibration or regeneration
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La invención se refiere a un nuevo proceso para la purificación de oligonucleótidos, que comprende la eliminación de un grupo protector 5' hidroxi lábil al ácido en el extremo 5'-O-oligonucleótido del oligonucleótido mediante una desprotección en columna con un ácido.
MX2022000113A 2019-07-09 2020-07-06 Proceso para la desproteccion de oligonucleotidos. MX2022000113A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19185225 2019-07-09
PCT/EP2020/068922 WO2021004977A1 (en) 2019-07-09 2020-07-06 Process for the deprotection of oligonucleotides

Publications (1)

Publication Number Publication Date
MX2022000113A true MX2022000113A (es) 2022-02-10

Family

ID=67226011

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022000113A MX2022000113A (es) 2019-07-09 2020-07-06 Proceso para la desproteccion de oligonucleotidos.

Country Status (12)

Country Link
US (1) US20220410035A1 (es)
EP (1) EP3997100A1 (es)
JP (1) JP7311642B2 (es)
KR (1) KR20220009437A (es)
CN (1) CN114051499A (es)
AU (1) AU2020309307B2 (es)
BR (1) BR112021026636A2 (es)
CA (1) CA3139610A1 (es)
IL (1) IL289440A (es)
MX (1) MX2022000113A (es)
TW (1) TW202117013A (es)
WO (1) WO2021004977A1 (es)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7273933B1 (en) * 1998-02-26 2007-09-25 Isis Pharmaceuticals, Inc. Methods for synthesis of oligonucleotides
US20050136458A1 (en) * 1998-12-31 2005-06-23 Oligos, Etc, Inc. Method for nucleic acid preparation
CA2479901A1 (en) * 2002-03-21 2003-10-02 Avecia Biotechnology Inc Purification methods for oligonucleotides and their analogs
US6989442B2 (en) * 2002-07-12 2006-01-24 Sirna Therapeutics, Inc. Deprotection and purification of oligonucleotides and their derivatives
US7655790B2 (en) * 2002-07-12 2010-02-02 Sirna Therapeutics, Inc. Deprotection and purification of oligonucleotides and their derivatives
US7345163B2 (en) * 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
AU2006319847A1 (en) * 2005-12-02 2007-06-07 Nihon Medi-Physics Co., Ltd. Process for production of compound labeled with radioactive fluorine
CA2632133A1 (en) * 2005-12-06 2007-06-14 Nihon Medi-Physics Co., Ltd. Process for production of compound labeled with radioactive fluorine
EP3658155A4 (en) * 2017-07-28 2021-06-30 Nogra Pharma Limited PROCESS FOR THE PREPARATION OF OLIGONUCLEOTIDIC COMPOUNDS
SG11202110045PA (en) * 2019-03-20 2021-10-28 Wave Life Sciences Ltd Technologies useful for oligonucleotide preparation

Also Published As

Publication number Publication date
KR20220009437A (ko) 2022-01-24
CN114051499A (zh) 2022-02-15
BR112021026636A2 (pt) 2022-03-22
JP2022537182A (ja) 2022-08-24
IL289440A (en) 2022-02-01
CA3139610A1 (en) 2021-01-14
EP3997100A1 (en) 2022-05-18
AU2020309307B2 (en) 2023-03-30
TW202117013A (zh) 2021-05-01
AU2020309307A1 (en) 2021-11-04
JP7311642B2 (ja) 2023-07-19
WO2021004977A1 (en) 2021-01-14
US20220410035A1 (en) 2022-12-29

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