MX2021010137A - Plantas de cannabis resistentes al mildiu polvoriento. - Google Patents

Plantas de cannabis resistentes al mildiu polvoriento.

Info

Publication number
MX2021010137A
MX2021010137A MX2021010137A MX2021010137A MX2021010137A MX 2021010137 A MX2021010137 A MX 2021010137A MX 2021010137 A MX2021010137 A MX 2021010137A MX 2021010137 A MX2021010137 A MX 2021010137A MX 2021010137 A MX2021010137 A MX 2021010137A
Authority
MX
Mexico
Prior art keywords
powdery mildew
cannabis plant
cannabis plants
mildew resistant
genome modification
Prior art date
Application number
MX2021010137A
Other languages
English (en)
Inventor
Tal Sherman
Ido Margalit
Shira Corem
Original Assignee
Betterseeds Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Betterseeds Ltd filed Critical Betterseeds Ltd
Publication of MX2021010137A publication Critical patent/MX2021010137A/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/02Flowers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A01H1/1245Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
    • A01H1/1255Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance for fungal resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/28Cannabaceae, e.g. cannabis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physiology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La presente invención describe una planta de cannabis modificada que presenta una resistencia mejorada al mildiú polvoriento (MP). La planta de cannabis modificada antes mencionada comprende una modificación genómica direccionada que confiere una expresión reducida de al menos un alelo MLO de cannabis (CsMLO) en comparación con una planta de cannabis que carece de dicha modificación genómica direccionada. La presente invención describe además métodos para la producción de la planta de cannabis modificada usando la modificación genómica.
MX2021010137A 2019-02-23 2020-02-20 Plantas de cannabis resistentes al mildiu polvoriento. MX2021010137A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962809584P 2019-02-23 2019-02-23
PCT/IL2020/050189 WO2020170251A1 (en) 2019-02-23 2020-02-20 Powdery mildew resistant cannabis plants

Publications (1)

Publication Number Publication Date
MX2021010137A true MX2021010137A (es) 2021-09-23

Family

ID=72143539

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021010137A MX2021010137A (es) 2019-02-23 2020-02-20 Plantas de cannabis resistentes al mildiu polvoriento.

Country Status (10)

Country Link
US (1) US20220090118A1 (es)
EP (1) EP3927145A4 (es)
CN (1) CN115942867A (es)
AU (1) AU2020225594A1 (es)
CA (1) CA3131231A1 (es)
CO (1) CO2021011318A2 (es)
IL (1) IL285707B2 (es)
MX (1) MX2021010137A (es)
WO (1) WO2020170251A1 (es)
ZA (1) ZA202106234B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021046154A1 (en) * 2019-09-06 2021-03-11 James Berman Genetic modification of plants
US20230125534A1 (en) * 2019-11-04 2023-04-27 Agriculture Victoria Services Pty Ltd A Method to Produce Targeted Gene Editing Constructs
CN112746073A (zh) * 2020-12-09 2021-05-04 华中农业大学 一种通过多基因编辑获得高抗白粉病黄瓜种质材料的方法
US20240117450A1 (en) * 2021-02-09 2024-04-11 Phylos Bioscience, Inc. Powdery mildew markers for cannabis
CA3212318A1 (en) * 2021-05-07 2022-10-11 Amit DEOKAR Cannabis plants exhibiting powdery mildew resistance and methods for obtaining same
GB202211773D0 (en) * 2022-08-11 2022-09-28 Puregene Ag Quantitative trait locus associated with a pathogen resistance trait in cannabis

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA200000952A1 (ru) * 1998-03-17 2001-06-25 Новартис Аг Гены, кодирующие протеины mlo и обусловливающие устойчивость растений к грибам
US6211433B1 (en) * 1998-07-07 2001-04-03 Pioneer Hi-Bred International, Inc. Manipulation of Mlo genes to enhance disease resistance in plants
AU5815500A (en) * 1999-06-18 2001-01-09 Novartis Ag Genes controlling diseases
CN102719447A (zh) * 2012-03-31 2012-10-10 常熟市支塘镇新盛技术咨询服务有限公司 亚麻mlo型抗白粉病基因快速鉴定
CA2992799A1 (en) * 2015-07-17 2017-01-26 Institute Of Genetics And Developmental Biology Chinese Academy Of Sciences Modified plants
US20230193305A1 (en) * 2019-02-23 2023-06-22 Betterseeds Ltd Methods for increasing powdery mildew resistance in cannabis
CA3212318A1 (en) * 2021-05-07 2022-10-11 Amit DEOKAR Cannabis plants exhibiting powdery mildew resistance and methods for obtaining same
CA3166209A1 (en) * 2021-08-19 2023-02-19 BetterSeeds Ltd. Methods for increasing powdery mildew resistance in cannabis

Also Published As

Publication number Publication date
EP3927145A1 (en) 2021-12-29
CA3131231A1 (en) 2020-08-27
WO2020170251A1 (en) 2020-08-27
IL285707B2 (en) 2024-04-01
ZA202106234B (en) 2023-05-31
IL285707B1 (en) 2023-12-01
CN115942867A (zh) 2023-04-07
US20220090118A1 (en) 2022-03-24
EP3927145A4 (en) 2022-08-10
IL285707A (en) 2021-10-31
AU2020225594A1 (en) 2021-08-26
CO2021011318A2 (es) 2021-09-09

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