CO2021011318A2 - Plantas de cannabis resistentes al mildiú polvoriento - Google Patents

Plantas de cannabis resistentes al mildiú polvoriento

Info

Publication number
CO2021011318A2
CO2021011318A2 CONC2021/0011318A CO2021011318A CO2021011318A2 CO 2021011318 A2 CO2021011318 A2 CO 2021011318A2 CO 2021011318 A CO2021011318 A CO 2021011318A CO 2021011318 A2 CO2021011318 A2 CO 2021011318A2
Authority
CO
Colombia
Prior art keywords
powdery mildew
cannabis plant
plants resistant
genomic modification
cannabis plants
Prior art date
Application number
CONC2021/0011318A
Other languages
English (en)
Inventor
Tal Sherman
Ido Margalit
Shira Corem
Original Assignee
Betterseeds Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Betterseeds Ltd filed Critical Betterseeds Ltd
Publication of CO2021011318A2 publication Critical patent/CO2021011318A2/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/02—Flowers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A01H1/1245—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
    • A01H1/1255—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance for fungal resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/28—Cannabaceae, e.g. cannabis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physiology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La presente invención describe una planta de cannabis modificada que presenta una resistencia mejorada al mildiú polvoriento (MP). La planta de cannabis modificada antes mencionada comprende una modificación genómica direccionada que confiere una expresión reducida de al menos un alelo MLO de cannabis (CsMLO) en comparación con una planta de cannabis que carece de dicha modificación genómica direccionada. La presente invención describe además métodos para la producción de la planta de cannabis modificada usando la modificación genómica.
CONC2021/0011318A 2019-02-23 2021-08-27 Plantas de cannabis resistentes al mildiú polvoriento CO2021011318A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962809584P 2019-02-23 2019-02-23
PCT/IL2020/050189 WO2020170251A1 (en) 2019-02-23 2020-02-20 Powdery mildew resistant cannabis plants

Publications (1)

Publication Number Publication Date
CO2021011318A2 true CO2021011318A2 (es) 2021-09-09

Family

ID=72143539

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2021/0011318A CO2021011318A2 (es) 2019-02-23 2021-08-27 Plantas de cannabis resistentes al mildiú polvoriento

Country Status (10)

Country Link
US (1) US20220090118A1 (es)
EP (1) EP3927145A4 (es)
CN (1) CN115942867A (es)
AU (1) AU2020225594A1 (es)
CA (1) CA3131231A1 (es)
CO (1) CO2021011318A2 (es)
IL (1) IL285707B2 (es)
MX (1) MX2021010137A (es)
WO (1) WO2020170251A1 (es)
ZA (1) ZA202106234B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220356484A1 (en) * 2019-09-06 2022-11-10 Dr. James Berman Genetic modification of plants
EP4055172A1 (en) * 2019-11-04 2022-09-14 Agriculture Victoria Services Pty Ltd A method to produce targeted gene editing constructs
CN112746073A (zh) * 2020-12-09 2021-05-04 华中农业大学 一种通过多基因编辑获得高抗白粉病黄瓜种质材料的方法
US20240117450A1 (en) * 2021-02-09 2024-04-11 Phylos Bioscience, Inc. Powdery mildew markers for cannabis
CA3212318A1 (en) * 2021-05-07 2022-10-11 Amit DEOKAR Cannabis plants exhibiting powdery mildew resistance and methods for obtaining same

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TR200002537T2 (tr) * 1998-03-17 2000-11-21 Novartis Ag. Mlo proteinlerini kodlayan ve bitki üzerinde mantar direnci veren genler
US6576814B1 (en) * 1998-07-07 2003-06-10 Pioneer Hi-Bred International, Inc. Manipulation of Mlo genes to enhance disease resistance in plants
AU5815500A (en) * 1999-06-18 2001-01-09 Novartis Ag Genes controlling diseases
CN102719447A (zh) * 2012-03-31 2012-10-10 常熟市支塘镇新盛技术咨询服务有限公司 亚麻mlo型抗白粉病基因快速鉴定
CA2992799A1 (en) * 2015-07-17 2017-01-26 Institute Of Genetics And Developmental Biology Chinese Academy Of Sciences Modified plants
US20230193305A1 (en) * 2019-02-23 2023-06-22 Betterseeds Ltd Methods for increasing powdery mildew resistance in cannabis
CA3212318A1 (en) * 2021-05-07 2022-10-11 Amit DEOKAR Cannabis plants exhibiting powdery mildew resistance and methods for obtaining same
CA3166209A1 (en) * 2021-08-19 2023-02-19 BetterSeeds Ltd. Methods for increasing powdery mildew resistance in cannabis

Also Published As

Publication number Publication date
CA3131231A1 (en) 2020-08-27
AU2020225594A1 (en) 2021-08-26
CN115942867A (zh) 2023-04-07
ZA202106234B (en) 2023-05-31
IL285707B1 (en) 2023-12-01
IL285707A (en) 2021-10-31
EP3927145A4 (en) 2022-08-10
US20220090118A1 (en) 2022-03-24
EP3927145A1 (en) 2021-12-29
MX2021010137A (es) 2021-09-23
IL285707B2 (en) 2024-04-01
WO2020170251A1 (en) 2020-08-27

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