MX2021005702A - Multiplexión de variantes virales altamente evolutivas con sherlock. - Google Patents

Multiplexión de variantes virales altamente evolutivas con sherlock.

Info

Publication number
MX2021005702A
MX2021005702A MX2021005702A MX2021005702A MX2021005702A MX 2021005702 A MX2021005702 A MX 2021005702A MX 2021005702 A MX2021005702 A MX 2021005702A MX 2021005702 A MX2021005702 A MX 2021005702A MX 2021005702 A MX2021005702 A MX 2021005702A
Authority
MX
Mexico
Prior art keywords
detection method
viral variants
sherlock detection
multiplexing
evolving viral
Prior art date
Application number
MX2021005702A
Other languages
English (en)
Inventor
Cameron Myhrvold
Catherine Amanda Freije
Hayden Metsky
Pardis Sabeti
Original Assignee
Massachusetts Inst Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology
Publication of MX2021005702A publication Critical patent/MX2021005702A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

La presente invención se refiere a métodos para generar cebadores y/o sondas para uso en el análisis de una muestra que puede comprender una secuencia de objetivo de patógeno, que incluyen identificar conjuntos pan-virales de cebadores y/o sondas.
MX2021005702A 2018-11-14 2019-11-14 Multiplexión de variantes virales altamente evolutivas con sherlock. MX2021005702A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862767076P 2018-11-14 2018-11-14
PCT/US2019/061574 WO2020102608A2 (en) 2018-11-14 2019-11-14 Multiplexing highly evolving viral variants with sherlock

Publications (1)

Publication Number Publication Date
MX2021005702A true MX2021005702A (es) 2021-09-21

Family

ID=68916538

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021005702A MX2021005702A (es) 2018-11-14 2019-11-14 Multiplexión de variantes virales altamente evolutivas con sherlock.

Country Status (11)

Country Link
US (1) US20220002789A1 (es)
EP (1) EP3880844A2 (es)
JP (1) JP2022507573A (es)
KR (1) KR20210104043A (es)
CN (1) CN113302312A (es)
AU (1) AU2019380590A1 (es)
CA (1) CA3119971A1 (es)
IL (1) IL283211A (es)
MX (1) MX2021005702A (es)
SG (1) SG11202105082SA (es)
WO (1) WO2020102608A2 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112501256A (zh) * 2020-12-03 2021-03-16 台州市中心医院（台州学院附属医院） 一种CRSPR-cas13a驱动的基于双酶信号扩增策略的RNA快速检测方法
CN113680406B (zh) * 2021-08-26 2022-04-15 清华大学 一种微流控芯片多指标检测方法

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NZ520579A (en) 1997-10-24 2004-08-27 Invitrogen Corp Recombinational cloning using nucleic acids having recombination sites and methods for synthesizing double stranded nucleic acids
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
PL2611042T3 (pl) 2004-01-27 2015-06-30 Altivera L L C Pasek do testu immunologicznego mający etykiety identyfikacji radiowej (RFID)
US20100137163A1 (en) 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
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WO2008149176A1 (en) 2007-06-06 2008-12-11 Cellectis Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof
JP5546112B2 (ja) 2008-07-07 2014-07-09 キヤノン株式会社 眼科撮像装置および眼科撮像方法
WO2011139335A1 (en) 2010-04-26 2011-11-10 Sangamo Biosciences, Inc. Genome editing of a rosa locus using zinc-finger nucleases
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WO2017040316A1 (en) * 2015-08-28 2017-03-09 The Broad Institute, Inc. Sample analysis, presence determination of a target sequence
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US11618928B2 (en) * 2017-04-12 2023-04-04 The Broad Institute, Inc. CRISPR effector system based diagnostics for malaria detection

Also Published As

Publication number Publication date
CN113302312A (zh) 2021-08-24
US20220002789A1 (en) 2022-01-06
JP2022507573A (ja) 2022-01-18
IL283211A (en) 2021-06-30
AU2019380590A1 (en) 2021-07-01
EP3880844A2 (en) 2021-09-22
KR20210104043A (ko) 2021-08-24
WO2020102608A3 (en) 2020-07-30
CA3119971A1 (en) 2020-05-22
SG11202105082SA (en) 2021-06-29
AU2019380590A2 (en) 2021-07-08
WO2020102608A2 (en) 2020-05-22

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