SG11202105082SA - Multiplexing highly evolving viral variants with sherlock detection method - Google Patents

Multiplexing highly evolving viral variants with sherlock detection method

Info

Publication number
SG11202105082SA
SG11202105082SA SG11202105082SA SG11202105082SA SG11202105082SA SG 11202105082S A SG11202105082S A SG 11202105082SA SG 11202105082S A SG11202105082S A SG 11202105082SA SG 11202105082S A SG11202105082S A SG 11202105082SA SG 11202105082S A SG11202105082S A SG 11202105082SA
Authority
SG
Singapore
Prior art keywords
detection method
viral variants
sherlock detection
multiplexing
evolving viral
Prior art date
Application number
SG11202105082SA
Inventor
Catherine Amanda Freije
Hayden Metsky
Pardis Sabeti
Cameron Myhrvold
Original Assignee
Harvard College
Massachusetts Inst Technology
Broad Inst Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Massachusetts Inst Technology, Broad Inst Inc filed Critical Harvard College
Publication of SG11202105082SA publication Critical patent/SG11202105082SA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
SG11202105082SA 2018-11-14 2019-11-14 Multiplexing highly evolving viral variants with sherlock detection method SG11202105082SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862767076P 2018-11-14 2018-11-14
PCT/US2019/061574 WO2020102608A2 (en) 2018-11-14 2019-11-14 Multiplexing highly evolving viral variants with sherlock

Publications (1)

Publication Number Publication Date
SG11202105082SA true SG11202105082SA (en) 2021-06-29

Family

ID=68916538

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202105082SA SG11202105082SA (en) 2018-11-14 2019-11-14 Multiplexing highly evolving viral variants with sherlock detection method

Country Status (11)

Country Link
US (1) US20220002789A1 (en)
EP (1) EP3880844A2 (en)
JP (1) JP2022507573A (en)
KR (1) KR20210104043A (en)
CN (1) CN113302312A (en)
AU (1) AU2019380590A1 (en)
CA (1) CA3119971A1 (en)
IL (1) IL283211A (en)
MX (1) MX2021005702A (en)
SG (1) SG11202105082SA (en)
WO (1) WO2020102608A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112501256A (en) * 2020-12-03 2021-03-16 台州市中心医院(台州学院附属医院) CRSPR-cas13a driven RNA rapid detection method based on double-enzyme signal amplification strategy
CN113680406B (en) * 2021-08-26 2022-04-15 清华大学 Multi-index detection method for micro-fluidic chip

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DE69836092T2 (en) 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad RECOMBINATORY CLONING USING NUCLEAR FACILITIES WITH RECOMBINATION CENTERS
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
CN1914823A (en) 2004-01-27 2007-02-14 阿尔特维拉有限公司 Diagnostic radio frequency identification sensors and applications thereof
WO2007081386A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use
AU2007210152A1 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
EP2047910B1 (en) 2006-05-11 2012-01-11 Raindance Technologies, Inc. Microfluidic device and method
WO2008149176A1 (en) 2007-06-06 2008-12-11 Cellectis Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof
JP5546112B2 (en) 2008-07-07 2014-07-09 キヤノン株式会社 Ophthalmic imaging apparatus and ophthalmic imaging method
JP5952263B2 (en) 2010-04-26 2016-07-13 サンガモ バイオサイエンシーズ, インコーポレイテッド Genome editing of the ROSA locus using zinc finger nuclease
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
EP2898071A4 (en) 2012-09-21 2016-07-20 Broad Inst Inc Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets
WO2014047561A1 (en) 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents
SG10201912328UA (en) 2012-12-12 2020-02-27 Broad Inst Inc Delivery, Engineering and Optimization of Systems, Methods and Compositions for Sequence Manipulation and Therapeutic Applications
WO2014143158A1 (en) 2013-03-13 2014-09-18 The Broad Institute, Inc. Compositions and methods for labeling of agents
US10981167B2 (en) 2015-03-18 2021-04-20 The Broad Institute, Inc. Massively parallel on-chip coalescence of microemulsions
US11332783B2 (en) * 2015-08-28 2022-05-17 The Broad Institute, Inc. Sample analysis, presence determination of a target sequence
EP3455357A1 (en) 2016-06-17 2019-03-20 The Broad Institute Inc. Type vi crispr orthologs and systems
US20190330706A1 (en) 2016-08-26 2019-10-31 The Broad Institute, Inc. Nucleic acid amplification assays for detection of pathogens
AU2018234832A1 (en) * 2017-03-15 2019-10-10 Massachusetts Institute Of Technology CRISPR effector system based diagnostics for virus detection
US11174515B2 (en) * 2017-03-15 2021-11-16 The Broad Institute, Inc. CRISPR effector system based diagnostics
US11618928B2 (en) * 2017-04-12 2023-04-04 The Broad Institute, Inc. CRISPR effector system based diagnostics for malaria detection

Also Published As

Publication number Publication date
JP2022507573A (en) 2022-01-18
US20220002789A1 (en) 2022-01-06
WO2020102608A2 (en) 2020-05-22
AU2019380590A2 (en) 2021-07-08
KR20210104043A (en) 2021-08-24
WO2020102608A3 (en) 2020-07-30
AU2019380590A1 (en) 2021-07-01
MX2021005702A (en) 2021-09-21
CN113302312A (en) 2021-08-24
CA3119971A1 (en) 2020-05-22
IL283211A (en) 2021-06-30
EP3880844A2 (en) 2021-09-22

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