MX2021004370A - Creacion de codigos de barras de acidos nucleicos. - Google Patents

Creacion de codigos de barras de acidos nucleicos.

Info

Publication number
MX2021004370A
MX2021004370A MX2021004370A MX2021004370A MX2021004370A MX 2021004370 A MX2021004370 A MX 2021004370A MX 2021004370 A MX2021004370 A MX 2021004370A MX 2021004370 A MX2021004370 A MX 2021004370A MX 2021004370 A MX2021004370 A MX 2021004370A
Authority
MX
Mexico
Prior art keywords
nucleotide sequence
primer
nucleic acids
amplifying
nucleic acid
Prior art date
Application number
MX2021004370A
Other languages
English (en)
Inventor
Melinda Jasper
Steven Myers
Original Assignee
Perkinelmer Health Sciences Australia Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2018903923A external-priority patent/AU2018903923A0/en
Application filed by Perkinelmer Health Sciences Australia Pty Ltd filed Critical Perkinelmer Health Sciences Australia Pty Ltd
Publication of MX2021004370A publication Critical patent/MX2021004370A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/15—Modifications characterised by incorporating a consensus or conserved sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente divulgación se refiere a métodos, kits y productos para la creación de códigos de barras de ácidos nucleicos. En determinadas modalidades, la presente descripción proporciona un método para producir ácido nucleico para secuenciación utilizando amplificación clonal en un sustrato sólido, comprendiendo el método: (a) proporcionar una muestra de ácido nucleico para secuenciación; (b) amplificar la muestra de ácido nucleico usando un cebador amplificador que comprende una secuencia de nucleótidos degenerada y una secuencia de nucleótidos fija en 5', para producir ácidos nucleicos amplificados; y (c) amplificar adicionalmente los ácidos nucleicos amplificados con (i) un primer cebador que comprende la secuencia de nucleótidos fija en 5' y una primera secuencia de nucleótidos de adaptador, y (ii) un segundo cebador que comprende la secuencia de nucleótidos fija en 5' y una segunda secuencia de nucleótidos de adaptador, en donde la primera secuencia de nucleótidos de adaptador o la segunda secuencia de nucleótidos de adaptador proporciona una secuencia para el cebado posterior de la síntesis de ADN a partir de una secuencia de nucleótidos unida al sustrato sólido y la otra secuencia de nucleótidos de adaptador proporciona una secuencia para el cebado posterior de la síntesis de ADN a partir de una plantilla producida a partir del cebado posterior, y en donde uno o más del primer cebador, el segundo cebador y el cebador amplificador comprenden una secuencia de identificador específica para identificar ácidos nucleicos amplificados con el primer cebador, el segundo cebador y/o el cebador amplificador; produciendo así ácido nucleico para secuenciación utilizando amplificación clonal en el sustrato sólido.
MX2021004370A 2018-10-17 2019-10-16 Creacion de codigos de barras de acidos nucleicos. MX2021004370A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2018903923A AU2018903923A0 (en) 2018-10-17 Barcoding of nucleic acids
PCT/AU2019/051121 WO2020077400A1 (en) 2018-10-17 2019-10-16 Barcoding of nucleic acids

Publications (1)

Publication Number Publication Date
MX2021004370A true MX2021004370A (es) 2021-08-19

Family

ID=70282826

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021004370A MX2021004370A (es) 2018-10-17 2019-10-16 Creacion de codigos de barras de acidos nucleicos.

Country Status (9)

Country Link
US (1) US20210381027A1 (es)
EP (1) EP3867402A4 (es)
JP (1) JP2022508834A (es)
KR (1) KR20210079309A (es)
CN (1) CN112912514A (es)
AU (1) AU2019359771A1 (es)
IL (1) IL281941A (es)
MX (1) MX2021004370A (es)
WO (1) WO2020077400A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113166757A (zh) * 2019-02-15 2021-07-23 宝生物工程（美国)有限公司 核酸文库的制备和分析方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103060924B (zh) * 2011-10-18 2016-04-20 深圳华大基因科技有限公司 微量核酸样本的文库制备方法及其应用
WO2016010856A1 (en) * 2014-07-15 2016-01-21 Qiagen Sciences, Llc Semi-random barcodes for nucleic acid analysis
CN107699957B (zh) * 2017-05-27 2021-07-23 领星生物科技（上海）有限公司 基于dna的融合基因定量测序建库、检测方法及其应用

Also Published As

Publication number Publication date
EP3867402A4 (en) 2022-07-13
EP3867402A1 (en) 2021-08-25
US20210381027A1 (en) 2021-12-09
WO2020077400A1 (en) 2020-04-23
CN112912514A (zh) 2021-06-04
JP2022508834A (ja) 2022-01-19
AU2019359771A1 (en) 2021-05-06
IL281941A (en) 2021-05-31
KR20210079309A (ko) 2021-06-29

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