MX2021000105A - Enzimas de biosintesis de luciferina y su uso. - Google Patents

Enzimas de biosintesis de luciferina y su uso.

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Publication number
MX2021000105A
MX2021000105A MX2021000105A MX2021000105A MX2021000105A MX 2021000105 A MX2021000105 A MX 2021000105A MX 2021000105 A MX2021000105 A MX 2021000105A MX 2021000105 A MX2021000105 A MX 2021000105A MX 2021000105 A MX2021000105 A MX 2021000105A
Authority
MX
Mexico
Prior art keywords
cells
transgenic organisms
nucleic acids
fungal
luciferin
Prior art date
Application number
MX2021000105A
Other languages
English (en)
Inventor
Il''ya Viktorovich Yampol''skiy
Original Assignee
Light Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Light Bio Inc filed Critical Light Bio Inc
Publication of MX2021000105A publication Critical patent/MX2021000105A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C07K4/00—Peptides having up to 20 amino acids in an undefined or only partially defined sequence; Derivatives thereof
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    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N9/0004—Oxidoreductases (1.)
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
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    • C12N9/10—Transferases (2.)
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    • C12N9/93—Ligases (6)
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    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
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    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C12P17/167—Heterorings having sulfur atoms as ring heteroatoms, e.g. vitamin B1, thiamine nucleus and open chain analogs
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    • C12P7/00—Preparation of oxygen-containing organic compounds
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    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13008—Flavin-containing monooxygenase (1.14.13.8), i.e. dimethylaniline-monooxygenase
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    • C07K2319/00—Fusion polypeptide
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    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
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    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase

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  • Chemical & Material Sciences (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención tiene como objetivo la identificación de nuevas enzimas de biosíntesis de luciferina fúngica, ácidos nucleicos capaces de codificar estas enzimas y proteínas capaces de catalizar ciertas etapas de la biosíntesis de luciferina fúngica. La invención también proporciona la aplicación de ácidos nucleicos para producir dichas enzimas en una célula u organismo. También se proporcionan métodos para la preparación in vitro o in vivo de compuestos químicos idénticos a las luciferinas y preluciferinas fúngicas. También se proporcionan los vectores que comprenden el ácido nucleico descritos en la presente invención. Además, la presente invención proporciona casetes de expresión que comprenden el ácido nucleico de la presente invención y elementos reguladores necesarios para la expresión del ácido nucleico en una célula hospedera seleccionada. Además, también se proporcionan células, líneas celulares estables, organismos transgénicos (por ejemplo, plantas, animales, hongos o microorganismos) que incluyen ácidos nucleicos, vectores o casetes de expresión de la presente invención. La presente invención también proporciona combinaciones de ácidos nucleicos para obtener células, líneas celulares u organismos transgénicos autónomos luminosos. En modalidades preferidas, las células u organismos transgénicos son capaces de producir luciferina fúngica a partir de precursores. En algunas modalidades, las células u organismos transgénicos son capaces de producir preluciferina fúngica a partir de precursores. En algunas modalidades, las células u organismos transgénicos son capaces de bioluminiscencia en presencia de un precursor de luciferina fúngico. En algunas modalidades, las células u organismos transgénicos son capaces de bioluminiscencia autónoma. También se proporcionan combinaciones de proteínas para producir luciferina o sus precursores a partir de compuestos químicos más simples. También se proporciona un kit que contiene ácidos nucleicos, vectores o casetes de expresión de la presente invención para producir células luminosas, líneas celulares u organismos transgénicos.
MX2021000105A 2018-06-28 2019-06-27 Enzimas de biosintesis de luciferina y su uso. MX2021000105A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2018123601A RU2730038C2 (ru) 2018-06-28 2018-06-28 Ферменты биосинтеза люциферина и их применение
PCT/RU2019/050097 WO2020005120A1 (ru) 2018-06-28 2019-06-27 Ферменты биосинтеза люциферина и их применение

Publications (1)

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MX2021000105A true MX2021000105A (es) 2021-05-27

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ID=68985133

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MX2021000105A MX2021000105A (es) 2018-06-28 2019-06-27 Enzimas de biosintesis de luciferina y su uso.

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US (2) US20210115476A1 (es)
EP (1) EP3816282A4 (es)
JP (1) JP2021529536A (es)
KR (1) KR20210038888A (es)
CN (1) CN112673101A (es)
AU (1) AU2019292668A1 (es)
BR (1) BR112020026792A2 (es)
CA (1) CA3104848A1 (es)
MX (1) MX2021000105A (es)
RU (1) RU2730038C2 (es)
WO (1) WO2020005120A1 (es)

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FR3105977B1 (fr) * 2020-01-07 2022-02-11 Woodlight Méthode de fabrication de plantes bioluminescentes
US20240093206A1 (en) * 2021-03-15 2024-03-21 490 BioTech, Inc. System of stable gene expression in cell lines and methods of making and using the same
CN114480451B (zh) * 2021-12-28 2024-04-16 安徽中医药大学 一种何首乌查尔酮合酶基因FmCHS2及其编码产物和应用
WO2024034619A1 (ja) * 2022-08-10 2024-02-15 国立大学法人大阪大学 発光タンパク質

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KR20210038888A (ko) 2021-04-08
CA3104848A1 (en) 2020-01-02
BR112020026792A2 (pt) 2021-03-30
RU2018123601A3 (es) 2019-12-30
CN112673101A (zh) 2021-04-16
EP3816282A1 (en) 2021-05-05
RU2730038C2 (ru) 2020-08-14
WO2020005120A1 (ru) 2020-01-02
US20210115476A1 (en) 2021-04-22
AU2019292668A1 (en) 2021-02-18
EP3816282A4 (en) 2022-11-23
US20240117385A1 (en) 2024-04-11
JP2021529536A (ja) 2021-11-04
RU2018123601A (ru) 2019-12-30

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