MX2018013808A - Metodo analitico para cuantificacion de bacterias viables contenidas en composiciones para terapia de restauracion de microbiota (mrt). - Google Patents
Metodo analitico para cuantificacion de bacterias viables contenidas en composiciones para terapia de restauracion de microbiota (mrt).Info
- Publication number
- MX2018013808A MX2018013808A MX2018013808A MX2018013808A MX2018013808A MX 2018013808 A MX2018013808 A MX 2018013808A MX 2018013808 A MX2018013808 A MX 2018013808A MX 2018013808 A MX2018013808 A MX 2018013808A MX 2018013808 A MX2018013808 A MX 2018013808A
- Authority
- MX
- Mexico
- Prior art keywords
- viable bacteria
- quanitification
- mrt
- compositions
- analytical method
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2545/00—Reactions characterised by their quantitative nature
- C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
- C12Q2545/113—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an external standard/control, i.e. control reaction is separated from the test/target reaction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
Abstract
Cuantificación de los microorganismos bacterianos viables en un producto de fármaco para el suministro por la vía de un tubo gastro-nasal, un enema y/o una cápsula o tableta. Un procedimiento de base molecular, tal como los ensayos de PMA (monazida de propidio)-qPCR (reacción en cadena de polimerasa cuantitativa) puede ser útil para la cuantificación de bacterias viables. Al utilizar el tratamiento de PMA en combinación con qPCR, se puede determinar el número de células bacterianas viables en la muestra.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662336184P | 2016-05-13 | 2016-05-13 | |
PCT/US2017/032555 WO2017197364A1 (en) | 2016-05-13 | 2017-05-12 | Analytical method for quanitification of viable bacteria contained in microbiota restoration therapy (mrt) compositions |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2018013808A true MX2018013808A (es) | 2019-03-28 |
Family
ID=58765992
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2018013808A MX2018013808A (es) | 2016-05-13 | 2017-05-12 | Metodo analitico para cuantificacion de bacterias viables contenidas en composiciones para terapia de restauracion de microbiota (mrt). |
Country Status (12)
Country | Link |
---|---|
US (1) | US20170327862A1 (es) |
EP (1) | EP3455373A1 (es) |
JP (1) | JP2019520804A (es) |
KR (1) | KR20190003996A (es) |
CN (1) | CN109415760A (es) |
AU (1) | AU2017261820B2 (es) |
BR (1) | BR112018073330A2 (es) |
CA (1) | CA3022847A1 (es) |
IL (1) | IL262847A (es) |
MX (1) | MX2018013808A (es) |
RU (2) | RU2020106956A (es) |
WO (1) | WO2017197364A1 (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2018187272A1 (en) | 2017-04-03 | 2018-10-11 | Gusto Global, Llc | Rational design of microbial-based biotherapeutics |
WO2019213595A1 (en) | 2018-05-04 | 2019-11-07 | Ferring B.V. | Microbiota restoration therapy (mrt) compositions and methods of manufacture |
WO2019241523A1 (en) | 2018-06-14 | 2019-12-19 | Rebiotix, Inc. | Microbiota restoration therapy (mrt) compositions and methods of manufacture |
CN109811040A (zh) * | 2019-02-21 | 2019-05-28 | 北京农业智能装备技术研究中心 | 一种基于pma染色的病菌定量检测方法 |
WO2020218557A1 (ja) * | 2019-04-26 | 2020-10-29 | bitBiome株式会社 | 単一生物単位の生菌由来核酸の選択的検出、カウント、ゲノム解析 |
WO2022051610A1 (en) | 2020-09-04 | 2022-03-10 | Ferring B.V. | Microbiota restoration therapy (mrt) compositions and methods of manufacture |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6330032B2 (ja) | 2013-06-05 | 2018-05-23 | レビオティクス インコーポレイテッドRebiotix,Inc. | 微生物叢回復療法組成物を製造、処理、および梱包するための方法 |
-
2017
- 2017-05-12 MX MX2018013808A patent/MX2018013808A/es unknown
- 2017-05-12 CA CA3022847A patent/CA3022847A1/en not_active Abandoned
- 2017-05-12 EP EP17725479.4A patent/EP3455373A1/en not_active Withdrawn
- 2017-05-12 CN CN201780043039.3A patent/CN109415760A/zh active Pending
- 2017-05-12 JP JP2018559875A patent/JP2019520804A/ja active Pending
- 2017-05-12 KR KR1020187036155A patent/KR20190003996A/ko not_active Application Discontinuation
- 2017-05-12 RU RU2020106956A patent/RU2020106956A/ru unknown
- 2017-05-12 BR BR112018073330-0A patent/BR112018073330A2/pt not_active IP Right Cessation
- 2017-05-12 RU RU2018143995A patent/RU2714841C1/ru active
- 2017-05-12 WO PCT/US2017/032555 patent/WO2017197364A1/en unknown
- 2017-05-12 AU AU2017261820A patent/AU2017261820B2/en active Active
- 2017-05-12 US US15/594,487 patent/US20170327862A1/en not_active Abandoned
-
2018
- 2018-11-07 IL IL262847A patent/IL262847A/en unknown
Also Published As
Publication number | Publication date |
---|---|
RU2714841C1 (ru) | 2020-02-19 |
US20170327862A1 (en) | 2017-11-16 |
WO2017197364A1 (en) | 2017-11-16 |
AU2017261820A1 (en) | 2018-11-22 |
JP2019520804A (ja) | 2019-07-25 |
CN109415760A (zh) | 2019-03-01 |
BR112018073330A2 (pt) | 2019-02-26 |
EP3455373A1 (en) | 2019-03-20 |
RU2020106956A (ru) | 2020-08-13 |
CA3022847A1 (en) | 2017-11-16 |
KR20190003996A (ko) | 2019-01-10 |
AU2017261820B2 (en) | 2021-02-18 |
IL262847A (en) | 2018-12-31 |
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