MX2018004219A - Kit para prueba de elisa indirecto a base de hapteno nativo crudo y controles liofilizados para diagnostico confirmatorio de brucelosis bovina en suero sanguineo y leche por animal y de tanque. - Google Patents
Kit para prueba de elisa indirecto a base de hapteno nativo crudo y controles liofilizados para diagnostico confirmatorio de brucelosis bovina en suero sanguineo y leche por animal y de tanque.Info
- Publication number
- MX2018004219A MX2018004219A MX2018004219A MX2018004219A MX2018004219A MX 2018004219 A MX2018004219 A MX 2018004219A MX 2018004219 A MX2018004219 A MX 2018004219A MX 2018004219 A MX2018004219 A MX 2018004219A MX 2018004219 A MX2018004219 A MX 2018004219A
- Authority
- MX
- Mexico
- Prior art keywords
- controls
- indirect elisa
- milk
- animal
- tank
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/23—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Brucella (G)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Kit de diagnóstico para prueba confirmatoria por el método de ELISA Indirecto que mide los niveles de anticuerpos anti-Hapteno Nativo producidos en una verdadera infección, evitando grandes pérdidas económicas a la ganadería, con el discernimiento de ¨falsos positivos¨ los cuales presentan anticuerpos anti-LPS debido a reacciones cruzadas por enterobacterias y anticuerpos posvacunales en el diagnóstico de brucelosis bovina en suero sanguíneo y leche por animal y de tanque, caracterizado por que utiliza el antígeno Hapteno Nativo crudo, extraído de la cepa B. melitensis 16M, sin ningún tratamiento de purificación con efectiva capacidad de adherencia, que es empleado para la sensibilización o antigenización de las placas a una concentración conocida (1 µg por pozo), en donde tomando como referencia controles positivos y negativos sometidos al método de liofilización para garantizar su conservación, evitando la contaminación y la degradación de los anticuerpos presentes, y que garantiza la estabilidad de las densidades ópticas en dichos controles para una correcta interpretación de resultados en una ELISA indirecta. Adicionalmente, presenta el proceso de Liofilización de controles que pueden ser utilizados en otros métodos diagnósticos.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/MX2018/050009 WO2018186731A1 (es) | 2017-04-06 | 2018-04-05 | Kit para prueba de elisa indirecto a base de hapteno nativo crudo y controles liofilizados para diagnóstico confirmatorio de brucelosis bovina en suero sanguíneo y leche por animal y de tanque. |
| US16/603,019 US20210025888A1 (en) | 2017-04-06 | 2018-04-05 | Crude native hapten-based indirect elisa assay kit and lyophilised controls for the confirmatory diagnosis of bovine brucellosis in blood serum and milk by animal and tank |
| BR112019021004-0A BR112019021004A2 (pt) | 2017-04-06 | 2018-04-05 | kit e processos para teste de elisa indireto baseados no hapteno nativo bruto e no uso de controles liofilizados para diagnósticos confirmatórios de brucelose bovina em soro sanguíneo e no leite por animal e de tanque e processo de seleção e liofilização |
| CL2019002857A CL2019002857A1 (es) | 2017-04-06 | 2019-10-05 | Kit para prueba de elisa indirecto a base de hapteno nativo crudo y controles liofilizados para diagnóstico confirmatorio de brucelosis bovina en suero sanguíneo y leche por animal y de tanque. |
| CONC2019/0012373A CO2019012373A2 (es) | 2017-04-06 | 2019-11-05 | Kit para prueba de elisa indirecto a base de hapteno nativo crudo y controles liofilizados para diagnóstico confirmatorio de brucelosis bovina en suero sanguíneo y leche por animal y de tanque. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2017004490 | 2017-04-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2018004219A true MX2018004219A (es) | 2018-11-09 |
Family
ID=65023010
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2018004219A MX2018004219A (es) | 2017-04-06 | 2018-04-05 | Kit para prueba de elisa indirecto a base de hapteno nativo crudo y controles liofilizados para diagnostico confirmatorio de brucelosis bovina en suero sanguineo y leche por animal y de tanque. |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20210025888A1 (es) |
| EP (2) | EP4235178A3 (es) |
| BR (1) | BR112019021004A2 (es) |
| CL (1) | CL2019002857A1 (es) |
| CO (1) | CO2019012373A2 (es) |
| MX (1) | MX2018004219A (es) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113311156A (zh) * | 2021-04-09 | 2021-08-27 | 深圳市计量质量检测研究院（国家高新技术计量站、国家数字电子产品质量监督检验中心） | 一种牛乳蛋白和羊乳蛋白二联检测卡的制备方法及应用 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117187146B (zh) * | 2023-11-07 | 2024-01-26 | 中国兽医药品监察所 | 一株无耐药性的粗糙型布鲁氏菌病保护菌株及其应用 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MXNL06000077A (es) | 2006-10-25 | 2008-10-09 | Univ Autonoma De Nuevo Leon | Un antigeno modificado para deteccion de anticuerpos contra brucella y metodo de uso. |
-
2018
- 2018-04-05 EP EP23178992.6A patent/EP4235178A3/en active Pending
- 2018-04-05 MX MX2018004219A patent/MX2018004219A/es unknown
- 2018-04-05 EP EP18780939.7A patent/EP3608672B1/en active Active
- 2018-04-05 BR BR112019021004-0A patent/BR112019021004A2/pt not_active Application Discontinuation
- 2018-04-05 US US16/603,019 patent/US20210025888A1/en active Pending
-
2019
- 2019-10-05 CL CL2019002857A patent/CL2019002857A1/es unknown
- 2019-11-05 CO CONC2019/0012373A patent/CO2019012373A2/es unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113311156A (zh) * | 2021-04-09 | 2021-08-27 | 深圳市计量质量检测研究院（国家高新技术计量站、国家数字电子产品质量监督检验中心） | 一种牛乳蛋白和羊乳蛋白二联检测卡的制备方法及应用 |
| CN113311156B (zh) * | 2021-04-09 | 2022-11-29 | 深圳市计量质量检测研究院（国家高新技术计量站、国家数字电子产品质量监督检验中心） | 一种牛乳蛋白和羊乳蛋白二联检测卡的制备方法及应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3608672A1 (en) | 2020-02-12 |
| EP3608672A4 (en) | 2021-03-31 |
| EP3608672B1 (en) | 2023-10-25 |
| CL2019002857A1 (es) | 2020-05-08 |
| US20210025888A1 (en) | 2021-01-28 |
| EP4235178A2 (en) | 2023-08-30 |
| BR112019021004A2 (pt) | 2020-05-05 |
| CO2019012373A2 (es) | 2020-04-01 |
| EP4235178A3 (en) | 2023-10-18 |
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