MX2017004285A - Indicador biologico. - Google Patents

Indicador biologico.

Info

Publication number
MX2017004285A
MX2017004285A MX2017004285A MX2017004285A MX2017004285A MX 2017004285 A MX2017004285 A MX 2017004285A MX 2017004285 A MX2017004285 A MX 2017004285A MX 2017004285 A MX2017004285 A MX 2017004285A MX 2017004285 A MX2017004285 A MX 2017004285A
Authority
MX
Mexico
Prior art keywords
host organism
biological indicator
regulatory gene
insertion sequence
gene
Prior art date
Application number
MX2017004285A
Other languages
English (en)
Inventor
P Franciskovich Phillip
A Cregger Tricia
A Burke Peter
A Yirava William
Original Assignee
American Sterilizer Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Sterilizer Co filed Critical American Sterilizer Co
Publication of MX2017004285A publication Critical patent/MX2017004285A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/26—Accessories or devices or components used for biocidal treatment
    • A61L2/28—Devices for testing the effectiveness or completeness of sterilisation, e.g. indicators which change colour
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/06—Means for testing the completeness of the sterilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/22—Testing for sterility conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Esta invención se relaciona con un indicador biológico derivado de una composición que comprende. Un organismo hospedador que comprende una bacteria formadora de esporas; un gen marcador para producir una enzima indicadora; un gen regulador y un vehículo para insertar el gen marcador y el gen regulador en el organismo hospedador; el organismo hospedador presenta un elemento genético susceptible de transposición en su genoma para insertar una secuencia de inserción en el gen regulador; la secuencia de inserción comprende una transposasa, un par de secuencias repetidas invertidas terminales y por lo menos un marco de lectura abierto para expresar la transposasa. El vehículo puede ser captado por el organismo hospedador. La secuencia de inserción se puede insertar en el gen regulador. El organismo hospedador puede experimentar esporulación para formar el indicador biológico. Se describe un procedimiento y un aparato para utilizar el indicador biológico.
MX2017004285A 2014-10-13 2015-06-16 Indicador biologico. MX2017004285A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/512,708 US9416393B2 (en) 2014-10-13 2014-10-13 Biological indicator
PCT/US2015/035936 WO2016060714A1 (en) 2014-10-13 2015-06-16 Biological indicator

Publications (1)

Publication Number Publication Date
MX2017004285A true MX2017004285A (es) 2017-07-19

Family

ID=53783901

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017004285A MX2017004285A (es) 2014-10-13 2015-06-16 Indicador biologico.

Country Status (6)

Country Link
US (8) US9416393B2 (es)
EP (1) EP3206723A1 (es)
AU (1) AU2015334055B2 (es)
CA (1) CA2963508C (es)
MX (1) MX2017004285A (es)
WO (1) WO2016060714A1 (es)

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US10302614B2 (en) 2014-05-06 2019-05-28 Safetraces, Inc. DNA based bar code for improved food traceability
US9416393B2 (en) * 2014-10-13 2016-08-16 American Sterilizer Company Biological indicator
US10962512B2 (en) 2015-08-03 2021-03-30 Safetraces, Inc. Pathogen surrogates based on encapsulated tagged DNA for verification of sanitation and wash water systems for fresh produce
US20170211035A1 (en) * 2016-01-25 2017-07-27 American Sterilizer Company Biological indicators
US10287620B2 (en) 2016-04-29 2019-05-14 Bio-Rad Laboratories, Inc. Digital polymerase fidelity assay
WO2018071732A1 (en) * 2016-10-12 2018-04-19 Verrix, Llc Systems and methods for sterility assurance
CN106591419A (zh) * 2016-12-22 2017-04-26 南京巨鲨显示科技有限公司 一种新型基因工程生物指示剂
US20180245122A1 (en) * 2017-02-28 2018-08-30 American Sterilizer Company Recovery medium for detection of microorganisms by fluorescence
US10926264B2 (en) 2018-01-10 2021-02-23 Safetraces, Inc. Dispensing system for applying DNA taggants used in combinations to tag articles
GB201803437D0 (en) * 2018-03-02 2018-04-18 Secr Defence A disclosure system for detecting surface microorganism contamination
US10556032B2 (en) * 2018-04-25 2020-02-11 Safetraces, Inc. Sanitation monitoring system using pathogen surrogates and surrogate tracking
CN108998486A (zh) * 2018-08-02 2018-12-14 江南大学 一种木糖诱导型地衣芽孢杆菌产蛋白的诱导方法
CN108949896B (zh) * 2018-08-08 2021-10-29 北京瑞荧仪器科技有限公司 一种空气中微生物的荧光标记方法及其应用
US11200383B2 (en) 2018-08-28 2021-12-14 Safetraces, Inc. Product tracking and rating system using DNA tags
US11853832B2 (en) 2018-08-28 2023-12-26 Safetraces, Inc. Product tracking and rating system using DNA tags
EP3891163B1 (en) * 2018-12-06 2022-05-18 3M Innovative Properties Company Fluorinated 4' alkylumbelliferyl alpha-d-glucopyranosides, biological sterilization indicators including the same and methods of using the same
WO2020136462A1 (en) 2018-12-28 2020-07-02 Asp Global Manufacturing Gmbh A treatment indicator, a method of production thereof, and a method of use thereof
CA3148839A1 (en) 2019-07-26 2021-02-04 American Sterilizer Company Liquid sporulation method and sporulation broth
US11884960B2 (en) 2019-08-16 2024-01-30 Terragene Llc Biological indicator for determining the efficacy of a steam or heat sterilization process and its method of use
EP4034544B1 (en) * 2019-09-25 2023-11-15 3M Innovative Properties Company 8-fluoro-4-alkylumbelliferyl alpha-d-glucopyranoside, biological sterilization indicator including the same and its use in a method of determining efficacy of a sterilization process
US11603551B2 (en) 2020-12-02 2023-03-14 Steritec Products Mfg. Co., Inc. Biological indicators, and systems and methods for determining efficacy of sterilization
EP4373830A1 (en) * 2021-07-22 2024-05-29 Solventum Intellectual Properties Company Chlorinated 4'-alkylumbelliferyl alpha-d-glucopyranoside and method of assessing efficacy of a sterilization process including the same
WO2023021348A1 (en) * 2021-08-18 2023-02-23 3M Innovative Properties Company Device, system, and method for determining the efficacy of a sterilization process
EP4448756A1 (en) 2021-12-16 2024-10-23 Danmarks Tekniske Universitet Temperature-inducible expression system for thermophilic organisms
CN116621897B (zh) * 2023-05-08 2024-07-09 中国药科大学 东莨菪苷衍生物及其制备方法和医药用途

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US8895239B2 (en) 2006-09-20 2014-11-25 American Sterilizer Company Genetically engineered biological indicator
JP6178239B2 (ja) 2010-11-01 2017-08-09 スリーエム イノベイティブ プロパティズ カンパニー 生物活性の検出方法
BR112013008703B1 (pt) 2010-11-01 2020-10-27 3M Innovative Properties Company método e sistema de indicador biológico de esterilização
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US9416393B2 (en) * 2014-10-13 2016-08-16 American Sterilizer Company Biological indicator

Also Published As

Publication number Publication date
US20160102335A1 (en) 2016-04-14
US9701968B2 (en) 2017-07-11
WO2016060714A1 (en) 2016-04-21
US10017772B2 (en) 2018-07-10
EP3206723A1 (en) 2017-08-23
AU2015334055B2 (en) 2019-09-19
US20160312263A1 (en) 2016-10-27
CA2963508A1 (en) 2016-04-21
US10301632B2 (en) 2019-05-28
CA2963508C (en) 2021-11-09
US20160312232A1 (en) 2016-10-27
US20170275633A1 (en) 2017-09-28
US10011843B2 (en) 2018-07-03
US9416393B2 (en) 2016-08-16
US20170275631A1 (en) 2017-09-28
US10301631B2 (en) 2019-05-28
US20180273958A1 (en) 2018-09-27
US10011844B2 (en) 2018-07-03
US9695428B2 (en) 2017-07-04
AU2015334055A1 (en) 2017-04-13
US20170275632A1 (en) 2017-09-28
US20180273957A1 (en) 2018-09-27

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