MX2016008371A - Metodo y kit de deteccion de acido nucleico. - Google Patents

Metodo y kit de deteccion de acido nucleico.

Info

Publication number
MX2016008371A
MX2016008371A MX2016008371A MX2016008371A MX2016008371A MX 2016008371 A MX2016008371 A MX 2016008371A MX 2016008371 A MX2016008371 A MX 2016008371A MX 2016008371 A MX2016008371 A MX 2016008371A MX 2016008371 A MX2016008371 A MX 2016008371A
Authority
MX
Mexico
Prior art keywords
nucleic acid
kit
target nucleic
detection method
detected
Prior art date
Application number
MX2016008371A
Other languages
English (en)
Inventor
Trau Matt
WEE Eugene
Ramon Botella Jose
Original Assignee
Univ Queensland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2013905052A external-priority patent/AU2013905052A0/en
Application filed by Univ Queensland filed Critical Univ Queensland
Publication of MX2016008371A publication Critical patent/MX2016008371A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proporcionan un método y kit para detectar un ácido nucleico objetivo en donde la combinación de un ácido nucleico amplificado con una partícula tal como un glóbulo paramagnético forma un complejo floculado que se puede detectar mediante inspección visual. El volumen de la muestra de ácido nucleico que se puede detectar es tan bajo como unos cuantos microlitros. El método se puede aplicar para la diagnosis en el campo o en el punto de cuidado para la determinación rápida de la presencia o ausencia del ácido nucleico objetivo. El estado de metilación de un ácido nucleico objetivo también se puede determinar. El método y el kit pueden tener aplicabilidad general para la detección de enfermedades en plantas y animales, la prueba ambiental y la prueba para contaminación de alimentos y otros productos comestibles.
MX2016008371A 2013-12-23 2014-12-23 Metodo y kit de deteccion de acido nucleico. MX2016008371A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2013905052A AU2013905052A0 (en) 2013-12-23 Nucleic acid detection method and kit
PCT/AU2014/050443 WO2015095929A1 (en) 2013-12-23 2014-12-23 Nucleic acid detection method and kit

Publications (1)

Publication Number Publication Date
MX2016008371A true MX2016008371A (es) 2016-10-14

Family

ID=53477221

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016008371A MX2016008371A (es) 2013-12-23 2014-12-23 Metodo y kit de deteccion de acido nucleico.

Country Status (9)

Country Link
US (1) US10465236B2 (es)
EP (1) EP3087202B1 (es)
JP (1) JP2017501719A (es)
CN (1) CN106062210A (es)
AU (1) AU2014373622B2 (es)
BR (1) BR112016014695A2 (es)
CA (1) CA2934641A1 (es)
MX (1) MX2016008371A (es)
WO (1) WO2015095929A1 (es)

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CN105296670B (zh) * 2015-11-09 2018-10-19 山西省农业科学院畜牧兽医研究所 一种h1、h3和h9型禽流感病毒检测试剂盒及检测方法
US20210254186A1 (en) * 2016-08-08 2021-08-19 The Secretary Of State For Health Flavivirus diagnostic array
CN106929584A (zh) * 2017-03-30 2017-07-07 上海市农业科学院 一种检测Camv35S启动子的RPA引物、试剂盒及检测方法
US11746372B2 (en) 2017-12-01 2023-09-05 Godx, Inc. Rapid nucleic acids separation and sample preparation via hollow-centered silica microsphere
CN108265050A (zh) * 2018-03-13 2018-07-10 普迈德（北京）科技有限公司 一种血浆直接亚硫酸氢盐转化的方法及其应用
US20220042090A1 (en) * 2018-09-14 2022-02-10 Cold Spring Harbor Laboratory PROGRAMMABLE RNA-TEMPLATED SEQUENCING BY LIGATION (rSBL)
CN109765371A (zh) * 2019-02-19 2019-05-17 吉林省爱诺德生物工程有限公司 一种快速检测恶性肿瘤dna的液相纳米胶体金比色方法
CN110241021B (zh) * 2019-05-23 2022-04-12 广州普世利华科技有限公司 一种免仪器核酸现场快速检测试剂盒
JP7133867B2 (ja) * 2019-09-25 2022-09-09 国立大学法人九州大学 核酸の検知方法及び核酸の検知装置
US20210324371A1 (en) * 2020-04-16 2021-10-21 Noblis, Inc. Portable field-deployable nucleic acid sequencing kit
CN113278736B (zh) * 2021-05-28 2023-05-05 深圳市爱医生物科技有限公司 用于定性检测牛疱疹病毒i型的试剂和方法
ES1298031Y (es) * 2022-02-11 2023-06-02 Consorcio De Aguas De Asturias Sistema de analisis de aguas residuales o ambientales para la deteccion de virus, bacterias o microorganismos eucariotas
WO2024047567A1 (en) 2022-08-31 2024-03-07 VD-ING d.o.o. Method, device and system for detecting microbial or viral infection
CN115820852B (zh) * 2022-09-15 2023-09-01 广州医科大学附属第一医院（广州呼吸中心） 一种用于检测tmprss2-erg融合基因的电化学传感器及其应用
WO2024064704A1 (en) * 2022-09-19 2024-03-28 Rt Microdx Inc. Pathogen testing device and method
NL2033124B1 (en) 2022-09-23 2024-03-29 Rapidemic B V Methods and device for multiple-label nucleic acid amplification and detection

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0259453A1 (en) 1986-03-04 1988-03-16 Cambridge Biotech Corporation Nucleic acid detection using particle agglutination
US5989813A (en) 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
ATE231875T1 (de) * 1996-03-20 2003-02-15 Bio Merieux Isolierung von nukleinsäure
US6096563A (en) * 1996-03-29 2000-08-01 Strategic Diagnostics Inc. Dual particle immunoassay method and kit
EP1478774A4 (en) * 2001-10-09 2006-11-29 Nanosphere Inc ELECTRONIC DETECTION OF DNA BASED ON AN ASSEMBLY AND IMPLEMENTING NANOPARTICLE-LIKE PROBES
WO2004111602A2 (en) * 2003-05-16 2004-12-23 University Of Rochester Colorimetric and fluorescent methods for sensing of oligonucleotides
WO2011150226A1 (en) 2010-05-26 2011-12-01 Landers James P Method for detecting nucleic acids based on aggregate formation
US20130115616A1 (en) 2010-07-15 2013-05-09 Hitachi Chemical Research Center, Inc. Detection of nucleic acids by agglutination
US20120108451A1 (en) 2010-10-29 2012-05-03 Simon Fraser University Methods and apparatus for nanoparticle-assisted nucleic acid hybridization and microarray analysis
WO2012151268A1 (en) * 2011-05-02 2012-11-08 University Of Virginia Patent Foundation Method and system for high throughput optical and label free detection of analytes
WO2014065753A1 (en) * 2012-10-23 2014-05-01 Dso National Laboratories Direct visual detection of nucleic acid using gold nanoparticles

Also Published As

Publication number Publication date
US20170029881A1 (en) 2017-02-02
US10465236B2 (en) 2019-11-05
EP3087202B1 (en) 2020-10-14
CA2934641A1 (en) 2015-07-02
AU2014373622A1 (en) 2016-07-28
WO2015095929A1 (en) 2015-07-02
JP2017501719A (ja) 2017-01-19
CN106062210A (zh) 2016-10-26
AU2014373622B2 (en) 2021-05-27
EP3087202A1 (en) 2016-11-02
BR112016014695A2 (pt) 2017-08-08
EP3087202A4 (en) 2017-07-05

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