MX2016008103A - Proceso para producir un polímero beta-glucano y los microorganismos genéticamente útiles en este proceso. - Google Patents

Proceso para producir un polímero beta-glucano y los microorganismos genéticamente útiles en este proceso.

Info

Publication number
MX2016008103A
MX2016008103A MX2016008103A MX2016008103A MX2016008103A MX 2016008103 A MX2016008103 A MX 2016008103A MX 2016008103 A MX2016008103 A MX 2016008103A MX 2016008103 A MX2016008103 A MX 2016008103A MX 2016008103 A MX2016008103 A MX 2016008103A
Authority
MX
Mexico
Prior art keywords
beta
producing
main chain
polymer
linear main
Prior art date
Application number
MX2016008103A
Other languages
English (en)
Inventor
Brockmann Beata
Herold Andrea
Fleck Christian
Granström Mari
Hlubek Andrea
Original Assignee
Wintershall Holding GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wintershall Holding GmbH filed Critical Wintershall Holding GmbH
Publication of MX2016008103A publication Critical patent/MX2016008103A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01018—1,4-Alpha-glucan branching enzyme (2.4.1.18), i.e. glucan branching enzyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01001—Hexokinase (2.7.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07009—UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02002—Phosphoglucomutase (5.4.2.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Proceso para producir un polímero que consiste en una cadena principal lineal de unidades beta-D-(1-3)-glucopiranosilo que tienen una unidad de beta-D-glucopiranosilo (1-6) única unida a una unidad de beta-D-glucopiranosilo de la cadena principal lineal con un grado de ramificación promedio de aproximadamente 0,3, dicho proceso que comprende las etapas de: (i) cultivar en un medio un microorganismo modificado genéticamente capaz de producir un polímero que consiste en una cadena principal lineal de unidades de beta-D-(1-3)-glucopiranosilo que tienen una unidad de beta-D-glucopiranosilo (1-6) única unidad a una unidad de beta-D-glucopiranosilo de la cadena principal lineal con un grado de ramificación promedio de aproximadamente 0,3, donde la modificación confiere un aumento de actividad - en comparación con un microorganismo de control no modificado de la misma cepa - , de al menos dos productos génicos seleccionados del grupo que consiste en (a) hexoquinasa, (b) fosfoglucomutasa,. (c) UTP-glucosa-1-fosfato uridiltrasferasa, (d) 1,3-beta-glucanosintas a (e) enzima de ramificación; en condiciones que permiten que dicho microorganismo produzca dicho polímero; (ii) recuperar en forma opcional, dicho polímero del medio.
MX2016008103A 2013-12-19 2014-12-12 Proceso para producir un polímero beta-glucano y los microorganismos genéticamente útiles en este proceso. MX2016008103A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13198610 2013-12-19
PCT/EP2014/077479 WO2015091231A1 (en) 2013-12-19 2014-12-12 Process for producing a beta-glucan polymer and genetically modified microorganisms useful in this process

Publications (1)

Publication Number Publication Date
MX2016008103A true MX2016008103A (es) 2016-10-03

Family

ID=49911255

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016008103A MX2016008103A (es) 2013-12-19 2014-12-12 Proceso para producir un polímero beta-glucano y los microorganismos genéticamente útiles en este proceso.

Country Status (9)

Country Link
EP (1) EP3083951B1 (es)
KR (1) KR102263796B1 (es)
CN (1) CN106029878B (es)
BR (1) BR112016013850B1 (es)
CA (1) CA2933955A1 (es)
MX (1) MX2016008103A (es)
RU (1) RU2016129208A (es)
WO (1) WO2015091231A1 (es)
ZA (1) ZA201604767B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109609525A (zh) * 2019-01-10 2019-04-12 江苏大学 灰树花葡聚糖合成酶、其编码基因及应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100892355B1 (ko) * 2008-01-07 2009-04-08 (주)큐젠바이오텍 치마버섯 균사체의 반연속식 액상배양을 통한 베타-1,6-분지-베타-1,3-글루칸 대량생산 방법

Also Published As

Publication number Publication date
CA2933955A1 (en) 2015-06-25
KR20160103037A (ko) 2016-08-31
EP3083951A1 (en) 2016-10-26
ZA201604767B (en) 2017-09-27
CN106029878B (zh) 2021-06-29
EP3083951B1 (en) 2021-10-06
BR112016013850A2 (pt) 2018-01-09
BR112016013850B1 (pt) 2023-02-07
WO2015091231A1 (en) 2015-06-25
CN106029878A (zh) 2016-10-12
RU2016129208A (ru) 2018-01-24
KR102263796B1 (ko) 2021-06-10

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