MX2012009538A - Metodo para la determinacion de variantes de secuencia de polipeptido. - Google Patents

Metodo para la determinacion de variantes de secuencia de polipeptido.

Info

Publication number
MX2012009538A
MX2012009538A MX2012009538A MX2012009538A MX2012009538A MX 2012009538 A MX2012009538 A MX 2012009538A MX 2012009538 A MX2012009538 A MX 2012009538A MX 2012009538 A MX2012009538 A MX 2012009538A MX 2012009538 A MX2012009538 A MX 2012009538A
Authority
MX
Mexico
Prior art keywords
amino acid
acid sequence
sample
evaluation
data
Prior art date
Application number
MX2012009538A
Other languages
English (en)
Inventor
Hans Koll
Joerg Thomas Regula
Anne Zeck
Frank Wiegeshoff
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2012009538A publication Critical patent/MX2012009538A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La presente invención está dirigida a un método para. determinare mutaciones de una secuencia de aminoácido en un polipéptido producido, que comprende los siguientes pasaos de a) proporcionar una muestra de un polipéptido producido, b) incubar el polipéptido en la muestra con una proteasa, c) llevar a cabo un análisis bidimensional utilizando cromatografía de fase inversa acoplada con una espectroscopía de masas de alta resolución (FT-ICR/FT-orbitrap) y análisis Ms/MS de los fragmentos de la secuencia de aminoácido de los péptidos, d) evaluación de los datos comparando los grupos de datos LC-MS obtenidos para las muestras paralelamente con el grupo de datos de un muestra de referencia, mediante la búsqueda de diferencias en las intensidades de señal a tiempos de retención dados y por la evaluación de las señales diferenciales con respecto a las mutaciones de la secuencia de aminoácido. La muestra de referencia para la evaluación de los datos (d) puede ser ya sea un estándar bien caracterizado o una de las muestras a ser analizada.
MX2012009538A 2010-02-18 2011-02-16 Metodo para la determinacion de variantes de secuencia de polipeptido. MX2012009538A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10001645 2010-02-18
PCT/EP2011/052282 WO2011101370A1 (en) 2010-02-18 2011-02-16 Method for the determination of sequence variants of polypeptides

Publications (1)

Publication Number Publication Date
MX2012009538A true MX2012009538A (es) 2012-08-31

Family

ID=42224873

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012009538A MX2012009538A (es) 2010-02-18 2011-02-16 Metodo para la determinacion de variantes de secuencia de polipeptido.

Country Status (12)

Country Link
US (3) US20120322093A1 (es)
EP (1) EP2537035B1 (es)
JP (1) JP5903051B2 (es)
KR (1) KR20120120500A (es)
CN (1) CN102762987B (es)
BR (1) BR112012017532A2 (es)
CA (1) CA2787898A1 (es)
ES (1) ES2609669T3 (es)
MX (1) MX2012009538A (es)
RU (1) RU2012138298A (es)
SG (2) SG183320A1 (es)
WO (1) WO2011101370A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106574914B (zh) * 2014-06-24 2018-09-11 株式会社岛津制作所 全二维色谱用数据处理装置
US10781484B1 (en) 2015-09-30 2020-09-22 Agilent Technologies, Inc. Method of oligonucleotide analysis
JP2020505931A (ja) * 2017-02-03 2020-02-27 ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ 生物学的製品の製造において複数の細胞を分析しタンパク質配列変異体を検出する方法
US20210095348A1 (en) * 2017-09-06 2021-04-01 Lawrence Livermore National Security, Llc Methods and systems to perform genetically variant protein analysis, and related marker genetic protein variations and databases
JP7135561B2 (ja) * 2018-08-08 2022-09-13 株式会社島津製作所 分析制御装置、分析システムおよび分析方法
JP6996451B2 (ja) * 2018-08-24 2022-01-17 株式会社島津製作所 分析支援装置および分析支援方法
US11726096B2 (en) * 2018-10-04 2023-08-15 Regeneron Pharmaceuticals, Inc. Fast protein sequencing
CN113406336B (zh) * 2021-06-15 2024-06-07 上海臻复医疗科技有限公司 一种适用于质谱鉴定的毛干蛋白质提取方法及其应用
US20230243843A1 (en) * 2022-01-10 2023-08-03 Regeneron Pharmaceuticals, Inc. Sequence variance analysis by proteominer

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2114168A1 (en) * 1993-03-05 1994-09-06 Zlatko Dembic Chimeric human interferon-gamma-receptor/immunoglobulin polypeptides
US7158862B2 (en) * 2000-06-12 2007-01-02 The Arizona Board Of Regents On Behalf Of The University Of Arizona Method and system for mining mass spectral data
CN1376692A (zh) * 2001-03-22 2002-10-30 上海博德基因开发有限公司 一种多肽——免疫球蛋白轻链κ1蛋白－26.07和编码这种多肽的多核苷酸
WO2003042774A2 (en) * 2001-11-13 2003-05-22 Caprion Pharmaceuticals Inc. Mass intensity profiling system and uses thereof
AU2002218321A1 (en) * 2001-11-30 2003-06-10 The European Molecular Biology Laboratory A system and method for automatic protein sequencing by mass spectrometry
AU2003298733B2 (en) * 2002-11-27 2009-06-18 Agena Bioscience, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
CN100383244C (zh) * 2003-01-07 2008-04-23 西福根有限公司 用于生产重组多克隆蛋白质的方法
GB0308202D0 (en) * 2003-04-09 2003-05-14 King S College London A screening method using mass spectrometry
ATE510025T1 (de) * 2005-12-13 2011-06-15 Inst Curie Verfahren für die detektion von mutationen in nukleinsaüren, und die anwendung in der diagnose von genetischen krankheiten und krebs

Also Published As

Publication number Publication date
RU2012138298A (ru) 2014-03-27
KR20120120500A (ko) 2012-11-01
SG10201501080VA (en) 2015-04-29
EP2537035A1 (en) 2012-12-26
BR112012017532A2 (pt) 2019-09-24
US20120322093A1 (en) 2012-12-20
CN102762987A (zh) 2012-10-31
ES2609669T3 (es) 2017-04-21
CA2787898A1 (en) 2011-08-25
WO2011101370A1 (en) 2011-08-25
EP2537035B1 (en) 2016-11-16
JP2013519099A (ja) 2013-05-23
JP5903051B2 (ja) 2016-04-13
SG183320A1 (en) 2012-09-27
CN102762987B (zh) 2016-03-30
US20210371897A1 (en) 2021-12-02
US20150064735A1 (en) 2015-03-05

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