MX2011011040A - Grupo de sondas para identificar la mutacion del nucleotido y metodo para identificar la mutacion del nucleotido. - Google Patents

Grupo de sondas para identificar la mutacion del nucleotido y metodo para identificar la mutacion del nucleotido.

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Publication number
MX2011011040A
MX2011011040A MX2011011040A MX2011011040A MX2011011040A MX 2011011040 A MX2011011040 A MX 2011011040A MX 2011011040 A MX2011011040 A MX 2011011040A MX 2011011040 A MX2011011040 A MX 2011011040A MX 2011011040 A MX2011011040 A MX 2011011040A
Authority
MX
Mexico
Prior art keywords
nucleotide
nucleotide sequence
probe
mutation site
identification
Prior art date
Application number
MX2011011040A
Other languages
English (en)
Inventor
Yasuhiro Kishi
Naohiro Kamiya
Original Assignee
Vertex Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vertex Pharma filed Critical Vertex Pharma
Publication of MX2011011040A publication Critical patent/MX2011011040A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21098—Hepacivirin (3.4.21.98)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Communicable Diseases (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

El tipo de un nucleótido localizado en un sitio de mutación en un ácido nucleico objetivo puede identificarse llevando a cabo una PCR en tiempo real fluorescente utilizando un grupo de sondas que comprende una sonda de detección que comprende un oligonucleótido que comprende una secuencia de nucleótido que contiene el sitio de mutación en el ácido nucleico objetivo y también contiene un nucleótido de interés en el sitio de mutación o una secuencia de nucleótido complementaria a la secuencia de nucleótido antes mencionada, tiene una sustancia fluorescente adicionada en el en 5´- terminal y una sustancia extintora adicionada en 3´-terminal, y tiene, introducida en la misma, una modificación en donde la temperatura de fusión de la sonda se convierte en 3°C o más alta que la de la sonda contadora, y en donde la sonda contadora comprende un oligonucleótido que comprende una secuencia de nucleótido que contiene un sitio de mutación y también contiene un nucleótido que es diferente del nucleótido de interés en el sitio de mutación o una secuencia de nucleótido complementaria a la secuencia de nucleótido antes mencionada.
MX2011011040A 2009-04-22 2010-04-22 Grupo de sondas para identificar la mutacion del nucleotido y metodo para identificar la mutacion del nucleotido. MX2011011040A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2009104468 2009-04-22
PCT/JP2010/057135 WO2010123058A1 (ja) 2009-04-22 2010-04-22 塩基変異判別用プローブセットおよび塩基変異の判別方法

Publications (1)

Publication Number Publication Date
MX2011011040A true MX2011011040A (es) 2011-11-04

Family

ID=43011174

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2011011040A MX2011011040A (es) 2009-04-22 2010-04-22 Grupo de sondas para identificar la mutacion del nucleotido y metodo para identificar la mutacion del nucleotido.

Country Status (13)

Country Link
US (1) US20120095116A1 (es)
EP (1) EP2423324A4 (es)
JP (1) JPWO2010123058A1 (es)
KR (1) KR20110138289A (es)
CN (1) CN102459640A (es)
AU (1) AU2010240092A1 (es)
CA (1) CA2759443A1 (es)
IL (1) IL215862A0 (es)
MX (1) MX2011011040A (es)
NZ (1) NZ596528A (es)
RU (1) RU2011147195A (es)
WO (1) WO2010123058A1 (es)
ZA (1) ZA201107977B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9078747B2 (en) 2011-12-21 2015-07-14 Edwards Lifesciences Corporation Anchoring device for replacing or repairing a heart valve
EP2867372B1 (en) 2012-06-27 2018-12-19 Mobidiag Oy Method for determining the presence of diarrhoea causing pathogens
WO2017212023A1 (en) * 2016-06-10 2017-12-14 Roche Diagnostics Gmbh Compositions and methods for detection of hepatitis c virus genotype 3
EP3697927B1 (en) * 2017-10-19 2022-12-14 Bio-Rad Laboratories, Inc. Digital amplification assays with unconventional and/or inverse changes in photoluminescence
CN108949929A (zh) * 2018-08-28 2018-12-07 生工生物工程（上海）股份有限公司 用于同时检测mthfr和mtrr基因多态性的产品及其方法和应用
EP3877550A4 (en) * 2018-11-09 2022-08-10 Longhorn Vaccines and Diagnostics, LLC RAPID PCR METHODOLOGY
CN110157703A (zh) * 2019-05-21 2019-08-23 珠海圣美生物诊断技术有限公司 一种用于扩增变异型靶基因片段的非淬灭型寡核苷酸探针及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP2008523828A (ja) * 2004-12-22 2008-07-10 エクシコン・アクティーゼルスカブ 核酸混合物を解析するためのプローブ、ライブラリーおよびキット、並びにその構築方法
JP2008306974A (ja) * 2007-06-14 2008-12-25 Saitama Medical Univ Ｈｃｖ遺伝子型判定方法、並びに、これに用いるｌｎａプローブ及びキット
CN101338343B (zh) * 2007-07-03 2011-08-31 英科新创(厦门)科技有限公司 检测乙型肝炎病毒dna及其g1896a突变的方法及试剂盒

Also Published As

Publication number Publication date
RU2011147195A (ru) 2013-05-27
AU2010240092A1 (en) 2011-12-08
ZA201107977B (en) 2013-01-30
CN102459640A (zh) 2012-05-16
US20120095116A1 (en) 2012-04-19
KR20110138289A (ko) 2011-12-26
IL215862A0 (en) 2012-01-31
JPWO2010123058A1 (ja) 2012-10-25
EP2423324A1 (en) 2012-02-29
NZ596528A (en) 2014-04-30
CA2759443A1 (en) 2010-10-28
EP2423324A4 (en) 2013-02-13
WO2010123058A1 (ja) 2010-10-28

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