MX2009002214A - Metodos para separacion de celulas de esperma y composiciones que contienen aptameros o secuencias de acido nucleico para uso en ellos. - Google Patents

Metodos para separacion de celulas de esperma y composiciones que contienen aptameros o secuencias de acido nucleico para uso en ellos.

Info

Publication number
MX2009002214A
MX2009002214A MX2009002214A MX2009002214A MX2009002214A MX 2009002214 A MX2009002214 A MX 2009002214A MX 2009002214 A MX2009002214 A MX 2009002214A MX 2009002214 A MX2009002214 A MX 2009002214A MX 2009002214 A MX2009002214 A MX 2009002214A
Authority
MX
Mexico
Prior art keywords
nucleic acid
aptamers
sperm cell
acid molecules
target molecule
Prior art date
Application number
MX2009002214A
Other languages
English (en)
Inventor
David A Oksenberg
Sergey Krylov
Michael Musheev
Original Assignee
Biocern Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocern Inc filed Critical Biocern Inc
Publication of MX2009002214A publication Critical patent/MX2009002214A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Reproductive Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La presente invención provee un aptámero o un grupo de aptámeros que se une(n) a una molécula de blanco en la superficie de una célula de esperma de mamífero, y a un método para producir los aptámeros. El método comprende poner en contacto una colección de diferentes moléculas de ácido nucleico con la molécula de blanco, bajo condiciones favorables para la unión entre al menos una de las moléculas de ácido nucleico y la molécula de blanco, para formar por lo menos un complejo que comprende la unión de la molécula de ácido nucleico a la molécula de blanco; donde cada una de las moléculas de ácido nucleico comprende por lo menos un segmento de secuencias de nucleótido seleccionadas al azar. Se separan luego los complejos de las moléculas de ácido nucleico sin unir y las moléculas de blanco sin unir; y se recupera la molécula de ácido nucleico unida, del complejo separado. Se usan los aptámeros para separar células de esperma con base en las calidades de las células de esperma, por ejemplo, si las células contienen cromosomas X o cromosomas Y.
MX2009002214A 2006-08-30 2007-08-30 Metodos para separacion de celulas de esperma y composiciones que contienen aptameros o secuencias de acido nucleico para uso en ellos. MX2009002214A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82406906P 2006-08-30 2006-08-30
PCT/US2007/077300 WO2008028081A2 (en) 2006-08-30 2007-08-30 Sperm cell separation methods and compositions containing aptamers or nucleic acid sequences for use therein

Publications (1)

Publication Number Publication Date
MX2009002214A true MX2009002214A (es) 2009-07-02

Family

ID=39136915

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009002214A MX2009002214A (es) 2006-08-30 2007-08-30 Metodos para separacion de celulas de esperma y composiciones que contienen aptameros o secuencias de acido nucleico para uso en ellos.

Country Status (11)

Country Link
US (2) US8138319B2 (es)
EP (1) EP2061478A4 (es)
CN (1) CN101553237B (es)
AU (1) AU2007289164A1 (es)
BR (1) BRPI0714897A2 (es)
CA (1) CA2697960A1 (es)
CO (1) CO6190565A2 (es)
MX (1) MX2009002214A (es)
NZ (1) NZ575829A (es)
RU (1) RU2475539C2 (es)
WO (1) WO2008028081A2 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006091717A1 (en) * 2005-02-24 2006-08-31 Biocern, Inc. Sperm cell separation methods and compositions
CN101553237B (zh) 2006-08-30 2015-05-27 比奥塞尔恩公司 精细胞分离方法和含有其中所用适体或核酸序列的组合物
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US20110136099A1 (en) 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
EP2229457A4 (en) 2007-12-14 2011-01-26 Minitube America Inc SEX-SPECIFIC SEPARATION OF SPERM AND EMBRYOES
EP2316935B1 (en) * 2008-07-14 2016-08-31 The University of Tokyo Aptamer against il-17 and use thereof
US8703416B2 (en) 2008-07-17 2014-04-22 Somalogic, Inc. Method for purification and identification of sperm cells
CA2793945A1 (en) * 2009-04-01 2011-10-21 Biocern, Inc. Sperm cell separation methods and compositions containing sperm cell targeting ligands for use therein
GB0910826D0 (en) 2009-06-23 2009-08-05 Ovasort Ltd Identification of sex-linked proteins
WO2012174496A2 (en) * 2011-06-17 2012-12-20 Somalogic, Inc. Method for purification and identification of sperm cells
CN102796730A (zh) * 2012-07-18 2012-11-28 海南广陵高科实业有限公司 一种从聚丙烯酰胺凝胶中回收核酸分子的方法
WO2014035840A1 (en) 2012-08-29 2014-03-06 Krug Kristie Marie Magnetic removal or identification of damaged or compromised cells or cellular structures
US10379026B2 (en) 2012-08-29 2019-08-13 Inguran, Llc Cell processing using magnetic particles
RU2528870C2 (ru) * 2012-11-06 2014-09-20 Государственное бюджетное образовательное учреждение высшего профессионального образования "Красноярский государственный медицинский университет имени профессора В.Ф. Войно-Ясенецкого" Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО КрасГМУ им. проф. В.Ф. Войно-Ясе Аптамер, специфичный к опухолевым тканям легкого человека
EP2977454B1 (en) 2013-03-22 2018-05-02 The University of Tokyo Aptamer to il-17 and use thereof
CN105188933B (zh) * 2013-05-03 2017-09-19 莫迪利蒂康特公司 用于分析细胞运动性的设备
DE102013218528B3 (de) 2013-09-16 2014-12-24 ViaMed GmbH Vorrichtung zur Anreicherung von Spermatozoen
US11008575B2 (en) * 2015-07-28 2021-05-18 The University Of North Carolina At Charlotte DNA aptamers against cancer and uses thereof in delivery of therapy and diagnosis of cancer
CN110607303A (zh) * 2019-10-30 2019-12-24 多能干细胞再生医学科技（广州）有限公司 用于分离精子细胞群的核酸适体及方法
CN111876367A (zh) * 2020-07-10 2020-11-03 华侨大学 一种去除低质精子的方法

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CN101553237B (zh) 2006-08-30 2015-05-27 比奥塞尔恩公司 精细胞分离方法和含有其中所用适体或核酸序列的组合物

Also Published As

Publication number Publication date
CO6190565A2 (es) 2010-08-19
CA2697960A1 (en) 2008-03-06
EP2061478A4 (en) 2010-07-07
BRPI0714897A2 (pt) 2013-10-29
WO2008028081A3 (en) 2008-06-12
NZ575829A (en) 2012-04-27
AU2007289164A1 (en) 2008-03-06
WO2008028081A2 (en) 2008-03-06
US9546397B2 (en) 2017-01-17
US20090306461A1 (en) 2009-12-10
CN101553237A (zh) 2009-10-07
US20150203811A1 (en) 2015-07-23
EP2061478A2 (en) 2009-05-27
RU2475539C2 (ru) 2013-02-20
CN101553237B (zh) 2015-05-27
RU2009111277A (ru) 2010-10-10
US8138319B2 (en) 2012-03-20

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