MX178723A - Microorganism culture procedure in a solid medium consisting of an understandable and non-fermentable solid absorbent support - Google Patents

Microorganism culture procedure in a solid medium consisting of an understandable and non-fermentable solid absorbent support

Info

Publication number
MX178723A
MX178723A MX2076790A MX2076790A MX178723A MX 178723 A MX178723 A MX 178723A MX 2076790 A MX2076790 A MX 2076790A MX 2076790 A MX2076790 A MX 2076790A MX 178723 A MX178723 A MX 178723A
Authority
MX
Mexico
Prior art keywords
solid
support
medium consisting
understandable
fermentable
Prior art date
Application number
MX2076790A
Other languages
Spanish (es)
Inventor
Maurice Raimbault
Sevastianos Roussos
Eric Oriol
Gustavo Viniegra
Mariano Gutierrez
Javier Barrios-Gonzalez
Original Assignee
Orstom
Univ Autonoma Metropolita
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orstom, Univ Autonoma Metropolita filed Critical Orstom
Publication of MX178723A publication Critical patent/MX178723A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P27/00—Preparation of compounds containing a gibbane ring system, e.g. gibberellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a: Un procedimiento de cultivo de microorganismos de origen fúngico, (preferiblemente de los géneros Aspergillus, Penicillium, Rhizopus, Trichoderma, Geotrichum, Fusarium, Mucor y Giberella), sobre un medio de cultivo sólido constitutido de un soporte solido y un líquido conteniendo los nutrientes necesarios para el cultivo, caracterizado por in cluir las siguientes etapas: a) Trituración en partículas del soporte, humedecimiento del mismo con la solución acuosa conteniendo ciertos elementos de la solución nutritiva y esterilización del mismo; b) Impregnación del soporte asi tratado, con la solución nutritiva que contiene el o los substratos carbonados solubles, los agentes de crecimiento y el material inoculado hasta una humedad del conjunto de entre 50 y 85 por ciento c) Fermentación del medio; d) Extracción del 70 por ciento de la fracción acuosa que contiene los elementos biosintetizados (metabolitos secundarios y/o enzimas), mediante la compresión del citado soporte sólido.The present invention relates to: A method of cultivation of microorganisms of fungal origin, (preferably from the genera Aspergillus, Penicillium, Rhizopus, Trichoderma, Geotrichum, Fusarium, Mucor and Giberella), on a solid culture medium consisting of a solid support and a liquid containing the necessary nutrients for the culture, characterized by including the following stages: a) Crushing the support into particles, moistening it with the aqueous solution containing certain elements of the nutrient solution and sterilizing it; b) Impregnation of the support thus treated, with the nutrient solution containing the soluble carbon substratum or substrates, the growth agents and the inoculated material up to a humidity of between 50 and 85 percent as a whole. c) Fermentation of the medium; d) Extraction of 70 percent of the aqueous fraction containing the biosynthesized elements (secondary metabolites and / or enzymes), by compressing said solid support.

MX2076790A 1989-05-19 1990-03-18 Microorganism culture procedure in a solid medium consisting of an understandable and non-fermentable solid absorbent support MX178723A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8906558A FR2647119B1 (en) 1989-05-19 1989-05-19 PROCESS FOR CULTURING MICROORGANISMS ON A SOLID MEDIUM CONSISTING OF A SOLID, ABSORBENT, COMPRESSIBLE AND NON-FERMENTABLE SUPPORT

Publications (1)

Publication Number Publication Date
MX178723A true MX178723A (en) 1995-07-14

Family

ID=9381804

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2076790A MX178723A (en) 1989-05-19 1990-03-18 Microorganism culture procedure in a solid medium consisting of an understandable and non-fermentable solid absorbent support

Country Status (2)

Country Link
FR (1) FR2647119B1 (en)
MX (1) MX178723A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008130210A1 (en) 2007-04-19 2008-10-30 Biokab, S.A. De C. V. Process for producing vanilin from microorganisms immobilized by surface cuture

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AP302A (en) * 1991-07-01 1994-01-24 Agricura Private Ltd Production of trichoderma harzianum (T77)
MX2008012689A (en) 2007-04-19 2009-04-17 Biokab S A De C V Process for producing vanilin from microorganisms immobilized by surface cuture.
BE1031443A1 (en) * 2023-03-20 2024-10-14 Novobiom PROCESS FOR THE PRODUCTION OF BIOMOLECULES OF THERAPEUTIC OR PHARMACEUTICAL INTEREST BY SOLID FERMENTATION OF A FIBROUS SUBSTRATE

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62262987A (en) * 1986-05-09 1987-11-16 Rikagaku Kenkyusho Cell culture process

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008130210A1 (en) 2007-04-19 2008-10-30 Biokab, S.A. De C. V. Process for producing vanilin from microorganisms immobilized by surface cuture

Also Published As

Publication number Publication date
FR2647119A1 (en) 1990-11-23
FR2647119B1 (en) 1994-04-01

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