JPS5747479A - Production of enzyme - Google Patents
Production of enzymeInfo
- Publication number
- JPS5747479A JPS5747479A JP11986480A JP11986480A JPS5747479A JP S5747479 A JPS5747479 A JP S5747479A JP 11986480 A JP11986480 A JP 11986480A JP 11986480 A JP11986480 A JP 11986480A JP S5747479 A JPS5747479 A JP S5747479A
- Authority
- JP
- Japan
- Prior art keywords
- microorganism
- culture medium
- added
- cell wall
- inhibitor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: A inhibitor against cell wall formation is added to the culture medium to increase the capability of a microorganism in Becillus in producing enzymes.
CONSTITUTION: The culture medium contains a substance retarding assimilation such as starch as a carbon source, soybean protein and ammonium salt as nitrogen sources, metallic ions and other substances. As a microorganism, is used a strain in Bacillus, producing amylase, protease or ribonuclease and cultured aerobically by a usual method. As an inhibitor against cell wall formation, is used glycine, D- cycloserine, bacitracin, vancomycin or β-lactam antibiotic by 1W10-6%. The inhibitor is added, when the O.D. value reaches 0.5W1.0 and the cultivation is continued for additional one to three days, then the enzymes are collected.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11986480A JPS5747479A (en) | 1980-09-01 | 1980-09-01 | Production of enzyme |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11986480A JPS5747479A (en) | 1980-09-01 | 1980-09-01 | Production of enzyme |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5747479A true JPS5747479A (en) | 1982-03-18 |
Family
ID=14772153
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11986480A Pending JPS5747479A (en) | 1980-09-01 | 1980-09-01 | Production of enzyme |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5747479A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0061250A2 (en) * | 1981-03-23 | 1982-09-29 | Biogen N.V. | Improved process for recovering products produced by host organisms |
JPS58152485A (en) * | 1982-03-05 | 1983-09-10 | Ajinomoto Co Inc | Breeding method of microorganism |
EP0337009A2 (en) * | 1988-03-30 | 1989-10-18 | Kao Corporation | Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same |
-
1980
- 1980-09-01 JP JP11986480A patent/JPS5747479A/en active Pending
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0061250A2 (en) * | 1981-03-23 | 1982-09-29 | Biogen N.V. | Improved process for recovering products produced by host organisms |
EP0061250A3 (en) * | 1981-03-23 | 1983-09-28 | Biogen N.V. | Improved process for recovering products produced by host organisms |
JPS58152485A (en) * | 1982-03-05 | 1983-09-10 | Ajinomoto Co Inc | Breeding method of microorganism |
EP0337009A2 (en) * | 1988-03-30 | 1989-10-18 | Kao Corporation | Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same |
US5079154A (en) * | 1988-03-30 | 1992-01-07 | Kao Corporation | Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same |
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