JPS5747479A - Production of enzyme - Google Patents

Production of enzyme

Info

Publication number
JPS5747479A
JPS5747479A JP11986480A JP11986480A JPS5747479A JP S5747479 A JPS5747479 A JP S5747479A JP 11986480 A JP11986480 A JP 11986480A JP 11986480 A JP11986480 A JP 11986480A JP S5747479 A JPS5747479 A JP S5747479A
Authority
JP
Japan
Prior art keywords
microorganism
culture medium
added
cell wall
inhibitor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP11986480A
Other languages
Japanese (ja)
Inventor
Juzo Udaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP11986480A priority Critical patent/JPS5747479A/en
Publication of JPS5747479A publication Critical patent/JPS5747479A/en
Pending legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: A inhibitor against cell wall formation is added to the culture medium to increase the capability of a microorganism in Becillus in producing enzymes.
CONSTITUTION: The culture medium contains a substance retarding assimilation such as starch as a carbon source, soybean protein and ammonium salt as nitrogen sources, metallic ions and other substances. As a microorganism, is used a strain in Bacillus, producing amylase, protease or ribonuclease and cultured aerobically by a usual method. As an inhibitor against cell wall formation, is used glycine, D- cycloserine, bacitracin, vancomycin or β-lactam antibiotic by 1W10-6%. The inhibitor is added, when the O.D. value reaches 0.5W1.0 and the cultivation is continued for additional one to three days, then the enzymes are collected.
COPYRIGHT: (C)1982,JPO&Japio
JP11986480A 1980-09-01 1980-09-01 Production of enzyme Pending JPS5747479A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11986480A JPS5747479A (en) 1980-09-01 1980-09-01 Production of enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11986480A JPS5747479A (en) 1980-09-01 1980-09-01 Production of enzyme

Publications (1)

Publication Number Publication Date
JPS5747479A true JPS5747479A (en) 1982-03-18

Family

ID=14772153

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11986480A Pending JPS5747479A (en) 1980-09-01 1980-09-01 Production of enzyme

Country Status (1)

Country Link
JP (1) JPS5747479A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0061250A2 (en) * 1981-03-23 1982-09-29 Biogen N.V. Improved process for recovering products produced by host organisms
JPS58152485A (en) * 1982-03-05 1983-09-10 Ajinomoto Co Inc Breeding method of microorganism
EP0337009A2 (en) * 1988-03-30 1989-10-18 Kao Corporation Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0061250A2 (en) * 1981-03-23 1982-09-29 Biogen N.V. Improved process for recovering products produced by host organisms
EP0061250A3 (en) * 1981-03-23 1983-09-28 Biogen N.V. Improved process for recovering products produced by host organisms
JPS58152485A (en) * 1982-03-05 1983-09-10 Ajinomoto Co Inc Breeding method of microorganism
EP0337009A2 (en) * 1988-03-30 1989-10-18 Kao Corporation Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same
US5079154A (en) * 1988-03-30 1992-01-07 Kao Corporation Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same

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