MD850Z - Process for feeding of bee-families Apis mellifera - Google Patents

Process for feeding of bee-families Apis mellifera Download PDF

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MD850Z
MD850Z MDS20140060A MDS20140060A MD850Z MD 850 Z MD850 Z MD 850Z MD S20140060 A MDS20140060 A MD S20140060A MD S20140060 A MDS20140060 A MD S20140060A MD 850 Z MD850 Z MD 850Z
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feeding
bee
bees
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families
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Ион ТОДЕРАШ
Валентина ЧЕБОТАРЬ
Аурелиан ГУЛЯ
Ион БУЗУ
Валериу ТОДЕРИЧ
Ион БУЛЬМЕСТРУ
Олеся ГЛИГА
Надежда РАЙЛЯН
Андрей СИЛИТРАРЬ
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Институт Зоологии Академии Наук Молдовы
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Abstract

The invention relates to beekeeping, in particular to a process for feeding of bee-families Apis mellifera.The process, according to the invention, comprises feeding of bee-families with a mixture of 50% sugar syrup and an aqueous solution with the concentration of 1 mg% of [tris-cobalt (III)thiosemicarbazidate][1, 2-bismuth(III)diaminocyclohexanetetraacetate] hexahydrate sulfate - [Co(tios)3][Bi(cdta)]SO4·6H2O, taken in a ratio of 100:2 respectively, and feeding is carried out in the spring, in an amount of 100…130 ml per frame with bees, every 2 days, during 3 weeks.

Description

Invenţia se referă la apicultură, în special la un procedeu de hrănire a familiilor de albine Apis mellifera. The invention relates to beekeeping, in particular to a process for feeding Apis mellifera bee colonies.

În perioada de primăvară, după iernarea coloniei de albine, în organismul lor se constată o carenţă de substanţe nutritive bioactive, în special de microelemente, care au un rol catalizator în procesele fiziologice de activitate vitală a albinelor, îndeplinind funcţii multiple la nivel celular, intrând în componenţa unor enzime şi hormoni cu rol hotărâtor în metabolism. Insuficienţa substanţelor biologic active, în special a microelementelor, duce la slăbirea rezistenţei familiilor de albine la boli şi la diminuarea productivităţii lor. In the spring, after the bee colony has wintered, their bodies are deficient in bioactive nutrients, especially microelements, which have a catalytic role in the physiological processes of the bees' vital activity, performing multiple functions at the cellular level, being part of enzymes and hormones with a decisive role in metabolism. The insufficiency of biologically active substances, especially microelements, leads to a weakening of the bee colonies' resistance to diseases and a decrease in their productivity.

Principalele surse naturale de aprovizionare a organismului albinelor cu microelemente sunt nectarul şi polenul, culese de la florile plantelor melifere. Potrivit informaţiilor ştiinţifice, în miere şi polen se găsesc peste 30 de micro- macroelemente, inclusiv oligoelemente. Printre acestea, Co şi Bi se găsesc în cantităţi foarte mici, de la 10 la 14 µg%, dar rolul lor în metabolismul substanţelor este enorm. The main natural sources of microelements for bees are nectar and pollen, collected from the flowers of honey plants. According to scientific information, honey and pollen contain over 30 micro- and macroelements, including trace elements. Among them, Co and Bi are found in very small quantities, from 10 to 14 µg%, but their role in the metabolism of substances is enormous.

Dat fiind faptul că primăvara (perioadă deficitară de cules în natură), majoritatea apicultorilor hrănesc familiile de albine cu sirop de zahăr, în componenţa căruia mai mult de jumătate din microelementele necesare lipsesc, atunci identificarea surselor accesibile de oligoelemente pentru îmbogăţirea suplimentelor nutritive în raţia albinelor devine o problemă actuală. Given that in spring (a period of poor harvesting in nature), most beekeepers feed bee colonies with sugar syrup, which lacks more than half of the necessary microelements, identifying accessible sources of trace elements to enrich nutritional supplements in the bees' diet becomes a current problem.

Sunt cunoscute procedee de hrănire a familiilor de albine cu surse de microelemente, în special de cobalt sub formă de sare CoCl2 în doză de 8…25 mg la un litru de sirop de zahăr [1], dar eficienţa acestor procedee este foarte joasă, deoarece digerarea acestei sări şi asimilarea în organismul albinei a substanţelor bioactive sunt foarte slabe. There are known methods of feeding bee colonies with sources of microelements, especially cobalt in the form of CoCl2 salt in a dose of 8…25 mg per liter of sugar syrup [1], but the efficiency of these methods is very low, because the digestion of this salt and the assimilation of bioactive substances in the bee's body are very poor.

Cea mai apropiată soluţie, după esenţa tehnică şi rezultatul obţinut, este procedeul de hrănire a familiilor de albine Apis mellifera, ce include hrănirea albinelor primăvara cu un amestec de soluţie de 1% mas. de extract din biomasa tulpinii cianobacteriei Spirulina platensis CNM-CB-02 şi sirop de zahăr de 50%, luate în raport de 1:500, respectiv, tulpina cianobacteriei Spirulina platensis fiind cultivată în prezenţa compusului coordinativ selenitul de Fe(III) hexahidrat (FeSeO3·6H2O), iar hrănirea albinelor cu amestec se efectuează în cantitate de 100...130 ml la o ramă cu albine, la fiecare 2 zile, timp de două săptămâni [2]. The closest solution, according to the technical essence and the result obtained, is the process of feeding Apis mellifera bee colonies, which includes feeding the bees in the spring with a mixture of a 1% mass solution of extract from the biomass of the cyanobacterium strain Spirulina platensis CNM-CB-02 and 50% sugar syrup, taken in a ratio of 1:500, respectively, the strain of the cyanobacterium Spirulina platensis being cultivated in the presence of the coordinating compound Fe(III) selenite hexahydrate (FeSeO3·6H2O), and feeding the bees with the mixture is carried out in an amount of 100...130 ml per frame with bees, every 2 days, for two weeks [2].

Dezavantajul acestui procedeu constă în faptul că tehnologia obţinerii extractului biomasei tulpinii cianobacteriei Spirulina platensis, cultivate în prezenţa compusului coordinativ este prea complicată şi costisitoare, iar eficienţa utilizării microelementelor din compusul coordinativ administrat în cantităţi relativ mari, este scăzută. The disadvantage of this process is that the technology for obtaining the biomass extract of the cyanobacterial strain Spirulina platensis, cultivated in the presence of the coordinating compound, is too complicated and expensive, and the efficiency of using the microelements from the coordinating compound administered in relatively large quantities is low.

Problema pe care o rezolvă prezenta invenţie constă în elaborarea unui procedeu de hrănire a familiilor de albine în perioadele deficitare de cules în natură, care ar asigura necesităţile albinelor în Co şi Bi, ar spori rezistenţa la boli a familiilor de albine Apis mellifera, ar contribui la creşterea productivităţii lor. The problem solved by the present invention consists in developing a process for feeding bee colonies during periods of poor natural foraging, which would ensure the bees' needs in Co and Bi, increase the disease resistance of Apis mellifera bee colonies, and contribute to increasing their productivity.

Esenţa invenţiei constă în faptul că se propune un procedeu de hrănire a familiilor de albine Apis mellifera, care include hrănirea albinelor cu un amestec de sirop de zahăr de 50% şi o soluţie apoasă cu concentraţia de 1 mg% de sulfat de [tris-tiosemicarbazidat de cobalt(III)][1, 2-diaminociclohexantetraacetat de bismut(III)] hexahidrat - [Co(tios)3][Bi(cdta)]SO4· 6H2O (Compus+Co+Bi) luate în raport de 100:2 respectiv, iar hrănirea se efectuează primăvara, în cantitate de 100...130 ml la o ramă cu albine, la fiecare 2 zile, timp de 3 săptămâni. The essence of the invention consists in the fact that a process for feeding Apis mellifera bee colonies is proposed, which includes feeding the bees with a mixture of 50% sugar syrup and an aqueous solution with a concentration of 1 mg% of [cobalt(III) tris-thiosemicarbazidate][bismuth(III) 1, 2-diaminocyclohexanetetraacetate] sulfate hexahydrate - [Co(thios)3][Bi(cdta)]SO4· 6H2O (Compound+Co+Bi) taken in a ratio of 100:2 respectively, and the feeding is carried out in the spring, in an amount of 100...130 ml per frame with bees, every 2 days, for 3 weeks.

Pentru estimarea eficienţei procedeului de hrănire a albinelor cu suplimentul nutritiv sus-numit, în luna aprilie 2013, au fost desfăşurate experienţe de testare comparativă a acestuia pe familii de albine formate în trei loturi (vezi tabelul), câte 16 familii în fiecare lot, dintre care: lotul I - martor (albinele căruia au fost hrănite doar cu sirop de zahăr de 50%), lotul II - cea mai apropiată soluţie (albinele căruia primeau în hrană sirop de zahăr îmbogăţit cu suplimentul nutritiv Apispir+Fe+Se (MD 477 Z 2012.09.30)) şi lotul III - invenţia propusă (albinele căruia primeau ca hrană sirop de zahăr îmbogăţit cu suplimentul Compus+Co+Bi). To estimate the efficiency of the bee feeding process with the aforementioned nutritional supplement, in April 2013, comparative testing experiments were carried out on bee colonies formed into three batches (see table), 16 colonies in each batch, of which: batch I - control (the bees were fed only with 50% sugar syrup), batch II - the closest solution (the bees were fed sugar syrup enriched with the nutritional supplement Apispir+Fe+Se (MD 477 Z 2012.09.30)) and batch III - the proposed invention (the bees were fed sugar syrup enriched with the supplement Compus+Co+Bi).

Tabel Table

Valoarea caracterelor morfoproductive ale familiilor de albine The value of morphoproductive characters of bee colonies

din loturile experimentale from the experimental batches

Denumirea caracterelor Lotul I, martor (N* = 16) Lotul II, cea mai apropiată soluţie (N* = 16) Lotul III, invenţia propusă Compus+Co+Bi (N* = 16) Media caracterului % Media caracterului % faţă de lotul I Media caracterului % faţă de lotul I % faţă de lotul II Rezistenţa la boli, % 88,4 ± 0,4 100 90,5 ± 0,3 102,4 92,8 ± 0,3 105,0 102,5 Cantitatea de păstură, sute celule 90,5 ± 1,8 100 104,1 ± 1,5 115,0 111,6 ± 2,1 123,3 107,2 Cantitatea de miere, kg 11,62 ± 0,40 100 13,24 ± 0,40 113,9 14,57 ± 0,38 125,4 110,0 Cantitatea de ceară, kg 0,28 ± 0,01 100 0,34 ± 0,01 121,4 0,39 ± 0,01 139,3 114,7Character name Lot I, control (N* = 16) Lot II, closest solution (N* = 16) Lot III, proposed invention Compound+Co+Bi (N* = 16) Character average % Character average % compared to lot I Character average % compared to lot I % compared to lot II Disease resistance, % 88.4 ± 0.4 100 90.5 ± 0.3 102.4 92.8 ± 0.3 105.0 102.5 Pasture quantity, hundreds of cells 90.5 ± 1.8 100 104.1 ± 1.5 115.0 111.6 ± 2.1 123.3 107.2 Honey quantity, kg 11.62 ± 0.40 100 13.24 ± 0.40 113.9 14.57 ± 0.38 125.4 110.0 Wax quantity, kg 0.28 ± 0.01 100 0.34 ± 0.01 121.4 0.39 ± 0.01 139.3 114.7

Remarcă: N* - efectivul familiilor de albine în lot Note: N* - number of bee colonies in the batch

Datele obţinute în experienţe au fost prelucrate conform statisticii biometrice variaţionale (Плохинский Н.А. Руководство по биометрии для зоотехников. Москва, Колос, 1969, 256 p.). The data obtained in the experiments were processed according to variational biometric statistics (Plokhinsky N.A. Handbook of biometrics for zootechnicians. Moscow, Kolos, 1969, 256 p.).

Rezultatele testării au demonstrat că hrănirea albinelor cu suplimentul nutritiv Compus+Co+Bi, primăvara în perioada deficitară de cules, a contribuit la creşterea semnificativă a valorilor principalelor caractere de rezistenţă la boli (evaluate prin testul vitezei de igienă determinat conform Normei zootehnice privind bonitarea familiilor de albine, creşterea şi certificarea materialului genitor apicol, aprobată prin Hotărârea Guvernului Republicii Moldova nr. 306 din 2011.04.28 şi de acumulare a producţiilor apicole în cuib. The test results demonstrated that feeding bees with the nutritional supplement Compus+Co+Bi, in the spring during the deficient harvesting period, contributed to a significant increase in the values of the main disease resistance traits (evaluated by the hygiene speed test determined according to the Zootechnical Norm on the certification of bee colonies, the breeding and certification of beekeeping germinal material, approved by the Decision of the Government of the Republic of Moldova no. 306 of 2011.04.28 and the accumulation of beekeeping production in the nest.

S-a constatat că rezistenţa la boli a familiilor de albine din lotul III a crescut semnificativ, comparativ cu lotul I (martor) şi lotul II (cea mai apropiată soluţie), cu 4,4 şi 2,3 unităţi procentuale, sau cu 5,0 şi 2,5% (td = 8,8 şi 5,4; P < 0,001), respectiv. It was found that the disease resistance of bee colonies in group III increased significantly, compared to group I (control) and group II (closest solution), by 4.4 and 2.3 percentage units, or by 5.0 and 2.5% (td = 8.8 and 5.4; P < 0.001), respectively.

Substanţele biologic active din suplimentul Compus+Co+Bi au stimulat activitatea vitală a albinelor lucrătoare, contribuind la creşterea volumului de producţie acumulată în cuib. Astfel, familiile de albine din lotul III, la primul cules, au acumulat în cuib, comparativ, cu lotul I şi lotul II, o cantitate mai mare de păstură, cu 21,1 şi 7,5 sute celule sau cu 23,3 şi 7,2% (td = 7,6 şi 2,9; P < 0,001 şi P < 0,05), respectiv, o cantitate mai mare de miere, cu 2,95 şi 1,33 kg sau cu 25,4 şi 10,0% (td = 5,4 şi 2,4; P < 0,001 şi P < 0,05), respectiv, şi o cantitate mai mare de ceară, cu 0,11 şi 0,05 kg sau cu 39,3 şi 14,7% (td = 7,8 şi 3,6; P < 0,001), respectiv. The biologically active substances in the Compus+Co+Bi supplement stimulated the vital activity of worker bees, contributing to an increase in the volume of production accumulated in the nest. Thus, the bee colonies in batch III, at the first collection, accumulated in the nest, compared to batch I and batch II, a greater amount of brood, by 21.1 and 7.5 hundred cells or by 23.3 and 7.2% (td = 7.6 and 2.9; P < 0.001 and P < 0.05), respectively, a greater amount of honey, by 2.95 and 1.33 kg or by 25.4 and 10.0% (td = 5.4 and 2.4; P < 0.001 and P < 0.05), respectively, and a greater amount of wax, by 0.11 and 0.05 kg or by 39.3 and 14.7% (td = 7.8 and 3.6; P < 0.001), respectively.

Prin urmare, rezultatul tehnic al invenţiei constă în creşterea rezistenţei familiilor de albine la boli şi sporirea productivităţii lor de păstură, miere, ceară. Therefore, the technical result of the invention consists in increasing the resistance of bee colonies to diseases and increasing their productivity of pollen, honey, wax.

Rezultatul este determinat de substanţele biologic active din compusul organic coordinativ, în special de oligoelementele acestuia. Ionii de Co(III), fiind parte componentă a vitaminei B12, au un rol catalizator în metabolismul substanţelor azotate, cu influenţe directe asupra creşterii organismului, participă la sinteza unor enzime şi aminoacizi esenţiali (metionina, glicina, serina, colina), în sinteza acizilor nucleici, intervine în menţinerea integrităţii sistemului nervos central şi periferic, în secreţia lăptişorului de matcă. Toate acestea determină în mare măsură dezvoltarea larvelor şi activitatea vitală a albinelor lucrătoare, contribuind la creşterea productivităţii familiilor de albine în ansamblu. Se presupune că ionii de Bi (III), prin capacitatea lor de penetrabilitate a celulelor vii, activizează procesul de proliferare a celulelor devoratoare de bacterii şi de sinteză în hemolimfă a unor substanţe apidecine cu acţiune antibacteriană, care fortifică sistemul imunitar al albinelor şi sporesc rezistenţa coloniilor acestora la boli. The result is determined by the biologically active substances in the coordinating organic compound, especially its trace elements. Co(III) ions, being a component of vitamin B12, have a catalytic role in the metabolism of nitrogenous substances, with direct influences on the growth of the organism, participate in the synthesis of some enzymes and essential amino acids (methionine, glycine, serine, choline), in the synthesis of nucleic acids, intervene in maintaining the integrity of the central and peripheral nervous system, in the secretion of royal jelly. All of this largely determines the development of larvae and the vital activity of worker bees, contributing to increasing the productivity of bee colonies as a whole. It is assumed that Bi(III) ions, through their ability to penetrate living cells, activate the process of proliferation of bacteria-devouring cells and synthesis in the hemolymph of some apidecines with antibacterial action, which strengthen the immune system of bees and increase the resistance of their colonies to diseases.

Exemplu de realizare a invenţiei Example of embodiment of the invention

Procedeul de hrănire a familiilor de albine Apis mellifera se realizează în felul următor. The process of feeding Apis mellifera bee colonies is carried out as follows.

Pentru obţinerea suplimentului bioactiv Compus+Co+Bi se dizolvă 10 mg de substanţă uscată a compusului organic coordinativ heteronuclear sulfatul de [tris-tiosemicarbazidat de cobalt(III)][1, 2-diaminociclohexantetraacetat de bismut(III)] hexahidrat - [Co(tios)3][Bi(cdta)]SO4· 6H2O în 1000 ml de apă distilată. Soluţia apoasă de supliment se păstrează la răcoare (t° = +4 ... +16°C), în loc întunecat. Ulterior, se amestecă suplimentul bioactiv cu siropul de zahăr de 50% încălzit la temperatura de 30 ... 35°C, în raport de 2:100, respectiv. Amestecul nutritiv se distribuie albinelor în hrănitorul stupului primăvara, în perioada deficitară de cules în natură, în cantitate de 100 ml pentru fiecare interval de rame populat cu albine, la fiecare 2 zile, timp de trei săptămâni. To obtain the bioactive supplement Compus+Co+Bi, dissolve 10 mg of dry matter of the heteronuclear coordinating organic compound [tris-thiosemicarbazidate of cobalt(III)][1, 2-diaminocyclohexanetetraacetate of bismuth(III)] sulfate hexahydrate - [Co(thios)3][Bi(cdta)]SO4· 6H2O in 1000 ml of distilled water. The aqueous solution of the supplement is kept cool (t° = +4 ... +16°C), in a dark place. Subsequently, mix the bioactive supplement with 50% sugar syrup heated to a temperature of 30 ... 35°C, in a ratio of 2:100, respectively. The nutritional mixture is distributed to the bees in the hive feeder in the spring, during the scarce period of natural collection, in an amount of 100 ml for each frame interval populated with bees, every 2 days, for three weeks.

1. Яковлев Ф.С. Итоги исследований по выявлению стимулирующих подкормок на семьи пчёл. Труды НИИ пчеловодства, Москва, Московский рабочий, 1972, вып. 7, p. 87-101 1. Yakovlev F.S. Results of studies on the identification of stimulating supplements for bee families. Труды НИИ пчеловодства, Москва, Московский рабочий, 1972, вып. 7, p. 87-101

2. MD 477 Y 2012.02.29 2. MD 477 Y 2012.02.29

Claims (1)

Procedeu de hrănire a familiilor de albine Apis mellifera, care include hrănirea albinelor cu un amestec de sirop de zahăr de 50% şi o soluţie apoasă cu concentraţia de 1 mg% de sulfat de [tris-tiosemicarbazidat de cobalt(III)][1, 2-diaminociclohexantetraacetat de bismut(III)] hexahidrat - [Co(tios)3][Bi(cdta)]SO4· 6H2O, luate în raport de 100:2 respectiv, iar hrănirea se efectuează primăvara, în cantitate de 100...130 ml la o ramă cu albine, la fiecare 2 zile, timp de 3 săptămâni.Method of feeding Apis mellifera bee colonies, which includes feeding the bees with a mixture of 50% sugar syrup and an aqueous solution with a concentration of 1 mg% of [cobalt(III) tris-thiosemicarbazidate][bismuth(III) 1, 2-diaminocyclohexanetetraacetate] sulfate hexahydrate - [Co(thios)3][Bi(cdta)]SO4· 6H2O, taken in a ratio of 100:2 respectively, and the feeding is carried out in the spring, in an amount of 100...130 ml per frame with bees, every 2 days, for 3 weeks.
MDS20140060A 2014-05-07 2014-05-07 Process for feeding of bee-families Apis mellifera MD850Z (en)

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MD4438C1 (en) * 2014-08-22 2017-05-31 Институт Зоологии Академии Наук Молдовы Process for the production of [tetraoxoethylenediamine tetraacetatedimolibdenum V] bis-(tetraphenylphosphonium)di-hemihydrate compound and process for feeding bees with its use

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MD20Z (en) * 2008-12-18 2010-01-31 Институт Зоологии Академии Наук Молдовы Method for prophylaxis and treatment of American foulbrood of bees
MD477Y (en) * 2011-06-01 2012-02-29 Inst De Zool Al Akademiei De Shtiintse A Republichii Moldova Process for feeding bee families Apis mellifera
MD476Z (en) * 2011-06-01 2012-09-30 Институт Зоологии Академии Наук Молдовы Process for feeding bee families Apis mellifera
MD475Z (en) * 2011-06-01 2012-09-30 Институт Зоологии Академии Наук Молдовы Process for feeding bee families Apis mellifera
MD538Z (en) * 2012-01-30 2013-03-31 Николае ЕРЕМИЯ Process for raising bee colonies

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