MD4038C1 - Method of predicting the risk of development of the thyroid gland cancer - Google Patents

Method of predicting the risk of development of the thyroid gland cancer

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Publication number
MD4038C1
MD4038C1 MDA20100035A MD20100035A MD4038C1 MD 4038 C1 MD4038 C1 MD 4038C1 MD A20100035 A MDA20100035 A MD A20100035A MD 20100035 A MD20100035 A MD 20100035A MD 4038 C1 MD4038 C1 MD 4038C1
Authority
MD
Moldova
Prior art keywords
dna
thyroid gland
risk
development
gland cancer
Prior art date
Application number
MDA20100035A
Other languages
Romanian (ro)
Russian (ru)
Other versions
MD4038B1 (en
Inventor
Виктор Попеску
Original Assignee
Univ Nicolae Testemitanu
Univ De Stat De Medicin & Abreve & Scedil I Farmacie Nicolae Testemi & Tcedil Anu Din Republica Mold
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nicolae Testemitanu, Univ De Stat De Medicin & Abreve & Scedil I Farmacie Nicolae Testemi & Tcedil Anu Din Republica Mold filed Critical Univ Nicolae Testemitanu
Priority to MDA20100035A priority Critical patent/MD4038C1/en
Publication of MD4038B1 publication Critical patent/MD4038B1/en
Publication of MD4038C1 publication Critical patent/MD4038C1/en

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Abstract

The invention relates to medicine, particularly preventive medicine and can be used to predict the risk of development of the thyroid gland cancer.According to the invention, the method of predicting the risk of development of the thyroid gland cancer consists in that it is carried out intake of a tissue fragment from the tumor of thyroid gland, it is isolated therefrom the genomic DNA by enzymatic digestion with K proteinase, it is modified the DNA through the conversion of cytosine to uracil by the method Qiagen EpiTect Bisulfite Kit, then the modified DNA is copied using primers corresponding to DNA of methylated and unmethylated gene p15 by the method Methylation Specific Polimerase Chain Reaction, the products are introduced in the gel for electrophoresis of agarose diluted in tris-borate-ethylenediaminetetraacetate and dyed with ethidium bromide, the gel is irradiated with ultra-violet radiation with a wavelength of 312 nm and where it is visible only the band of methylated DNA, it is predicted the risk of development of the thyroid gland cancer, and where is revealed the band of methylated DNA and the band of unmethylated DNA - lack of the risk of development of the thyroid gland cancer.
MDA20100035A 2010-03-03 2010-03-03 Method of predicting the risk of development of the thyroid gland cancer MD4038C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MDA20100035A MD4038C1 (en) 2010-03-03 2010-03-03 Method of predicting the risk of development of the thyroid gland cancer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MDA20100035A MD4038C1 (en) 2010-03-03 2010-03-03 Method of predicting the risk of development of the thyroid gland cancer

Publications (2)

Publication Number Publication Date
MD4038B1 MD4038B1 (en) 2010-05-31
MD4038C1 true MD4038C1 (en) 2011-01-31

Family

ID=43568954

Family Applications (1)

Application Number Title Priority Date Filing Date
MDA20100035A MD4038C1 (en) 2010-03-03 2010-03-03 Method of predicting the risk of development of the thyroid gland cancer

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MD (1) MD4038C1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014085434A1 (en) 2012-11-27 2014-06-05 Pontificia Universidad Catolica De Chile Compositions and methods for diagnosing thyroid tumors

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU619859A1 (en) * 1976-12-06 1978-08-15 Научно-исследовательский институт медицинской радиологии Method of differential diagnostics of thyroid gland cancer
SU1063390A1 (en) * 1982-01-07 1983-12-30 Всесоюзный онкологический научный центр АМН СССР Method of diagnosis of thyroid gland cancer
SU1807409A1 (en) * 1991-01-28 1993-04-07 Kh Nii Med Radiologii Method for diagnosis of thyroid gland cancer
RU2190346C2 (en) * 1999-04-20 2002-10-10 Шилко Мария Владимировна Method for evaluating functional state of thyroid gland
RU2229262C1 (en) * 2003-03-19 2004-05-27 Вараксин Виктор Васильевич Method for predicting malignant thyroid tumors
RU2247376C1 (en) * 2003-06-25 2005-02-27 Савина Лидия Васильевна Rapid diagnosis method for diagnosing structural changes in human thyroid gland hyperfunction
RU2253360C2 (en) * 2003-04-17 2005-06-10 Шигаев Михаил Юрьевич Method for carrying out biological tissue edema monitoring
UA22831U (en) * 2006-12-26 2007-04-25 Oleksandr Pavlovych Nechai Method of preoperative diagnosticating for detecting medullary cancer of the thyroid gland
RU2300106C1 (en) * 2005-10-17 2007-05-27 Ростовский научно-исследовательский онкологический институт Федерального агентства по здравоохранению и социальному развитию Method for setting differential diagnosis of thyroid gland malignant tumors in children and adolescent
RU2316260C2 (en) * 2005-11-01 2008-02-10 ГУ Дальневосточный научный центр физиологии и патологии дыхания Сибирского отделения Российской академии медицинских наук Method for evaluating the risk in the development of iodine-deficient state in population of administrative region under conditions of anthropotechnogenic chemical environmental pollution

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU619859A1 (en) * 1976-12-06 1978-08-15 Научно-исследовательский институт медицинской радиологии Method of differential diagnostics of thyroid gland cancer
SU1063390A1 (en) * 1982-01-07 1983-12-30 Всесоюзный онкологический научный центр АМН СССР Method of diagnosis of thyroid gland cancer
SU1807409A1 (en) * 1991-01-28 1993-04-07 Kh Nii Med Radiologii Method for diagnosis of thyroid gland cancer
RU2190346C2 (en) * 1999-04-20 2002-10-10 Шилко Мария Владимировна Method for evaluating functional state of thyroid gland
RU2229262C1 (en) * 2003-03-19 2004-05-27 Вараксин Виктор Васильевич Method for predicting malignant thyroid tumors
RU2253360C2 (en) * 2003-04-17 2005-06-10 Шигаев Михаил Юрьевич Method for carrying out biological tissue edema monitoring
RU2247376C1 (en) * 2003-06-25 2005-02-27 Савина Лидия Васильевна Rapid diagnosis method for diagnosing structural changes in human thyroid gland hyperfunction
RU2300106C1 (en) * 2005-10-17 2007-05-27 Ростовский научно-исследовательский онкологический институт Федерального агентства по здравоохранению и социальному развитию Method for setting differential diagnosis of thyroid gland malignant tumors in children and adolescent
RU2316260C2 (en) * 2005-11-01 2008-02-10 ГУ Дальневосточный научный центр физиологии и патологии дыхания Сибирского отделения Российской академии медицинских наук Method for evaluating the risk in the development of iodine-deficient state in population of administrative region under conditions of anthropotechnogenic chemical environmental pollution
UA22831U (en) * 2006-12-26 2007-04-25 Oleksandr Pavlovych Nechai Method of preoperative diagnosticating for detecting medullary cancer of the thyroid gland

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Hoque M. O., Rosenbaum E. et al. Quantitative assesment of promoter methylation profiles in thyroid neoplasms. The Journal of Clinical Endocrinology & Metabolism, 90 (7), 2005, p. 4011-4018 *

Also Published As

Publication number Publication date
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