MD4038C1 - Procedeu de prognozare a riscului de dezvoltare a cancerului glandei tiroide - Google Patents
Procedeu de prognozare a riscului de dezvoltare a cancerului glandei tiroideInfo
- Publication number
- MD4038C1 MD4038C1 MDA20100035A MD20100035A MD4038C1 MD 4038 C1 MD4038 C1 MD 4038C1 MD A20100035 A MDA20100035 A MD A20100035A MD 20100035 A MD20100035 A MD 20100035A MD 4038 C1 MD4038 C1 MD 4038C1
- Authority
- MD
- Moldova
- Prior art keywords
- dna
- thyroid gland
- risk
- development
- gland cancer
- Prior art date
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Invenţia se referă la medicină, în special la medicina preventivă şi poate fi utilizată la prognozarea riscului dezvoltării cancerului glandei tiroide.Conform invenţiei, procedeul de prognozare a riscului de dezvoltare a cancerului glandei tiroide constă în aceea că se prelevează un fragment de ţesut din tumoarea glandei tiroide, se extrage din acesta ADN-ul genomic prin digerare enzimatică cu proteinaza K, se modifică ADN-ul prin conversia citozinei în uracil prin metoda Qiagen EpiTect Bisulfite Kit, apoi ADN-ul modificat se amplifică cu ajutorul unor praimeri corespunzători ADN-ului genei p15 metilate şi celei nemetilate prin metoda Methylation Specific Polimerase Chain Reaction, produşii se introduc într-un gel de electroforeză din agaroză dizolvată în tris-borat-etilendiamintetraacetat şi se colorează cu bromură de etidiu, gelul se iradiază cu radiaţie ultravioletă cu lungimea de undă de 312 nm, şi în cazul, în care se vizualizează numai banda AND-ului metilat, se prognozează riscul de dezvoltare a cancerului glandei tiroide, iar în cazul în care se vizualizează banda AND-ului metilat şi banda AND-ului nemetilat - lipsa riscului de dezvoltare a cancerului glandei tiroide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20100035A MD4038C1 (ro) | 2010-03-03 | 2010-03-03 | Procedeu de prognozare a riscului de dezvoltare a cancerului glandei tiroide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20100035A MD4038C1 (ro) | 2010-03-03 | 2010-03-03 | Procedeu de prognozare a riscului de dezvoltare a cancerului glandei tiroide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD4038B1 MD4038B1 (ro) | 2010-05-31 |
| MD4038C1 true MD4038C1 (ro) | 2011-01-31 |
Family
ID=43568954
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20100035A MD4038C1 (ro) | 2010-03-03 | 2010-03-03 | Procedeu de prognozare a riscului de dezvoltare a cancerului glandei tiroide |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD4038C1 (ro) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014085434A1 (en) | 2012-11-27 | 2014-06-05 | Pontificia Universidad Catolica De Chile | Compositions and methods for diagnosing thyroid tumors |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU619859A1 (ru) * | 1976-12-06 | 1978-08-15 | Научно-исследовательский институт медицинской радиологии | Способ дифференциальной диагностики рака щитовидной железы |
| SU1063390A1 (ru) * | 1982-01-07 | 1983-12-30 | Всесоюзный онкологический научный центр АМН СССР | Способ диагностики рака щитовидной железы |
| SU1807409A1 (ru) * | 1991-01-28 | 1993-04-07 | Kh Nii Med Radiologii | Способ диагностики рака щитовидной железы |
| RU2190346C2 (ru) * | 1999-04-20 | 2002-10-10 | Шилко Мария Владимировна | Способ оценки функционального состояния щитовидной железы |
| RU2229262C1 (ru) * | 2003-03-19 | 2004-05-27 | Вараксин Виктор Васильевич | Способ диагностики злокачественных опухолей щитовидной железы |
| RU2247376C1 (ru) * | 2003-06-25 | 2005-02-27 | Савина Лидия Васильевна | Способ экспресс-диагностики структурных изменений в щитовидной железе человека |
| RU2253360C2 (ru) * | 2003-04-17 | 2005-06-10 | Шигаев Михаил Юрьевич | Способ мониторинга отека биологической ткани |
| UA22831U (en) * | 2006-12-26 | 2007-04-25 | Oleksandr Pavlovych Nechai | Method of preoperative diagnosticating for detecting medullary cancer of the thyroid gland |
| RU2300106C1 (ru) * | 2005-10-17 | 2007-05-27 | Ростовский научно-исследовательский онкологический институт Федерального агентства по здравоохранению и социальному развитию | Способ дифференциальной диагностики злокачественных опухолей щитовидной железы у детей и подростков |
| RU2316260C2 (ru) * | 2005-11-01 | 2008-02-10 | ГУ Дальневосточный научный центр физиологии и патологии дыхания Сибирского отделения Российской академии медицинских наук | Способ оценки риска развития йоддефицитного состояния у населения административной территории в условиях антропотехногенного химического загрязнения окружающей среды |
-
2010
- 2010-03-03 MD MDA20100035A patent/MD4038C1/ro not_active IP Right Cessation
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU619859A1 (ru) * | 1976-12-06 | 1978-08-15 | Научно-исследовательский институт медицинской радиологии | Способ дифференциальной диагностики рака щитовидной железы |
| SU1063390A1 (ru) * | 1982-01-07 | 1983-12-30 | Всесоюзный онкологический научный центр АМН СССР | Способ диагностики рака щитовидной железы |
| SU1807409A1 (ru) * | 1991-01-28 | 1993-04-07 | Kh Nii Med Radiologii | Способ диагностики рака щитовидной железы |
| RU2190346C2 (ru) * | 1999-04-20 | 2002-10-10 | Шилко Мария Владимировна | Способ оценки функционального состояния щитовидной железы |
| RU2229262C1 (ru) * | 2003-03-19 | 2004-05-27 | Вараксин Виктор Васильевич | Способ диагностики злокачественных опухолей щитовидной железы |
| RU2253360C2 (ru) * | 2003-04-17 | 2005-06-10 | Шигаев Михаил Юрьевич | Способ мониторинга отека биологической ткани |
| RU2247376C1 (ru) * | 2003-06-25 | 2005-02-27 | Савина Лидия Васильевна | Способ экспресс-диагностики структурных изменений в щитовидной железе человека |
| RU2300106C1 (ru) * | 2005-10-17 | 2007-05-27 | Ростовский научно-исследовательский онкологический институт Федерального агентства по здравоохранению и социальному развитию | Способ дифференциальной диагностики злокачественных опухолей щитовидной железы у детей и подростков |
| RU2316260C2 (ru) * | 2005-11-01 | 2008-02-10 | ГУ Дальневосточный научный центр физиологии и патологии дыхания Сибирского отделения Российской академии медицинских наук | Способ оценки риска развития йоддефицитного состояния у населения административной территории в условиях антропотехногенного химического загрязнения окружающей среды |
| UA22831U (en) * | 2006-12-26 | 2007-04-25 | Oleksandr Pavlovych Nechai | Method of preoperative diagnosticating for detecting medullary cancer of the thyroid gland |
Non-Patent Citations (1)
| Title |
|---|
| Hoque M. O., Rosenbaum E. et al. Quantitative assesment of promoter methylation profiles in thyroid neoplasms. The Journal of Clinical Endocrinology & Metabolism, 90 (7), 2005, p. 4011-4018 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD4038B1 (ro) | 2010-05-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |