MD4038B1 - Method of predicting the risk of development of the thyroid gland cancer - Google Patents
Method of predicting the risk of development of the thyroid gland cancerInfo
- Publication number
- MD4038B1 MD4038B1 MDA20100035A MD20100035A MD4038B1 MD 4038 B1 MD4038 B1 MD 4038B1 MD A20100035 A MDA20100035 A MD A20100035A MD 20100035 A MD20100035 A MD 20100035A MD 4038 B1 MD4038 B1 MD 4038B1
- Authority
- MD
- Moldova
- Prior art keywords
- risk
- thyroid gland
- dna
- methylated
- gland cancer
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Inventia se refera la medicina, in special la medicina preventiva si poate fi utilizata la prognozarea riscului dezvoltarii cancerului glandei tiroide. Conform inventiei, procedeul de prognozare a riscului de dezvoltare a cancerului glandei tiroide consta in aceea ca se preleveaza un fragment de tesut din tumoarea glandei tiroide, se extrage din acesta ADN-ul genomic prin digerare enzimatica cu proteinaza K, se modifica ADN-ul prin conversia citozinei in uracil prin metoda Qiagen EpiTect Bisulfite Kit, apoi ADN-ul modificat se amplifica cu ajutorul unor praimeri corespunzatori ADN-ului genei p15 metilate si celei nemetilate prin metoda Methylation Specific Polimerase Chain Reaction, produsii se introduc intr-un gel de electroforeza din agaroza dizolvata in tris-borat-etilendiamintetraacetat si se coloreaza cu bromura de etidiu, gelul se iradiaza cu radiatie ultravioleta cu lungimea de unda de 312 nm, si in cazul, in care se vizualizeaza numai banda AND-ului metilat, se prognozeaza riscul de dezvoltare a cancerului glandei tiroide, iar in cazul in care se vizualizeaza banda AND-ului metilat si banda AND-ului nemetilat - lipsa riscului de dezvoltare a cancerului glandei tiroide.The invention relates to medicine, in particular to preventive medicine and can be used to predict the risk of developing thyroid gland cancer. According to the invention, the process for predicting the risk of developing thyroid gland cancer consists in the fact that a tissue fragment is taken from the tumor of the thyroid gland, the genomic DNA is extracted from it by enzymatic digestion with proteinase K, the DNA is modified by the conversion of cytosine to uracil by the Qiagen EpiTect Bisulfite Kit method, then the modified DNA is amplified by DNA-matching progenitors of the p15 methylated and unmethylated DNA by the Methylation Specific Polymerase Chain Reaction method, the products are introduced into an electrophoresis gel of agarose dissolved in tris-borate-ethylenediaminetetraacetate and stained with ethidium bromide, the gel is irradiated with ultraviolet radiation with a wavelength of 312 nm, and if only the methylated AND band is visualized, the risk of development is predicted of thyroid gland cancer, and if the methylated AND and non-methylated AND band are visualized - lack of risk thyroid cancer development.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDA20100035A MD4038C1 (en) | 2010-03-03 | 2010-03-03 | Method of predicting the risk of development of the thyroid gland cancer |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDA20100035A MD4038C1 (en) | 2010-03-03 | 2010-03-03 | Method of predicting the risk of development of the thyroid gland cancer |
Publications (2)
Publication Number | Publication Date |
---|---|
MD4038B1 true MD4038B1 (en) | 2010-05-31 |
MD4038C1 MD4038C1 (en) | 2011-01-31 |
Family
ID=43568954
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MDA20100035A MD4038C1 (en) | 2010-03-03 | 2010-03-03 | Method of predicting the risk of development of the thyroid gland cancer |
Country Status (1)
Country | Link |
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MD (1) | MD4038C1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10260103B2 (en) | 2012-11-27 | 2019-04-16 | Pontificia Universidad Catolica De Chile | Compositions and methods for diagnosing thyroid tumors |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU619859A1 (en) * | 1976-12-06 | 1978-08-15 | Научно-исследовательский институт медицинской радиологии | Method of differential diagnostics of thyroid gland cancer |
SU1063390A1 (en) * | 1982-01-07 | 1983-12-30 | Всесоюзный онкологический научный центр АМН СССР | Method of diagnosis of thyroid gland cancer |
SU1807409A1 (en) * | 1991-01-28 | 1993-04-07 | Kh Nii Med Radiologii | Method for diagnosis of thyroid gland cancer |
RU2190346C2 (en) * | 1999-04-20 | 2002-10-10 | Шилко Мария Владимировна | Method for evaluating functional state of thyroid gland |
RU2229262C1 (en) * | 2003-03-19 | 2004-05-27 | Вараксин Виктор Васильевич | Method for predicting malignant thyroid tumors |
RU2253360C2 (en) * | 2003-04-17 | 2005-06-10 | Шигаев Михаил Юрьевич | Method for carrying out biological tissue edema monitoring |
RU2247376C1 (en) * | 2003-06-25 | 2005-02-27 | Савина Лидия Васильевна | Rapid diagnosis method for diagnosing structural changes in human thyroid gland hyperfunction |
RU2300106C1 (en) * | 2005-10-17 | 2007-05-27 | Ростовский научно-исследовательский онкологический институт Федерального агентства по здравоохранению и социальному развитию | Method for setting differential diagnosis of thyroid gland malignant tumors in children and adolescent |
RU2316260C2 (en) * | 2005-11-01 | 2008-02-10 | ГУ Дальневосточный научный центр физиологии и патологии дыхания Сибирского отделения Российской академии медицинских наук | Method for evaluating the risk in the development of iodine-deficient state in population of administrative region under conditions of anthropotechnogenic chemical environmental pollution |
UA22831U (en) * | 2006-12-26 | 2007-04-25 | Oleksandr Pavlovych Nechai | Method of preoperative diagnosticating for detecting medullary cancer of the thyroid gland |
-
2010
- 2010-03-03 MD MDA20100035A patent/MD4038C1/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10260103B2 (en) | 2012-11-27 | 2019-04-16 | Pontificia Universidad Catolica De Chile | Compositions and methods for diagnosing thyroid tumors |
Also Published As
Publication number | Publication date |
---|---|
MD4038C1 (en) | 2011-01-31 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG4A | Patent for invention issued | ||
KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
MM4A | Patent for invention definitely lapsed due to non-payment of fees |