MD4030C2 - Base for the preparation of nutrient media for cultivation of microorganisms of Mycobacterium genus and process for the obtaining thereof - Google Patents
Base for the preparation of nutrient media for cultivation of microorganisms of Mycobacterium genus and process for the obtaining thereof Download PDFInfo
- Publication number
- MD4030C2 MD4030C2 MDA20090134A MD20090134A MD4030C2 MD 4030 C2 MD4030 C2 MD 4030C2 MD A20090134 A MDA20090134 A MD A20090134A MD 20090134 A MD20090134 A MD 20090134A MD 4030 C2 MD4030 C2 MD 4030C2
- Authority
- MD
- Moldova
- Prior art keywords
- microorganisms
- base
- cultivation
- obtaining
- preparation
- Prior art date
Links
- 241000186359 Mycobacterium Species 0.000 title abstract 3
- 244000005700 microbiome Species 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 235000015097 nutrients Nutrition 0.000 title abstract 2
- 238000002360 preparation method Methods 0.000 title abstract 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 4
- 238000004090 dissolution Methods 0.000 abstract 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract 2
- UMEAURNTRYCPNR-UHFFFAOYSA-N azane;iron(2+) Chemical compound N.[Fe+2] UMEAURNTRYCPNR-UHFFFAOYSA-N 0.000 abstract 2
- 239000012153 distilled water Substances 0.000 abstract 2
- 235000013922 glutamic acid Nutrition 0.000 abstract 2
- 239000004220 glutamic acid Substances 0.000 abstract 2
- 235000011187 glycerol Nutrition 0.000 abstract 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 2
- 235000019341 magnesium sulphate Nutrition 0.000 abstract 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 2
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 abstract 2
- 239000001509 sodium citrate Substances 0.000 abstract 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 abstract 2
- 235000011083 sodium citrates Nutrition 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to medicine, namely to the clinical microbiology and may be used for the diagnosis of microorganisms of Mycobacterium genus.It is proposed a base for the preparation of nutrient media for cultivation of microorganisms of Mycobacterium genus, containing: magnesium sulphate, sodium citrate, potassium dihydrogen phosphate, glutamic acid, 20% sodium hydroxide, ammonium iron alums, glycerine and distilled water in the following ratio of ingredients, in mass %:It is also proposed a process for the obtaining of the said base, including dissolution in distilled water of magnesium sulphate, sodium citrate and potassium dihydrogen phosphate, heating of the solution up to the temperature of 90°C, dissolution therein of glutamic acid and addition of 20% sodium hydroxide, cooling and dissolution in the obtained solution of ammonium iron alums and glycerine with further sterilization at a pressure of 0.5 atm., during 15 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20090134A MD4030C2 (en) | 2009-12-12 | 2009-12-12 | Base for the preparation of nutrient media for cultivation of microorganisms of Mycobacterium genus and process for the obtaining thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20090134A MD4030C2 (en) | 2009-12-12 | 2009-12-12 | Base for the preparation of nutrient media for cultivation of microorganisms of Mycobacterium genus and process for the obtaining thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD4030B1 MD4030B1 (en) | 2010-04-30 |
| MD4030C2 true MD4030C2 (en) | 2010-11-30 |
Family
ID=43569589
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20090134A MD4030C2 (en) | 2009-12-12 | 2009-12-12 | Base for the preparation of nutrient media for cultivation of microorganisms of Mycobacterium genus and process for the obtaining thereof |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD4030C2 (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU325879A1 (en) * | 1970-05-11 | 1973-02-08 | Э. Р. Финн | LIOTEKD I |
| SU1555358A1 (en) * | 1988-06-13 | 1990-04-07 | Молдавский Научно-Исследовательский Институт Туберкулеза | Nutrient medium for growing tuberculosis microbacteria |
| RU2059715C1 (en) * | 1993-06-15 | 1996-05-10 | Научно-исследовательский ветеринарный институт Нечерноземной зоны РФ | Salt base of nutrient medium for tuberculosis mycobacterium cultivation |
| MD727F2 (en) * | 1995-02-23 | 1997-05-31 | Valeriu Crudu | Medium for cultivation of the tuberculosis mycobacteria |
| MD812G2 (en) * | 1996-08-22 | 1998-05-31 | Crudu Valeriu | Agar medium for tuberculosis micobacteria cultivation |
| RU2121000C1 (en) * | 1995-07-11 | 1998-10-27 | Прикаспийский зональный научно-исследовательский ветеринарный институт | Nutrient medium for mycobacterium culturing |
| RU2192471C2 (en) * | 1995-06-02 | 2002-11-10 | Гист-Брокейдс Б.В. | METHOD OF 7-aminodesacetoxycephalospore acid PREPARING BY EFFECT OF EXPANDASE ACTIVITY OF PENICILLIN G |
| RU2193061C1 (en) * | 2001-05-04 | 2002-11-20 | Научно-производственное объединение "Питательные среды" | Nutrient medium for tuberculosis mycobacterium culturing (mbt-broth) |
| RU2215039C2 (en) * | 2001-07-31 | 2003-10-27 | Прикаспийский зональный научно-исследовательский ветеринарный институт | Nutrient medium for mycobacterium isolation and growing |
| RU2295561C2 (en) * | 2005-02-15 | 2007-03-20 | Государственное учреждение Якутский научно-исследовательский институт туберкулеза Министерства здравоохранения Республики Саха (Якутия) (ГУ Якутский НИИ туберкулеза МЗ РС (Я)) | Method for production of medium for mycobacterium tuberculosis (mbt) culturing |
| RU2320716C2 (en) * | 2006-04-17 | 2008-03-27 | Государственное научное учреждение Научно-исследовательский ветеринарный институт Нечерноземной зоны РФ Российской академии сельскохозяйственных наук | Solid nutrient medium for mycobacterium culturing |
| RU2322495C2 (en) * | 2006-04-17 | 2008-04-20 | Государственное научное учреждение Научно-исследовательский ветеринарный институт Нечерноземной зоны РФ Российской академии сельскохозяйственных наук | Nutrient medium for culturing mycobacterium and nocardioformous actinomyces |
-
2009
- 2009-12-12 MD MDA20090134A patent/MD4030C2/en not_active IP Right Cessation
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU325879A1 (en) * | 1970-05-11 | 1973-02-08 | Э. Р. Финн | LIOTEKD I |
| SU1555358A1 (en) * | 1988-06-13 | 1990-04-07 | Молдавский Научно-Исследовательский Институт Туберкулеза | Nutrient medium for growing tuberculosis microbacteria |
| RU2059715C1 (en) * | 1993-06-15 | 1996-05-10 | Научно-исследовательский ветеринарный институт Нечерноземной зоны РФ | Salt base of nutrient medium for tuberculosis mycobacterium cultivation |
| MD727F2 (en) * | 1995-02-23 | 1997-05-31 | Valeriu Crudu | Medium for cultivation of the tuberculosis mycobacteria |
| RU2192471C2 (en) * | 1995-06-02 | 2002-11-10 | Гист-Брокейдс Б.В. | METHOD OF 7-aminodesacetoxycephalospore acid PREPARING BY EFFECT OF EXPANDASE ACTIVITY OF PENICILLIN G |
| RU2121000C1 (en) * | 1995-07-11 | 1998-10-27 | Прикаспийский зональный научно-исследовательский ветеринарный институт | Nutrient medium for mycobacterium culturing |
| MD812G2 (en) * | 1996-08-22 | 1998-05-31 | Crudu Valeriu | Agar medium for tuberculosis micobacteria cultivation |
| RU2193061C1 (en) * | 2001-05-04 | 2002-11-20 | Научно-производственное объединение "Питательные среды" | Nutrient medium for tuberculosis mycobacterium culturing (mbt-broth) |
| RU2215039C2 (en) * | 2001-07-31 | 2003-10-27 | Прикаспийский зональный научно-исследовательский ветеринарный институт | Nutrient medium for mycobacterium isolation and growing |
| RU2295561C2 (en) * | 2005-02-15 | 2007-03-20 | Государственное учреждение Якутский научно-исследовательский институт туберкулеза Министерства здравоохранения Республики Саха (Якутия) (ГУ Якутский НИИ туберкулеза МЗ РС (Я)) | Method for production of medium for mycobacterium tuberculosis (mbt) culturing |
| RU2320716C2 (en) * | 2006-04-17 | 2008-03-27 | Государственное научное учреждение Научно-исследовательский ветеринарный институт Нечерноземной зоны РФ Российской академии сельскохозяйственных наук | Solid nutrient medium for mycobacterium culturing |
| RU2322495C2 (en) * | 2006-04-17 | 2008-04-20 | Государственное научное учреждение Научно-исследовательский ветеринарный институт Нечерноземной зоны РФ Российской академии сельскохозяйственных наук | Nutrient medium for culturing mycobacterium and nocardioformous actinomyces |
Non-Patent Citations (2)
| Title |
|---|
| DIFCO MANUAL. BACTO LOWENSTEIN MEDIUM BASE. TENTH Edition, 1984, p. 531-533 * |
| Финкель Е.А., Погребинская Ю.Б. Сухие питательные среды для диагностики туберкулеза. Фрунзе, 1977, 128 р. * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD4030B1 (en) | 2010-04-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |