CN103110968A - Energy-conservation sterilizing method for microbial fermentation - Google Patents
Energy-conservation sterilizing method for microbial fermentation Download PDFInfo
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- CN103110968A CN103110968A CN2013100308408A CN201310030840A CN103110968A CN 103110968 A CN103110968 A CN 103110968A CN 2013100308408 A CN2013100308408 A CN 2013100308408A CN 201310030840 A CN201310030840 A CN 201310030840A CN 103110968 A CN103110968 A CN 103110968A
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Abstract
The invention provides an energy-conservation sterilizing method for microbial fermentation. The energy-conservation sterilizing method for the microbial fermentation comprises the following steps of: carrying out normal vapor sterilization on a high-concentration culture medium; sterilizing water for the culture medium with ozone; and carrying out catalytic decomposition on the residual ozone. The invention provides a method for combining ozone sterilization and vapor sterilization. Shown by actual tests, not only can the sterilization be thoroughly realized, but also the heating time and the cooling time can be reduced; the loss of nutrients is reduced; most impotently, the energy consumption is greatly reduced, and the investment on a vapor supply and cooling water system by factories can be reduced; and the energy consumption is reduced by 60-90% compared with that of a single vapor sterilization manner. The energy-conservation sterilizing method for the microbial fermentation is easily and widely popularized in an industry, and promotes revolutionary advance in a fermentation industry.
Description
Technical field
The invention belongs to the microbial fermentation field, be specifically related to a kind of method of microbial fermentation energy-saving sterilizing.
Background technology
Traditional microorganism fermentation process is all generally the way that adopts steam sterilization, namely with steam, fermentation tank and cultivation is wherein killed more than 20 minutes based on 121 ℃ before inoculation, and then is cooled to fermentation temperature.All nutrient substance and aqueous solvent thereof all will be heated to 121 ℃ and then use again a large amount of cooling water temperatures in other words, because specific heat of water is larger, traditional fermentation technology is the technique of a highly energy-consuming thus, and efficient is lower.Therefore, how to reduce energy consumption is the problem that fermentation engineering teacher make great efforts to solve for many years always, and what have sets about from design, reduces energy consumption by heat exchange, but does not nonetheless also really reach satisfied effect.
Summary of the invention
For the shortcomings and deficiencies that prior art exists, the purpose of this invention is to provide a kind of method of microbial fermentation energy-saving sterilizing that can the decrease energy consumption.
In order to realize above-mentioned technical assignment, the present invention adopts following technical scheme to be achieved:
A kind of method of microbial fermentation energy-saving sterilizing, specifically carry out according to following steps:
Step 1, the material that culture medium is required is used minimum water dissolution in proportion, this ratio adopts formula to require 10%～20% of water consumption, and the mode that adopts the spraying charging is squeezed into culture medium with steam and is increased in the fermentation tank of 120～125 ℃, press steam sterilization and sterilizes;
Step 2 requires remaining water to use ozone sterilization more than 15 minutes in another storage tank culture medium prescription;
Step 3 is after placing 30min with the pressurize in filtrated air of this water, by making residue ozone decomposition in water with the catalytic decomposition of ozone device of steam sterilization;
Step 4, then will be pressed into fermentation tank with filtrated air through the water that the catalytic decomposition of ozone device is removed ozone, the temperature of fermentation tank is down to below 40 ℃;
Step 5 to the culture medium sample analysis in fermentation tank, is determined can enter follow-up fermentation workshop section after the ozone of noresidue in culture medium.
If there is no sugar in the fermentation medium described in step 1, can add a small amount of glucose in the situation that do not affect fermentation, this part glucose can thoroughly decompose the residual drop ozone of water that injects fermentation tank.
Ozone sterilization described in step 2 is to prepare gaseous ozone with ozonator, then passes into from tank bottoms, perhaps first prepares the ozone water solution of high concentration, adds in the water that will sterilize.
The temperature of the ozone sterilization described in step 2 is selected room temperature.
The catalyst that catalytic decomposition of ozone device described in step 3 is selected is the oxide of transition metal, catalyst be shaped as particle diameter greater than the granule of 0.5 millimeter.
The oxide of described transition metal can directly be used, and is perhaps the oxide of carried transition metal, and the carrier of the oxide of this transition metal is active carbon, SiO
2, Al
2O
3Or kieselguhr.
The invention provides a kind of way that ozone sterilization is engaged with steam sterilization, through actual tests, not only sterilize thorough but also shortened temperature fall time, reduced nutraceutical loss, most importantly greatly reduce energy consumption, reduced the input of factory to steam supply, cooling water system, can loss-rate reduce by 60～90% with steam sterilization merely, be very easy in industrial extensive popularization, will promote fermentation industry revolutionary progress occurs.
The specific embodiment
Defer to technique scheme, the present embodiment provides a kind of method of microbial fermentation energy-saving sterilizing, specifically carries out according to following steps:
Step 1, the material that culture medium is required is used minimum water dissolution in proportion, this ratio adopts formula to require 10%～20% of water consumption, and the mode that adopts the spraying charging is squeezed into culture medium with steam and is increased in the fermentation tank of 120～125 ℃, press steam sterilization and sterilizes;
Step 2 requires remaining water to use ozone sterilization more than 15 minutes in another storage tank culture medium prescription;
Step 3 is after placing 30min with the pressurize in filtrated air of this water, by making residue ozone decomposition in water with the catalytic decomposition of ozone device of steam sterilization;
Step 4, then will be pressed into fermentation tank with filtrated air through the water that the catalytic decomposition of ozone device is removed ozone, the temperature of fermentation tank is down to below 40 ℃;
Step 5 to the culture medium sample analysis in fermentation tank, is determined can enter follow-up fermentation workshop section after the ozone of noresidue in culture medium.
If there is no sugar in the fermentation medium described in step 1, can add a small amount of glucose in the situation that do not affect fermentation, this part glucose can thoroughly decompose the residual drop ozone of water that injects fermentation tank.
Ozone sterilization described in step 2 is to prepare gaseous ozone with ozonator, then passes into from tank bottoms, perhaps first prepares the ozone water solution of high concentration, adds in the water that will sterilize.
The temperature of the ozone sterilization described in step 2 is selected room temperature.
The catalyst that catalytic decomposition of ozone device described in step 3 is selected is the oxide of transition metal, catalyst be shaped as particle diameter greater than the granule of 0.5 millimeter.
The oxide of described transition metal can directly be used, and is perhaps the oxide of carried transition metal, and the carrier of the oxide of this transition metal is active carbon, SiO
2, Al
2O
3Or kieselguhr.
Below provide specific embodiments of the invention, need to prove that the present invention is not limited to following specific embodiment, all equivalents of doing on present techniques scheme basis all fall into protection scope of the present invention.
Embodiment:
The 200mL culture medium of packing in the seedlet bottle of 500mL, the seedlet bottle is 118 ℃ in temperature, pressure is steam sterilization 20 minutes under the condition of 0.08Mpa, it is 34 ℃ in cultivation temperature, shaking speed is inoculated and cultured in the shaking table of 120 rev/mins, about 20 hours of incubation time is inoculated into off-the-shelf large seed bottle with cultivating complete bacterium liquid in the seedlet bottle.
The 2000mL culture medium of packing in the large seed bottle of 3000mL, large seed bottle is 121 ℃ in temperature, pressure is steam sterilization 60 minutes under the condition of 0.1Mpa, large seed bottle is 34 ℃ in cultivation temperature, cultivate in the shaking table that shaking speed is 75 rev/mins, about 16 hours of incubation time changes sterilized first class seed pot over to cultivating complete bacterium liquid in large seed bottle.
With the seed culture in the 100L first class seed pot, then the secondary seed tank that changes 500L over to cultivates according to the cultural method of this area, cultivates seed to be changed in fermentation tank at last after completing.In this process, first class seed pot, secondary seed tank, pipeline, carbon source, nitrogenous source, sour tank, alkali tank, material-compound tank and defoamer tank all adopt steam sterilization.
Consisting of of the culture medium that should add in the fermentation tank of 5000 liters: glucose 100kg, Semen Maydis pulp 50kg, sodium hydrogen phosphate 5kg, potassium dihydrogen phosphate 2.5kg, Chile saltpeter 10kg, magnesium sulfate 2.5kg, sodium chloride 2kg, water 2500kg.The pH value of culture medium is 6.8.
Other the outer raw materials that dewater in the culture medium of fermentation tank first dissolve in material-compound tank with 500kg water, adopt the mode of the charging of spraying to squeeze into and be warming up to 121 ℃ of empty fermentation tanks, and at this temperature steam sterilization 30min; Separately culture medium prescription is required remaining 2000kg water to use ozone sterilization more than 15 minutes in another storage tank, after 30min is placed in the pressurize in filtrated air of water after ozone sterilization, with this water by making residue ozone decomposition in water with the catalytic decomposition of ozone device of steam sterilization; To be pressed into fermentation tank with filtrated air through the water that the catalytic decomposition of ozone device is removed ozone again, temperature in fermentation tank is down to below 40 ℃, adjust the temperature to again temperature required, to the culture medium sample analysis in fermentation tank, determine to enter follow-up fermentation workshop section after the ozone of noresidue in culture medium.
From the above, adopt the sterilizing methods in embodiment, only need to be with other raw material rising temperature to 121 in 500kg water and culture medium ℃, remaining 2000kg water only needs room temperature get final product with ozone sterilization, compares with the method that traditional whole culture medium use are steam heated to 121 ℃ of sterilizations, the energy consumption of sterilization reduces greatly, the reduction amplitude reaches 60%～90%, and the while, amount was few because the concentration of the culture medium of steam sterilization is high, spraying charging, sterilization are thoroughly; To inject fermentation tank with the water of ozone sterilization and can reduce rapidly temperature in fermentation tank, and reduce consuming timely, and reduce cooling water amount, and traditional whole culture medium are with the method that is steam heated to 121 ℃ of sterilizations, heat in fermentation tank, time of cooling needs is long.
Claims (6)
1. the method for a microbial fermentation energy-saving sterilizing, is characterized in that, specifically carries out according to following steps:
Step 1, the material that culture medium is required is used minimum water dissolution in proportion, this ratio adopts formula to require 10%～20% of water consumption, and the mode that adopts the spraying charging is squeezed into culture medium with steam and is increased in the fermentation tank of 120～125 ℃, press steam sterilization and sterilizes;
Step 2 requires remaining water to use ozone sterilization more than 15 minutes in another storage tank culture medium prescription;
Step 3 is after placing 30min with the pressurize in filtrated air of this water, by making residue ozone decomposition in water with the catalytic decomposition of ozone device of steam sterilization;
Step 4, then will be pressed into fermentation tank with filtrated air through the water that the catalytic decomposition of ozone device is removed ozone, the temperature of fermentation tank is down to below 40 ℃;
Step 5 to the culture medium sample analysis in fermentation tank, is determined can enter follow-up fermentation workshop section after the ozone of noresidue in culture medium.
2. the method for microbial fermentation energy-saving sterilizing as claimed in claim 1, it is characterized in that, if there is no sugar in the fermentation medium described in step 1, can add a small amount of glucose in the situation that do not affect fermentation, this part glucose can thoroughly decompose the residual drop ozone of water that injects fermentation tank.
3. the method for microbial fermentation energy-saving sterilizing as claimed in claim 1, it is characterized in that, the ozone sterilization described in step 2 is to prepare gaseous ozone with ozonator, then passes into from tank bottoms, perhaps first prepare the ozone water solution of high concentration, add in the water that to sterilize.
4. the method for microbial fermentation energy-saving sterilizing as claimed in claim 1, is characterized in that, the temperature of the ozone sterilization described in step 2 is selected room temperature.
5. the method for microbial fermentation energy-saving sterilizing as claimed in claim 1, is characterized in that, the catalyst that the catalytic decomposition of ozone device described in step 3 is selected is the oxide of transition metal, catalyst be shaped as particle diameter greater than the granule of 0.5 millimeter.
6. the method for microbial fermentation energy-saving sterilizing as claimed in claim 5, is characterized in that, the oxide of described transition metal can directly be used, and is perhaps the oxide of carried transition metal, and the carrier of the oxide of this transition metal is active carbon, SiO
2, Al
2O
3Or kieselguhr.
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| CN201310030840.8A CN103110968B (en) | 2013-01-25 | 2013-01-25 | Energy-conservation sterilizing method for microbial fermentation |
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| CN103110968B CN103110968B (en) | 2015-01-14 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104307007A (en) * | 2014-04-02 | 2015-01-28 | 杨凌乐达生物科技有限公司 | Method for flash sterilization of culture medium for tissue culture seedlings factory production |
| CN112481324A (en) * | 2020-12-30 | 2021-03-12 | 赵兰坤 | Novel amino acid fermentation sterilization process |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1054014A (en) * | 1990-08-22 | 1991-08-28 | 黄振钧 | Ozone removal and separating catalyst and their preparation method and application |
| JP2002369672A (en) * | 2001-06-14 | 2002-12-24 | Yamaya Communications:Kk | Method for sterilizing food by using lactic fermentation liquor |
| CN1426972A (en) * | 2001-12-17 | 2003-07-02 | 李明秀 | Cooling water circulating treatment system and method |
| CN1462799A (en) * | 2002-05-31 | 2003-12-24 | 华北制药集团有限责任公司 | Technique of preparing fermentation culture medium for microbe |
| TW200744982A (en) * | 2003-12-12 | 2007-12-16 | Taichung Distr Agricultural Improvement Station Council Of Agriculture | Paddyrice culture medium and method for preparing microorganisms from the same |
-
2013
- 2013-01-25 CN CN201310030840.8A patent/CN103110968B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1054014A (en) * | 1990-08-22 | 1991-08-28 | 黄振钧 | Ozone removal and separating catalyst and their preparation method and application |
| JP2002369672A (en) * | 2001-06-14 | 2002-12-24 | Yamaya Communications:Kk | Method for sterilizing food by using lactic fermentation liquor |
| CN1426972A (en) * | 2001-12-17 | 2003-07-02 | 李明秀 | Cooling water circulating treatment system and method |
| CN1462799A (en) * | 2002-05-31 | 2003-12-24 | 华北制药集团有限责任公司 | Technique of preparing fermentation culture medium for microbe |
| TW200744982A (en) * | 2003-12-12 | 2007-12-16 | Taichung Distr Agricultural Improvement Station Council Of Agriculture | Paddyrice culture medium and method for preparing microorganisms from the same |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104307007A (en) * | 2014-04-02 | 2015-01-28 | 杨凌乐达生物科技有限公司 | Method for flash sterilization of culture medium for tissue culture seedlings factory production |
| CN104307007B (en) * | 2014-04-02 | 2017-01-18 | 杨凌乐达生物科技有限公司 | Method for flash sterilization of culture medium for tissue culture seedlings factory production |
| CN112481324A (en) * | 2020-12-30 | 2021-03-12 | 赵兰坤 | Novel amino acid fermentation sterilization process |
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| CN103110968B (en) | 2015-01-14 |
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