CN105925491A - Culture medium of filamentous fungi, preparation method of culture medium and culture method of filamentous fungi by culture medium of filamentous fungi - Google Patents
Culture medium of filamentous fungi, preparation method of culture medium and culture method of filamentous fungi by culture medium of filamentous fungi Download PDFInfo
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- CN105925491A CN105925491A CN201610392669.9A CN201610392669A CN105925491A CN 105925491 A CN105925491 A CN 105925491A CN 201610392669 A CN201610392669 A CN 201610392669A CN 105925491 A CN105925491 A CN 105925491A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention relates to a culture medium of filamentous fungi, a preparation method of the culture medium of the filamentous fungi and a culture method of the filamentous fungi by the culture medium of the filamentous fungi. The culture medium comprises a carbon source, a nitrogen source, dipotassium phosphate, heptahydrate magnesium sulfate, ferrous nitrate nonahydrate, zinc nitrate, potassium chloride, magnesium chloride, a pH(Potential of Hydrogen) regulator and water, wherein the carbon source is selected from sucrose, corn starch hydrolysis sugar or a combination of sucrose and corn starch hydrolysis sugar; the nitrogen source is sodium nitrate, the content of the carbon source is 20 to 70g/L, the content of the nitrogen source is 2 to 5g/L, the content of dipotassium phosphate is 1 to 4g/L, the content of heptahydrate magnesium sulfate is 2 to 5g/L, the content of ferrous nitratenonahydrate is 4 to 6g/L, the content of zinc nitrate is 1 to 3g/L, the content of potassium chloride is 3 to 8g/L, the content of magnesium chloride is 3 to 8g/L, and the pH regulator is selected from sulfuric acid, carbonic acid, phosphoric acid or a combination of sulfuric acid, carbonic acid and phosphoric acid. The culture medium disclosed by the invention can fast culture the filamentous fungi with absolute dominant bacteria.
Description
Technical field
The invention belongs to filamentous fungi culture technique field, be specifically related to one and be suitable to cultivate use
In the culture medium of filamentous fungi of the biological treatment of foul gas, the preparation method of this culture medium
And the method utilizing this culture medium culturing filamentous fungi.
Background technology
At present, because having, equipment is simple, cost is low, without two in the biological treatment of foul gas
The features such as secondary pollution and the most concerned.The fast culture of microorganism and domestication are that biological deodorizing sets
The key of received shipment row.
The microbe species of degraded stink substance includes antibacterial and fungus.Conventional biofilter
Middle microorganism is based on antibacterial, therefore it is required that keep moist environment in biofilter.Stench
The removal of gas can be divided into three phases: Organic substance transfers to liquid phase from gas phase, at liquid
Migrate in mutually and be absorbed by the micro-organisms, be degraded by microorganisms.For the pollutant of good water solubility,
Can transfer to from gas phase rapidly the water layer of bacterium surface, and be degraded by antibacterial.But,
Low or the lyophobic dust for dissolubility in water, the water layer of bacterium surface will hinder its mass transfer
Speed, causes treatment effeciency to reduce.Additionally, the acidifying of filter bed and mummification usually make with antibacterial
It it is the treatment effect variation of main biofilter.And the biofilter based on filamentous fungi can
Well to get rid of hydrophobicity foul gas, the fast culture of filamentous fungi and domestication are the most just
It it is the key of this kind of biological deodorizing equipment good deodorizing effect of holding.
Microbiological culture media is the synthetic nutrient for the artificial preparation of the growths such as microorganism
Matter.Microbiological culture media the most all contains carbohydrate, nitrogen substance, inorganic salt (bag
Include trace element) and vitamin and water etc..The microorganism of Different Nutrition type needs to use
Different culture medium.Some culture medium are possibly together with antibiotics and pigment, for single microorganism
Cultivate and identify.
Culture medium is different due to the raw material of preparation, uses and requires difference, and storage and custody aspect
The most slightly different.General culture medium, being heated, after the moisture absorption, is easily contaminated by bacterial or decomposes change
Matter, therefore general culture medium must preserve at protection against the tide, lucifuge, cool place.Some are needed strict
The culture medium (such as tissue culture medium (TCM)) of sterilizing, the storage of long period, it is necessary to be placed on 2-6 DEG C
Refrigerator in.
Common microbiological culture media includes:
1) sabouraud medium
The component of this culture medium includes peptone 10g；Agar 20g；Maltose 40g；Water
1000ml；
The manufacture method of this culture medium is: after first peptone, agar being added water, heating, no
Disconnected stirring, after agar dissolves, adds 40g maltose (or glucose), stirring, makes it
Dissolve, then subpackage, sterilizing.
2) potato sugar agar culture medium
The component of this culture medium includes Rhizoma Solani tuber osi 200g；Agar 10g；Sugar 20g；Water 1000ml.
The manufacture method of this culture medium is: Rhizoma Solani tuber osi is cleaned peeling, takes 200g and be cut into small pieces,
Add water 1000ml, after boiling half an hour, supplies moisture, adds 10g agar in filtrate,
Boil sugaring 20g after dissolving, supply moisture, subpackage, sterilizing, standby.The pH of culture medium
Value is transferred to 7.2～7.4.
3) bean sprouts medium
The component of this culture medium includes Semen Glycines Germinatus 100g；Agar 15g；Glucose 20g；Water
1000ml。
The manufacture method of this culture medium is: cleans Semen Glycines Germinatus, adds water boil 30 minutes, use yarn
Cloth filters, and adds agar in filtrate, puts into glucose after heating for dissolving, and stirring makes it dissolve,
Supply moisture to 1000ml, subpackage, sterilizing.
4) Semen Pisi sativi agar culture medium
The component of this culture medium includes Semen Pisi sativi 100g；Agar 25g；Water 1000ml.
The manufacture method of this culture medium is: takes the dry Semen Pisi sativi of 100g and adds water, boils 1 hour, uses
After filtered through gauze, filtrate adds agar, boils dissolving, subpackage, sterilizing.
These common microbiological culture medias carry out filamentous fungi cultivate time, filamentous fungi
The speed of growth is relatively slow, and other microorganisms such as antibacterial etc. the most usually can pollute, thus
Affect the extensive fast-growth of filamentous fungi.
Summary of the invention
In place of the deficiencies in the prior art, it is an object of the invention to provide one can fast culture go out
There is the fluid medium of the filamentous fungi of absolute advantages strain.Filamentous fungi can be top spore head
Spore is mould, aspergillus wentii, penicillium froquentans or paecilomyces varioti.
It is a further object of the present invention to provide preparation method and the utilization of a kind of filamentous fungi culture medium
The method of filamentous fungi culture medium culturing filamentous fungi.
For achieving the above object, technical scheme is summarized as follows:
A kind of filamentous fungi culture medium, comprising: carbon source, nitrogen source, dipotassium hydrogen phosphate, seven water sulfur
Acid magnesium, nine water ferrous nitrate, zinc nitrate, potassium chloride, magnesium chloride, pH adjusting agent and water；
Wherein, described carbon source is selected from sucrose, corn starch hydrolysis sugar or a combination thereof；Described nitrogen
Source is sodium nitrate, and the content of described carbon source is 20-70g/L, and the content in described nitrogen source is
2-5g/L, the content of described dipotassium hydrogen phosphate is 1-4g/L, the content of described Magnesium sulfate heptahydrate
For 2-5g/L, the content of described nine water ferrous nitrate is 4-6g/L, the content of described zinc nitrate
For 1-3g/L, the content of described potassium chloride is 3-8g/L, and the content of described magnesium chloride is
3-8g/L, described pH adjusting agent is selected from sulphuric acid, carbonic acid, phosphoric acid or a combination thereof.
Preferably, described filamentous fungi culture medium, wherein, described carbon source is sucrose, described carbon
The content in source is 20-50g/L.
Preferably, described filamentous fungi culture medium, wherein, described carbon source is corn starch hydrolysis
Sugar, the content of described carbon source is 40-70g/L.
Preferably, described filamentous fungi culture medium, wherein, described filamentous fungi culture medium
PH is 3.5-4.5.
A kind of preparation method of the filamentous fungi culture medium as according to any one of such scheme,
Comprising: weigh in proportion water, carbon source, nitrogen source, dipotassium hydrogen phosphate, Magnesium sulfate heptahydrate,
Nine water ferrous nitrate, zinc nitrate, potassium chloride and magnesium chloride, add in reaction vessel, fully
After shaken well, add pH adjusting agent by pH regulator to 3.5-4.5, high temperature sterilize, i.e.
Obtain filamentous fungi culture medium.
Preferably, the preparation method of described filamentous fungi culture medium, wherein, described pH
Regulator is sulphuric acid or concentration is the phosphate aqueous solution of 10wt%-50wt%.
Preferably, the preparation method of described filamentous fungi culture medium, wherein, described sulfur
Acid concentration is 10wt%-25wt%.
The cultural method of a kind of filamentous fungi, comprising: take the fresh inclined-plane training of filamentous fungi
Support thing, be prepared as bacterium solution, be seeded to the filamentous fungi as described in any one in such scheme
In culture medium, constant temperature culture 5-10 days at 27 DEG C-29 DEG C, obtain filamentous fungi.
The invention has the beneficial effects as follows: the culture medium of this case is particularly suitable for the biology of foul gas
The filamentous fungi such as cephalosporium acremonium, aspergillus wentii of process, penicillium froquentans, Wan Shi intend green grass or young crops
The fast culture of mould grade, and other fungus outside filamentous fungi and absolutely can be suppressed simultaneously
The growth of most of antibacterials, therefore, it can turn out the filamentous fungi into absolute advantages strain；
The deodorization filamentous fungi cultivated by this case can quickly be seeded to deodorization bioreactor, tool
There is the good effect removing hydrophobicity foul gas；Additionally, the culture medium dilution of this case
The nutritional solution of deodorization bioreactor it is also used as after 5-10 times；Or removing carbon source or nitrogen
The nutritional solution of deodorization bioreactor is can act also as behind source or sulfur component.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, to make those skilled in the art join
Book word can be implemented according to this as directed.
Embodiment 1
The present embodiment provides a kind of filamentous fungi culture medium, and its preparation method is as follows: select resistance to
High temperature 2000 milliliters can be jumped a queue glass container, the most accurately measures 1000 milliliters of pure water and adds
Enter, the most respectively precise sucrose 45 grams, sodium nitrate 3 grams, dipotassium hydrogen phosphate 2 grams,
Magnesium sulfate heptahydrate 4 grams, nine water ferrous nitrate 5 grams, zinc nitrate 2 grams, 6 grams of potassium chloride,
5 grams of magnesium chloride, is sequentially added into glass container, abundant shaken well, adds 30wt% phosphoric acid
Aqueous solution adjustment pH value is to 4.1, and ventilative cotton thread plug seals glass container, obtains after high temperature sterilize
Obtain filamentous fungi culture medium.
Take the fresh slant culture system of paecilomyces varioti (Paecilomyces variotii)
Standby one-tenth bacterium solution, after being seeded to above-mentioned culture medium, 28 DEG C of constant temperature culture tests.After cultivating 5 days
The quantity measuring its brood body is 4*109CFU/l。
Embodiment 2
The present embodiment provides a kind of filamentous fungi culture medium, and its preparation method is as follows: select resistance to
High temperature 2000 milliliters can be jumped a queue glass container, the most accurately measures 1000 milliliters of pure water and adds
Enter, the most respectively precise sucrose 30 grams, sodium nitrate 4 grams, dipotassium hydrogen phosphate 3 grams,
Magnesium sulfate heptahydrate 4 grams, nine water ferrous nitrate 5 grams, zinc nitrate 2 grams, 5 grams of potassium chloride,
5 grams of magnesium chloride, is sequentially added into glass container, abundant shaken well, adds 30wt% phosphoric acid
Aqueous solution adjustment pH value is to 4.1, and ventilative cotton thread plug seals glass container, obtains after high temperature sterilize
Obtain filamentous fungi culture medium.
Take the fresh slant culture system of paecilomyces varioti (Paecilomyces variotii)
Standby one-tenth bacterium solution, after being seeded to above-mentioned culture medium, 28 DEG C of constant temperature culture tests.After cultivating 5 days
The quantity measuring its brood body is 3.9 × 109CFU/l。
Embodiment 3
The present embodiment provides a kind of filamentous fungi culture medium, and its preparation method is as follows: select resistance to
High temperature 2000 milliliters can be jumped a queue glass container, the most accurately measures 1000 milliliters of pure water and adds
Enter, the most respectively precise sucrose 20 grams, sodium nitrate 5 grams, dipotassium hydrogen phosphate 1 gram,
Magnesium sulfate heptahydrate 3 grams, nine water ferrous nitrate 5 grams, zinc nitrate 2 grams, 3 grams of potassium chloride,
6 grams of magnesium chloride, is sequentially added into glass container, abundant shaken well, adds the dilute sulfur of 20wt%
Acid adjustment pH value is to 4.1, and ventilative cotton thread plug seals glass container, obtains silk after high temperature sterilize
Shape fungi culture medium.
Take the fresh slant culture system of paecilomyces varioti (Paecilomyces variotii)
Standby one-tenth bacterium solution, after being seeded to above-mentioned culture medium, 28 DEG C of constant temperature culture tests.After cultivating 5 days
The quantity measuring its brood body is 3.9 × 109CFU/l。
Embodiment 4
The present embodiment provides a kind of filamentous fungi culture medium, and its preparation method is as follows: select resistance to
High temperature 2000 milliliters can be jumped a queue glass container, the most accurately measures 1000 milliliters of pure water and adds
Enter, the most respectively precise sucrose 20 grams, sodium nitrate 5 grams, dipotassium hydrogen phosphate 1 gram,
Magnesium sulfate heptahydrate 3 grams, nine water ferrous nitrate 5 grams, zinc nitrate 2 grams, 3 grams of potassium chloride,
6 grams of magnesium chloride, is sequentially added into glass container, abundant shaken well, adds the dilute sulfur of 20wt%
Acid adjustment pH value is to 4.5, and ventilative cotton thread plug seals glass container, obtains silk after high temperature sterilize
Shape fungi culture medium.
Take the fresh slant culture system of paecilomyces varioti (Paecilomyces variotii)
Standby one-tenth bacterium solution, after being seeded to above-mentioned culture medium, 28 DEG C of constant temperature culture tests.After cultivating 5 days
The quantity measuring its brood body is 3.7 × 109CFU/l。
Embodiment 5
The present embodiment provides a kind of filamentous fungi culture medium, and its preparation method is as follows: select resistance to
High temperature 2000 milliliters can be jumped a queue glass container, the most accurately measures 1000 milliliters of pure water and adds
Enter, the most respectively precise sucrose 20 grams, sodium nitrate 5 grams, dipotassium hydrogen phosphate 1 gram,
Magnesium sulfate heptahydrate 3 grams, nine water ferrous nitrate 5 grams, zinc nitrate 2 grams, 3 grams of potassium chloride,
6 grams of magnesium chloride, is sequentially added into glass container, abundant shaken well, adds the dilute sulfur of 20wt%
Acid adjustment pH value is to 3.5, and ventilative cotton thread plug seals glass container, obtains silk after high temperature sterilize
Shape fungi culture medium.
Take the fresh slant culture system of paecilomyces varioti (Paecilomyces variotii)
Standby one-tenth bacterium solution, after being seeded to above-mentioned culture medium, 28 DEG C of constant temperature culture tests.After cultivating 5 days
The quantity measuring its brood body is 3.5 × 109CFU/l。
Embodiment 6
The present embodiment provides a kind of filamentous fungi culture medium, and its preparation method is as follows: select resistance to
High temperature 2000 milliliters can be jumped a queue glass container, the most accurately measures 1000 milliliters of pure water and adds
Enter, prepare the most respectively to weigh corn starch hydrolysis sugar 60 grams, sodium nitrate 3 grams, phosphoric acid hydrogen
Dipotassium 2 grams, Magnesium sulfate heptahydrate 4 grams, nine water ferrous nitrate 5 grams, zinc nitrate 2 grams, chlorine
Change 6 grams of potassium, 5 grams of magnesium chloride, be sequentially added into glass container, abundant shaken well, 50wt%
Phosphoric acid adjustment pH value is to 4.1, and ventilative cotton thread plug seals glass container, obtains after high temperature sterilize
Filamentous fungi culture medium.
Take penicillium froquentans (Penicillium frequentans), aspergillus wentii
Mixing of (Aspergillus wentii), bacillus subtilis (Bacillus subtilis) etc.
Closing bacterium solution and be seeded to above-mentioned culture medium, 28 DEG C of constant temperature culture are observed.Test confirms the most now penicillium sp
The filamentous fungis such as bacterium (Penicillium frequentans) are rapid in the growth of this culture medium,
Become dominant bacteria；And the antibacterials such as bacillus subtilis (Bacillus subtilis) are in this cultivation
The growth of base is suppressed.During cultivation, (7d) is not detected by bacillus subtilis bacterium colony, and blue or green
The quantity of the brood body of mycete and aspergillus wentii respectively reaches 3.8 × 109More than CFU/l.
Embodiment 7
The present embodiment provides a kind of filamentous fungi culture medium, and its preparation method is as follows: select resistance to
High temperature 2000 milliliters can be jumped a queue glass container, the most accurately measures 1000 milliliters of pure water and adds
Enter, prepare the most respectively to weigh corn starch hydrolysis sugar 40 grams, sodium nitrate 4 grams, phosphoric acid hydrogen
Dipotassium 4 grams, Magnesium sulfate heptahydrate 2 grams, nine water ferrous nitrate 5 grams, zinc nitrate 2 grams, chlorine
Change 6 grams of potassium, 5 grams of magnesium chloride, be sequentially added into glass container, abundant shaken well, phosphorate
Acid adjustment pH value is to 4.1, and ventilative cotton thread plug seals glass container, obtains silk after high temperature sterilize
Shape fungi culture medium.
Take penicillium froquentans (Penicillium frequentans), aspergillus wentii
Mixing of (Aspergillus wentii), bacillus subtilis (Bacillus subtilis) etc.
Closing bacterium solution and be seeded to above-mentioned culture medium, 28 DEG C of constant temperature culture are observed.Test confirms the most now penicillium sp
The filamentous fungis such as bacterium (Penicillium frequentans) are rapid in the growth of this culture medium,
Become dominant bacteria；And the antibacterials such as bacillus subtilis (Bacillus subtilis) are in this cultivation
The growth of base is suppressed.During cultivation, (7d) is not detected by bacillus subtilis bacterium colony, and blue or green
The quantity of the brood body of mycete and aspergillus wentii respectively reaches 3.8 × 109More than CFU/l.
Embodiment 8
The present embodiment provides a kind of filamentous fungi culture medium, and its preparation method is as follows: select resistance to
High temperature 2000 milliliters can be jumped a queue glass container, the most accurately measures 1000 milliliters of pure water and adds
Enter, prepare the most respectively to weigh corn starch hydrolysis sugar 40 grams, sodium nitrate 4 grams, phosphoric acid hydrogen
Dipotassium 4 grams, Magnesium sulfate heptahydrate 2 grams, nine water ferrous nitrate 5 grams, zinc nitrate 2 grams, chlorine
Change 6 grams of potassium, 5 grams of magnesium chloride, be sequentially added into glass container, abundant shaken well, phosphorate
Acid adjustment pH value is to 4.1, and ventilative cotton thread plug seals glass container, obtains silk after high temperature sterilize
Shape fungi culture medium.
Take penicillium froquentans (Penicillium frequentans), aspergillus wentii
Mixing of (Aspergillus wentii), bacillus subtilis (Bacillus subtilis) etc.
Closing bacterium solution and be seeded to above-mentioned culture medium, 28 DEG C of constant temperature culture are observed.Test confirms the most now penicillium sp
The filamentous fungis such as bacterium (Penicillium frequentans) are rapid in the growth of this culture medium,
Become dominant bacteria；And the antibacterials such as bacillus subtilis (Bacillus subtilis) are in this cultivation
The growth of base is suppressed.During cultivation, (7d) is not detected by bacillus subtilis bacterium colony, and blue or green
The quantity of the brood body of mycete and aspergillus wentii respectively reaches 3.8 × 109More than CFU/l.
Although embodiment of the present invention are disclosed as above, but it is not restricted to description and embodiment party
Listed utilization in formula, it can be applied to various applicable the field of the invention completely, for being familiar with ability
For the personnel in territory, be easily achieved other amendment, therefore without departing substantially from claim and etc. homotype
Enclosing under limited general concept, the present invention is not limited to specific details and shown here embodiment.
Claims (8)
1. a filamentous fungi culture medium, it is characterised in that including: carbon source, nitrogen source, phosphoric acid hydrogen
Dipotassium, Magnesium sulfate heptahydrate, nine water ferrous nitrate, zinc nitrate, potassium chloride, magnesium chloride, pH
Regulator and water；
Wherein, described carbon source is selected from sucrose, corn starch hydrolysis sugar or a combination thereof；Described nitrogen
Source is sodium nitrate, and the content of described carbon source is 20-70g/L, and the content in described nitrogen source is
2-5g/L, the content of described dipotassium hydrogen phosphate is 1-4g/L, the content of described Magnesium sulfate heptahydrate
For 2-5g/L, the content of described nine water ferrous nitrate is 4-6g/L, the content of described zinc nitrate
For 1-3g/L, the content of described potassium chloride is 3-8g/L, and the content of described magnesium chloride is
3-8g/L, described pH adjusting agent is selected from sulphuric acid, carbonic acid, phosphoric acid or a combination thereof.
Filamentous fungi culture medium the most according to claim 1, it is characterised in that described carbon source is
Sucrose, the content of described carbon source is 20-50g/L.
Filamentous fungi culture medium the most according to claim 1, it is characterised in that described carbon source is
Corn starch hydrolysis sugar, the content of described carbon source is 40-70g/L.
Filamentous fungi culture medium the most according to claim 1, it is characterised in that described thread very
The pH of bacterium culture medium is 3.5-4.5.
5. the preparation of the filamentous fungi culture medium as according to any one of claim 1-4
Method, it is characterised in that weigh in proportion water, carbon source, nitrogen source, dipotassium hydrogen phosphate, seven
Water magnesium sulfate, nine water ferrous nitrate, zinc nitrate, potassium chloride and magnesium chloride, add reaction and hold
In device, after abundant shaken well, add pH adjusting agent by pH regulator to 3.5-4.5, height
Temperature sterilizing, obtains filamentous fungi culture medium.
The preparation method of filamentous fungi culture medium the most according to claim 5, its feature
It is: described pH adjusting agent is sulphuric acid or concentration is the phosphate aqueous solution of 10wt%-50wt%.
The preparation method of filamentous fungi culture medium the most according to claim 6, its feature
It is: described sulfuric acid concentration is 10wt%-25wt%.
8. the cultural method of a filamentous fungi, it is characterised in that take the fresh of filamentous fungi
Slant culture, is prepared as bacterium solution, is seeded to as described in any one in claim 1-4
Filamentous fungi culture medium in, constant temperature culture 5-10 days at 27 DEG C-29 DEG C, obtain thread
Fungus.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107902849A (en) * | 2017-12-24 | 2018-04-13 | 苏州市海魄洁净环境工程有限公司 | Constant temperature water supply system |
| CN108144435A (en) * | 2017-12-24 | 2018-06-12 | 苏州市海魄洁净环境工程有限公司 | Air purification composite bioreactor |
| CN112625999A (en) * | 2020-12-30 | 2021-04-09 | 嘉必优生物技术（武汉）股份有限公司 | Method for domesticating filamentous fungi through continuous fermentation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757902A (en) * | 2012-07-20 | 2012-10-31 | 江苏苏净集团有限公司 | Filamentous fungus culture medium, method for preparing same, and method for culturing filamentous fungi utilizing culture medium |
| CN104031841A (en) * | 2013-03-07 | 2014-09-10 | 江苏苏净集团有限公司 | Filamentous fungus culturing medium, preparation method thereof, and method for culturing filamentous fungi by using culturing medium |
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2016
- 2016-06-06 CN CN201610392669.9A patent/CN105925491A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757902A (en) * | 2012-07-20 | 2012-10-31 | 江苏苏净集团有限公司 | Filamentous fungus culture medium, method for preparing same, and method for culturing filamentous fungi utilizing culture medium |
| CN104031841A (en) * | 2013-03-07 | 2014-09-10 | 江苏苏净集团有限公司 | Filamentous fungus culturing medium, preparation method thereof, and method for culturing filamentous fungi by using culturing medium |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107902849A (en) * | 2017-12-24 | 2018-04-13 | 苏州市海魄洁净环境工程有限公司 | Constant temperature water supply system |
| CN108144435A (en) * | 2017-12-24 | 2018-06-12 | 苏州市海魄洁净环境工程有限公司 | Air purification composite bioreactor |
| CN112625999A (en) * | 2020-12-30 | 2021-04-09 | 嘉必优生物技术（武汉）股份有限公司 | Method for domesticating filamentous fungi through continuous fermentation |
| CN112625999B (en) * | 2020-12-30 | 2022-08-09 | 嘉必优生物技术（武汉）股份有限公司 | Method for domesticating filamentous fungi through continuous fermentation |
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