CN104307007A - Method for flash sterilization of culture medium for tissue culture seedlings factory production - Google Patents

Abstract

The invention discloses a method for flash sterilization of a culture medium for tissue culture seedlings factory production. The method comprises the steps of dissolving materials required by the culture medium with water according to proportions; dropwise adding ozone water; sub-packaging; putting sub-packaged culture medium into a sterilization tank F with inlet valves K1-K3 and an outlet valve K4; closing the outlet valve K4; introducing ozone generated by an ozone generator D into the sterilization tank; introducing water vapor generated by an atomizer E into the sterilization tank; controlling an ozone concentration at 0.5-30 ppm, a temperature at 5-70 DEG C, a humidity at 20-95%, a pressure at 0.01-0.1 MPa and a time at 1-40 minutes; opening the outlet valve K4; and introducing purified air from the inlet valve K1 to purge and replace residual ozone in the sterilization tank F to an ozone decomposer G to decompose the residual ozone. The method provided by the invention is thorough in sterilization, shortens time of heating and cooling, and greatly reduces energy consumption.

Description

A kind of method of factorial praluction tissue cultured seedling culture medium quick sterilization

Technical field

The invention belongs to biological field, be specifically related to a kind of method of factorial praluction tissue cultured seedling culture medium quick sterilization.

Background technology

Adopt factorial praluction tissue cultured seedling to drastically increase reproduction speed and the efficiency of seedling, be widely used in various woody, herbal plantation.In industrialized tissue culture Seedling incubation, a very crucial step is medium sterilization, this directly affects the survival rate of immature Seedling, the general practice is that the culture medium prepared to be contained in a kind of special vial at steam pressure be 135kpa, temperature is sterilizing at 121 DEG C, the thorough sterilizing of culture medium can be ensured although do like this, also there are some shortcomings.One is that energy consumption is high, and sterilizing heats up slow, terminates rear temperature fall time long, affects production efficiency; Two is because adopt high temperature sterilize, and container used is general all with resistant to elevated temperatures containers such as glass, easily broken, not easily transports for long-distance, is unfavorable for recycling.Just because of there being these shortcomings, also the serialization of tissue cultured seedling production process just can not be made.Therefore, how to reduce energy consumption, overcome above-mentioned shortcoming is the problem that group training engineers makes great efforts to solve for many years always, and what have sets about from management, is raised the efficiency by scheduling of production, but does not nonetheless also really reach satisfied effect.

Summary of the invention

For the shortcomings and deficiencies that prior art exists, the object of this invention is to provide a kind of method that significantly can reduce energy consumption, organize the factorial praluction tissue cultured seedling culture medium quick sterilization of culture container employing recyclable materials.

In order to realize above-mentioned technical assignment, the present invention adopts following technical scheme to be achieved:

A method for factorial praluction tissue cultured seedling culture medium quick sterilization, specifically carry out according to following steps:

1) material needed for culture medium is used water dissolution in proportion, then be added dropwise to 0.4 ~ 5.0mg/L Ozone Water of total water consumption 5% ~ 15% amount, standardize solution is divided in transparent plastic bottle;

2) plastic bottle containing culture medium is put into the sterilization tank F with sealed door, intake valve K1 ~ K3, air outlet valve K4, close sealed door, air outlet valve K4, intake valve K1, K2, K3 are connected with ozonator D, nebulizer E, air cleaner C by pipeline successively, and air outlet valve K4 is connected with ozonolysis equipment G by pipeline; Pass in sterilization tank by the ozone that ozonator D produces, pass in sterilization tank by the steam that nebulizer E produces, control ozone concentration 0.5 ~ 30 ppm, temperature 5 ~ 70 DEG C, humidity 20 ~ 95%, pressure is at 0.01 ~ 0.1MPa, and the time was at 1 ~ 40 minute;

3) open air outlet valve K4, pass into the air of purification from intake valve K1, purge the air of residual ozone in displacement sterilization tank F;

4) ozone-containing air that air outlet valve K4 discharges is passed into ozonolysis equipment G, decompose remaining ozone;

5) sterilizing directly can enter subsequent processing after terminating.

The invention provides a kind of method of factorial praluction tissue cultured seedling culture medium quick sterilization, through actual tests, not only sterilizing thoroughly but also shorten temperature fall time, decrease nutraceutical loss, most importantly energy consumption is greatly reduced, the material that the degradables such as polyester can be used to recycle owing to adopting room temperature sterilizing is as the container of culture medium, reduce production cost, improve production efficiency, be very easy to extensively promote on tissue cultured seedling is produced, tissue cultured seedling factorial praluction will be promoted revolutionary progress occurs.

Accompanying drawing explanation

Fig. 1 is factorial praluction tissue cultured seedling culture medium Quick sterilizing device structural representation.

Fig. 2 is culture medium agitating device structure chart.

Below in conjunction with drawings and Examples, explanation is further elaborated to technical scheme of the present invention.

Detailed description of the invention

Defer to technique scheme, the present embodiment provides a kind of method of factorial praluction tissue cultured seedling culture medium quick sterilization, specifically carries out according to following steps:

embodiment 1

1) in the main batching kettle A of the band stirring of 1000 liters, 700kg water, 0.4kg ammonium nitrate, 0.5kg potassium nitrate, 0.1kg glycine, 0.05kg malic acid, 0.2kg inositol, 0.5kg peptone, 0.2kg potassium dihydrogen phosphate is added, heated and stirred to 45 ~ 55 DEG C, add 1.0kg activated carbon until completely dissolved.Adding add 100kg water, 4.0kg agar, 20.0kg sucrose heating for dissolving in auxiliary ingredients still after becomes owner of in batching kettle again, adds the slurry made with 100.0kg water, 40kg Fructus Musae, 20kg Rhizoma Solani tuber osi after main batching kettle stirs.Add 100.0kg Ozone Water (4.0mg/L) after stirring again, to stir after 5 minutes cooling and fixed molten with 80ml-100ml, be divided in polyester bottles.

2) the polyester bottles loading one installed above-mentioned point has sealed door, into and out of in the sterilization tank of air valve, closes sealed door, air outlet valve.Pass in sterilization tank by the ozone that ozonator produces, control ozone concentration 5ppm, temperature controls at 30 DEG C, humidity 45 ~ 55%, and pressure is at 0.01MPa, and the time was at 15 minutes.

3) open air outlet valve, the air passing into purification from air inlet purges ozone-containing air remaining displacement sterilization tank, the ozone-containing air that gas outlet is discharged is passed into ozonolysis equipment simultaneously and decomposes remaining ozone, enter subsequent processing after to be replaced.

The catalyst that described catalytic decomposition of ozone device is selected is the oxide of transition metal, and the shape of catalyst is the granule that particle diameter is greater than 0.5 millimeter.

The oxide of described transition metal can directly be applied, or is the oxide of carried transition metal, and the carrier of the oxide of this transition metal is active carbon, SiO2, Al2O3 or kieselguhr.

embodiment 2

1) in the main batching kettle A of the band stirring of 1000 liters, 720kg water, 0.4kg ammonium nitrate, 0.5kg potassium nitrate, 0.1kg glycine, 0.05kg malic acid, 0.2kg inositol, 0.5kg peptone, 0.2kg potassium dihydrogen phosphate is added, heated and stirred to 45 ~ 55 DEG C, add 1.0kg activated carbon until completely dissolved.Adding add 100kg water, 4.0kg agar, 20.0kg sucrose heating for dissolving in auxiliary ingredients still after becomes owner of in batching kettle again, adds the slurry made with 100.0kg water, 40kg Fructus Musae, 20kg Rhizoma Solani tuber osi after main batching kettle stirs.Add 80.0kg Ozone Water (4.5mg/L) after stirring again, to stir after 5 minutes cooling and fixed molten with 80ml-100ml, be divided in polyester bottles.

2) the polyester bottles loading one installed above-mentioned point has sealed door, into and out of in the sterilization tank of air valve, closes sealed door, air outlet valve.Pass in sterilization tank by the ozone that ozonator produces, control ozone concentration 3ppm, temperature controls at 30 DEG C, humidity 45 ~ 55%, and pressure is at 0.01MPa, and the time was at 30 minutes.

3) open air outlet valve, the air passing into purification from air inlet purges ozone-containing air remaining displacement sterilization tank, the ozone-containing air that gas outlet is discharged is passed into ozonolysis equipment simultaneously and decomposes remaining ozone, enter subsequent processing after to be replaced.

embodiment 3

1) in the main batching kettle A of the band stirring of 1000 liters, 750kg water, 0.4kg ammonium nitrate, 0.5kg potassium nitrate, 0.1kg glycine, 0.05kg malic acid, 0.2kg inositol, 0.5kg peptone, 0.2kg potassium dihydrogen phosphate is added, heated and stirred to 45 ~ 55 DEG C, add 1.0kg activated carbon until completely dissolved.Adding add 100kg water, 4.0kg agar, 20.0kg sucrose heating for dissolving in auxiliary ingredients still after becomes owner of in batching kettle again, adds the slurry made with 100.0kg water, 40kg Fructus Musae, 20kg Rhizoma Solani tuber osi after main batching kettle stirs.Add 50.0kg Ozone Water (5.0mg/L) after stirring again, to stir after 5 minutes cooling and fixed molten with 80ml-100ml, be divided in polyester bottles.

2) the polyester bottles loading one installed above-mentioned point has sealed door, into and out of in the sterilization tank of air valve, closes sealed door, air outlet valve.Pass in sterilization tank by the ozone that ozonator produces, control ozone concentration 5ppm, temperature controls at 30 DEG C, humidity 45 ~ 55%, and pressure is at 0.01MPa, and the time was at 20 minutes.

3) open air outlet valve, the air passing into purification from air inlet purges ozone-containing air remaining displacement sterilization tank, the ozone-containing air that gas outlet is discharged is passed into ozonolysis equipment simultaneously and decomposes remaining ozone, enter subsequent processing after to be replaced.

From the above, adopt the sterilizing methods in embodiment, only need warm for culture medium dissolving, sterilizing is at room temperature carried out, with traditional whole culture medium with compared with the method being steam heated to 121 DEG C of sterilizings, the energy consumption of sterilizing reduces greatly, and reduction amplitude reaches 60% ~ 90%, simultaneously because sterilising temp is low, the polyester bottles of reproducible utilization can be adopted to replace frangible vial, and do not need long-time cooling, directly enter next procedure, be conducive to the serialization of producing.

Claims (1)

1. a method for factorial praluction tissue cultured seedling culture medium quick sterilization, is characterized in that, specifically carries out according to following steps:

1) material needed for culture medium is used water dissolution in proportion, then be added dropwise to 0.4 ~ 5.0mg/L Ozone Water of total water consumption 5% ~ 15% amount, standardize solution is divided in transparent plastic bottle;

2) plastic bottle containing culture medium is put into the sterilization tank F with sealed door, intake valve K1 ~ K3, air outlet valve K4, close sealed door, air outlet valve K4, intake valve K1, K2, K3 are connected with ozonator D, nebulizer E, air cleaner C by pipeline successively, and air outlet valve K4 is connected with ozonolysis equipment G by pipeline; Pass in sterilization tank by the ozone that ozonator D produces, pass in sterilization tank by the steam that nebulizer E produces, control ozone concentration 0.5 ~ 30 ppm, temperature 5 ~ 70 DEG C, humidity 20 ~ 95%, pressure is at 0.01 ~ 0.1MPa, and the time was at 1 ~ 40 minute;

3) open air outlet valve K4, pass into the air of purification from intake valve K1, purge the air of residual ozone in displacement sterilization tank F;

4) ozone-containing air that air outlet valve K4 discharges is passed into ozonolysis equipment G, decompose remaining ozone;

5) sterilizing directly can enter subsequent processing after terminating.