CN103110968B - Energy-conservation sterilizing method for microbial fermentation - Google Patents
Energy-conservation sterilizing method for microbial fermentation Download PDFInfo
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- CN103110968B CN103110968B CN201310030840.8A CN201310030840A CN103110968B CN 103110968 B CN103110968 B CN 103110968B CN 201310030840 A CN201310030840 A CN 201310030840A CN 103110968 B CN103110968 B CN 103110968B
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Abstract
The invention provides an energy-conservation sterilizing method for microbial fermentation. The energy-conservation sterilizing method for the microbial fermentation comprises the following steps of: carrying out normal vapor sterilization on a high-concentration culture medium; sterilizing water for the culture medium with ozone; and carrying out catalytic decomposition on the residual ozone. The invention provides a method for combining ozone sterilization and vapor sterilization. Shown by actual tests, not only can the sterilization be thoroughly realized, but also the heating time and the cooling time can be reduced; the loss of nutrients is reduced; most impotently, the energy consumption is greatly reduced, and the investment on a vapor supply and cooling water system by factories can be reduced; and the energy consumption is reduced by 60-90% compared with that of a single vapor sterilization manner. The energy-conservation sterilizing method for the microbial fermentation is easily and widely popularized in an industry, and promotes revolutionary advance in a fermentation industry.
Description
Technical field
The invention belongs to field of microbial fermentation, be specifically related to a kind of method of fermentable energy-saving sterilizing.
Background technology
Traditional microorganism fermentation process is all generally the way adopting steam sterilization, namely before inoculation, with steam, fermentation tank and cultivation is wherein killed more than 20 minutes based on 121 DEG C, and then is cooled to fermentation temperature.Nutrient substance all in other words and aqueous solvent thereof all will be heated to 121 DEG C and then use a large amount of cooling water temperatures again, because specific heat of water is larger, fermentation technology traditional is thus the technique of a highly energy-consuming, and efficiency comparison is low.Therefore, how to reduce energy consumption is the problem that fermentation engineering teacher make great efforts to solve for many years always, and what have sets about from design, reduces energy consumption, but nonetheless also really do not reach satisfied effect by heat exchange.
Summary of the invention
For the shortcomings and deficiencies that prior art exists, the object of this invention is to provide a kind of method that significantly can reduce the fermentable energy-saving sterilizing of energy consumption.
In order to realize above-mentioned technical assignment, the present invention adopts following technical scheme to be achieved:
A method for fermentable energy-saving sterilizing, specifically carry out according to following steps:
Step one, by the material needed for culture medium in proportion with minimum water dissolution, this ratio adopts 10% ~ 20% of recipe requirements water consumption, adopts the mode of spraying charging culture medium to be squeezed into and is increased in the fermentation tank of 120 ~ 125 DEG C, by steam sterilization sterilizing with steam;
Step 2, requires remaining water in another storage tank with ozone sterilization more than 15 minutes by culture medium prescription;
Step 3, after 30min is placed in pressurize in filtrated air by this water, makes the residue ozone in water decompose by the catalytic decomposition of ozone device with steam sterilization;
Step 4, then the water filtrated air through catalytic decomposition of ozone device removing ozone is pressed into fermentation tank, the temperature of fermentation tank is down to less than 40 DEG C;
Step 5, to the culture medium sample analysis in fermentation tank, can enter follow-up fermentation workshop section after determining the ozone of noresidue in culture medium.
If there is no sugar in the fermentation medium described in step one, a small amount of glucose can be added when not affecting fermentation, the drop ozone that the water that this part glucose thoroughly can decompose injection fermentation tank remains.
Ozone sterilization described in step 2 prepares gaseous ozone with ozonator, then passes into from tank bottoms, or first prepare the ozone water solution of high concentration, add and want in the water of sterilizing.
The thermal creep stress room temperature of the ozone sterilization described in step 2.
The catalyst that catalytic decomposition of ozone device described in step 3 is selected is the oxide of transition metal, and the shape of catalyst is the granule that particle diameter is greater than 0.5 millimeter.
The oxide of described transition metal can directly be applied, or is the oxide of carried transition metal, and the carrier of the oxide of this transition metal is active carbon, SiO
2, Al
2o
3or kieselguhr.
The invention provides a kind of way engaged with steam sterilization by ozone sterilization, through actual tests, not only sterilizing thoroughly but also shorten temperature fall time, decrease nutraceutical loss, most importantly greatly reduce energy consumption, reduce the input of factory to steam supply, cooling water system, observable index reduces by 60 ~ 90% with steam sterilization merely, be very easy to industrially extensively promote, fermentation industry will be promoted revolutionary progress occurs.
Detailed description of the invention
Defer to technique scheme, the present embodiment provides a kind of method of fermentable energy-saving sterilizing, specifically carries out according to following steps:
Step one, by the material needed for culture medium in proportion with minimum water dissolution, this ratio adopts 10% ~ 20% of recipe requirements water consumption, adopts the mode of spraying charging culture medium to be squeezed into and is increased in the fermentation tank of 120 ~ 125 DEG C, by steam sterilization sterilizing with steam;
Step 2, requires remaining water in another storage tank with ozone sterilization more than 15 minutes by culture medium prescription;
Step 3, after 30min is placed in pressurize in filtrated air by this water, makes the residue ozone in water decompose by the catalytic decomposition of ozone device with steam sterilization;
Step 4, then the water filtrated air through catalytic decomposition of ozone device removing ozone is pressed into fermentation tank, the temperature of fermentation tank is down to less than 40 DEG C;
Step 5, to the culture medium sample analysis in fermentation tank, can enter follow-up fermentation workshop section after determining the ozone of noresidue in culture medium.
If there is no sugar in the fermentation medium described in step one, a small amount of glucose can be added when not affecting fermentation, the drop ozone that the water that this part glucose thoroughly can decompose injection fermentation tank remains.
Ozone sterilization described in step 2 prepares gaseous ozone with ozonator, then passes into from tank bottoms, or first prepare the ozone water solution of high concentration, add and want in the water of sterilizing.
The thermal creep stress room temperature of the ozone sterilization described in step 2.
The catalyst that catalytic decomposition of ozone device described in step 3 is selected is the oxide of transition metal, and the shape of catalyst is the granule that particle diameter is greater than 0.5 millimeter.
The oxide of described transition metal can directly be applied, or is the oxide of carried transition metal, and the carrier of the oxide of this transition metal is active carbon, SiO
2, Al
2o
3or kieselguhr.
Below provide specific embodiments of the invention, it should be noted that the present invention is not limited to following specific embodiment, all equivalents done on technical scheme basis all fall into protection scope of the present invention.
Embodiment:
200mL culture medium is loaded in the seedlet bottle of 500mL, seedlet bottle is 118 DEG C in temperature, pressure is steam sterilization 20 minutes under the condition of 0.08Mpa, it is 34 DEG C in cultivation temperature, shaking speed is inoculated and cultured in the shaking table of 120 revs/min, about 20 hours of incubation time, is inoculated into off-the-shelf large seed bottle by cultivating complete bacterium liquid in seedlet bottle.
2000mL culture medium is loaded in the large seed bottle of 3000mL, large seed bottle is 121 DEG C in temperature, pressure is steam sterilization 60 minutes under the condition of 0.1Mpa, large seed bottle is 34 DEG C in cultivation temperature, cultivate in the shaking table that shaking speed is 75 revs/min, about 16 hours of incubation time, proceeds to sterilized first class seed pot by cultivating complete bacterium liquid in large seed bottle.
According to the cultural method of this area by the seed culture in 100L first class seed pot, then the secondary seed tank proceeding to 500L is cultivated, and is finally proceeded in fermentation tank by seed after having cultivated.In the process, first class seed pot, secondary seed tank, pipeline, carbon source, nitrogenous source, sour tank, alkali tank, material-compound tank and defoamer tank all adopt steam sterilization.
Consisting of of the culture medium that should add in the fermentation tank of 5000 liters: glucose 100kg, Semen Maydis pulp 50kg, sodium hydrogen phosphate 5kg, potassium dihydrogen phosphate 2.5kg, Chile saltpeter 10kg, magnesium sulfate 2.5kg, sodium chloride 2kg, water 2500kg.The pH value of culture medium is 6.8.
Other raw materials in addition to water in the culture medium of fermentation tank first dissolve in material-compound tank with 500kg water, adopt the mode of spraying charging to squeeze into and be warming up to 121 DEG C of empty fermentation tanks, and steam sterilization 30min at such a temperature; Separately culture medium prescription is required remaining 2000kg water in another storage tank with ozone sterilization more than 15 minutes, this water after pressurize placement 30min, makes the residue ozone in water decompose by the catalytic decomposition of ozone device with steam sterilization by the water after ozone sterilization in filtrated air; Again the water filtrated air through catalytic decomposition of ozone device removing ozone is pressed into fermentation tank, temperature in fermentation tank is down to less than 40 DEG C, adjust the temperature to temperature required again, to the culture medium sample analysis in fermentation tank, follow-up fermentation workshop section after determining the ozone of noresidue in culture medium, can be entered.
From the above, adopt the sterilizing methods in embodiment, only need other raw material raised temperature to 121 DEG C in 500kg water and culture medium, remaining 2000kg water only needs room temperature ozone sterilization, with traditional whole culture medium with compared with the method being steam heated to 121 DEG C of sterilizings, the energy consumption of sterilizing reduces greatly, reduction amplitude reaches 60% ~ 90%, and simultaneously because the concentration of the culture medium of steam sterilization is high, amount is few, spraying charging, sterilizing is thorough; By the temperature that can reduce rapidly with the water injection fermentation tank of ozone sterilization in fermentation tank, reduce consuming time, reduce cooling water amount, and traditional whole culture medium method being steam heated to 121 DEG C of sterilizings, the time of heating in fermentation tank, cooling needs is long.
Claims (6)
1. a method for fermentable energy-saving sterilizing, is characterized in that, specifically carries out according to following steps:
Step one, by the material needed for culture medium in proportion with minimum water dissolution, this ratio adopts 10% ~ 20% of recipe requirements water consumption, adopts the mode of spraying charging culture medium to be squeezed into and is increased in the fermentation tank of 120 ~ 125 DEG C, by steam sterilization sterilizing with steam;
Step 2, requires remaining water in another storage tank with ozone sterilization more than 15 minutes by culture medium prescription;
Step 3, after 30min is placed in pressurize in filtrated air by this water, makes the residue ozone in water decompose by the catalytic decomposition of ozone device with steam sterilization;
Step 4, then the water filtrated air through catalytic decomposition of ozone device removing ozone is pressed into fermentation tank, the temperature of fermentation tank is down to less than 40 DEG C;
Step 5, to the culture medium sample analysis in fermentation tank, can enter follow-up fermentation workshop section after determining the ozone of noresidue in culture medium.
2. the method for fermentable energy-saving sterilizing as claimed in claim 1, it is characterized in that, if there is no sugar in the fermentation medium described in step one, a small amount of glucose can be added, the drop ozone that the water that this part glucose thoroughly can decompose injection fermentation tank remains when not affecting fermentation.
3. the method for fermentable energy-saving sterilizing as claimed in claim 1, it is characterized in that, the ozone sterilization described in step 2 prepares gaseous ozone with ozonator, then passes into from tank bottoms, or first prepare the ozone water solution of high concentration, add and want in the water of sterilizing.
4. the method for fermentable energy-saving sterilizing as claimed in claim 1, is characterized in that, the thermal creep stress room temperature of the ozone sterilization described in step 2.
5. the method for fermentable energy-saving sterilizing as claimed in claim 1, it is characterized in that, the catalyst that the catalytic decomposition of ozone device described in step 3 is selected is the oxide of transition metal, and the shape of catalyst is the granule that particle diameter is greater than 0.5 millimeter.
6. the method for fermentable energy-saving sterilizing as claimed in claim 5, it is characterized in that, the oxide of described transition metal can directly be applied, or is the oxide of carried transition metal, and the carrier of the oxide of this transition metal is active carbon, SiO
2, Al
2o
3or kieselguhr.
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| JP2002369672A (en) * | 2001-06-14 | 2002-12-24 | Yamaya Communications:Kk | Method for sterilizing food by using lactic fermentation liquor |
| CN1426972A (en) * | 2001-12-17 | 2003-07-02 | 李明秀 | Cooling water circulating treatment system and method |
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