MD145Z - Process for the obtaining of a nutrient medium for cultivation of lactobacteria - Google Patents
Process for the obtaining of a nutrient medium for cultivation of lactobacteria Download PDFInfo
- Publication number
- MD145Z MD145Z MDS20090138A MDS20090138A MD145Z MD 145 Z MD145 Z MD 145Z MD S20090138 A MDS20090138 A MD S20090138A MD S20090138 A MDS20090138 A MD S20090138A MD 145 Z MD145 Z MD 145Z
- Authority
- MD
- Moldova
- Prior art keywords
- infusion
- nutrient medium
- lactobacteria
- cultivation
- obtaining
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 235000015097 nutrients Nutrition 0.000 title abstract 3
- 238000001802 infusion Methods 0.000 abstract 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 2
- 230000001954 sterilising effect Effects 0.000 abstract 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 244000205574 Acorus calamus Species 0.000 abstract 1
- 235000006480 Acorus calamus Nutrition 0.000 abstract 1
- 229920001817 Agar Polymers 0.000 abstract 1
- 241000206672 Gelidium Species 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 239000001888 Peptone Substances 0.000 abstract 1
- 108010080698 Peptones Proteins 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 abstract 1
- 235000010419 agar Nutrition 0.000 abstract 1
- 235000021336 beef liver Nutrition 0.000 abstract 1
- 238000009835 boiling Methods 0.000 abstract 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 229940040511 liver extract Drugs 0.000 abstract 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 1
- 235000019341 magnesium sulphate Nutrition 0.000 abstract 1
- 235000007079 manganese sulphate Nutrition 0.000 abstract 1
- 239000011702 manganese sulphate Substances 0.000 abstract 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 abstract 1
- 239000008267 milk Substances 0.000 abstract 1
- 235000013336 milk Nutrition 0.000 abstract 1
- 210000004080 milk Anatomy 0.000 abstract 1
- 235000019319 peptone Nutrition 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 235000017281 sodium acetate Nutrition 0.000 abstract 1
- 239000001632 sodium acetate Substances 0.000 abstract 1
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to microbiology and biotechnology, particularly a process for the obtaining of a nutrient medium for cultivation of lactobacteria.The process includes preparation of the medium containing to 1 L: peptone - 10 g, ammonium citrate - 2 g, sodium acetate - 5 g, glucose - 20.0 g, manganese sulphate crystallohydrate - 0.05 g, magnesium sulphate crystallohydrate - 0.2 g, cystein - 0.2 g, agar-agar - 20 g, Twin-80 - 1 ml, yeast antolysate - 50 ml, beef liver extract - 100 ml, hydrolyzed milk - 500 ml, distilled water - the rest, with a pH 6.2…6.6 and sterilization thereof. In the obtained medium is added sweet calamus rhizome infusion, obtained by infusion of 4.8…7.0 g of chopped raw material in 200 ml of boiling water during 30 minutes with subsequent filtration and sterilization of the obtained infusion, which is added in a quantity of 20 ml to one liter of nutrient medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20090138A MD145Z (en) | 2009-07-22 | 2009-07-22 | Process for the obtaining of a nutrient medium for cultivation of lactobacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20090138A MD145Z (en) | 2009-07-22 | 2009-07-22 | Process for the obtaining of a nutrient medium for cultivation of lactobacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD145Y MD145Y (en) | 2010-02-26 |
| MD145Z true MD145Z (en) | 2010-09-30 |
Family
ID=43568915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20090138A MD145Z (en) | 2009-07-22 | 2009-07-22 | Process for the obtaining of a nutrient medium for cultivation of lactobacteria |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD145Z (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD190Z (en) * | 2009-10-19 | 2010-12-31 | Общественное Учреждение Научно-Практический Институт Садоводства И Пищевых Технологий | Process for the preparation of bacterial leaven for combined lactic-acid products |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1541G2 (en) * | 1998-10-19 | 2001-03-31 | Институт Экологии И Географии Академии Наук Молдовы | Nutritive medium for biphidobacteria biomass accumulation |
| MD2969G2 (en) * | 2004-10-04 | 2006-09-30 | Государственный Университет Молд0 | Process for lactic bacteria cultivation (variants) |
| RU2324732C2 (en) * | 2004-11-24 | 2008-05-20 | Государственное Научное Учреждение Всероссийский Научно-Исследовательский Институт Молочной Промышленности (Гну Вними) | Process of nutrient medium production |
-
2009
- 2009-07-22 MD MDS20090138A patent/MD145Z/en not_active IP Right Cessation
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1541G2 (en) * | 1998-10-19 | 2001-03-31 | Институт Экологии И Географии Академии Наук Молдовы | Nutritive medium for biphidobacteria biomass accumulation |
| MD2969G2 (en) * | 2004-10-04 | 2006-09-30 | Государственный Университет Молд0 | Process for lactic bacteria cultivation (variants) |
| RU2324732C2 (en) * | 2004-11-24 | 2008-05-20 | Государственное Научное Учреждение Всероссийский Научно-Исследовательский Институт Молочной Промышленности (Гну Вними) | Process of nutrient medium production |
Non-Patent Citations (1)
| Title |
|---|
| Timoşco M. Stresul şi flora microbiană intestinală. Chişinău, Tipografia AŞM, 2005, p. 170 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD145Y (en) | 2010-02-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK4Y | Short term patent expired |