LU92320B1 - Mutated DNA polymerases with high selectivity and activity - Google Patents

Mutated DNA polymerases with high selectivity and activity

Info

Publication number
LU92320B1
LU92320B1 LU92320A LU92320A LU92320B1 LU 92320 B1 LU92320 B1 LU 92320B1 LU 92320 A LU92320 A LU 92320A LU 92320 A LU92320 A LU 92320A LU 92320 B1 LU92320 B1 LU 92320B1
Authority
LU
Luxembourg
Prior art keywords
activity
high selectivity
dna polymerases
mutated dna
mutated
Prior art date
Application number
LU92320A
Other languages
English (en)
Inventor
Matthias Drum
Ramon Kranaster
Andreas Marx
Original Assignee
Univ Konstanz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Konstanz filed Critical Univ Konstanz
Priority to LU92320A priority Critical patent/LU92320B1/xx
Priority to US15/100,560 priority patent/US10227643B2/en
Priority to CA2930875A priority patent/CA2930875A1/fr
Priority to PCT/EP2014/076208 priority patent/WO2015082449A2/fr
Priority to EP14825107.7A priority patent/EP3077532B1/fr
Application granted granted Critical
Publication of LU92320B1 publication Critical patent/LU92320B1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
LU92320A 2013-12-02 2013-12-02 Mutated DNA polymerases with high selectivity and activity LU92320B1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
LU92320A LU92320B1 (en) 2013-12-02 2013-12-02 Mutated DNA polymerases with high selectivity and activity
US15/100,560 US10227643B2 (en) 2013-12-02 2014-12-02 Mutated DNA polymerases with high selectivity and activity
CA2930875A CA2930875A1 (fr) 2013-12-02 2014-12-02 Adn-polymerases mutees a haute selectivite et a haute activite
PCT/EP2014/076208 WO2015082449A2 (fr) 2013-12-02 2014-12-02 Adn-polymérases mutées à haute sélectivité et à haute activité
EP14825107.7A EP3077532B1 (fr) 2013-12-02 2014-12-02 Adn-polymérases mutées à haute sélectivité et à haute activité

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LU92320A LU92320B1 (en) 2013-12-02 2013-12-02 Mutated DNA polymerases with high selectivity and activity

Publications (1)

Publication Number Publication Date
LU92320B1 true LU92320B1 (en) 2015-06-03

Family

ID=49841781

Family Applications (1)

Application Number Title Priority Date Filing Date
LU92320A LU92320B1 (en) 2013-12-02 2013-12-02 Mutated DNA polymerases with high selectivity and activity

Country Status (5)

Country Link
US (1) US10227643B2 (fr)
EP (1) EP3077532B1 (fr)
CA (1) CA2930875A1 (fr)
LU (1) LU92320B1 (fr)
WO (1) WO2015082449A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115161302A (zh) * 2021-03-25 2022-10-11 山东大学 一种高特异性Taq DNA聚合酶变体及其获得方法和应用

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101958660B1 (ko) * 2017-07-12 2019-03-18 주식회사 진캐스트 유전자 변이 특이성이 증가된 dna 중합효소의 활성 증가용 pcr 버퍼 조성물
JP6986299B2 (ja) * 2017-07-12 2021-12-22 ジーンキャスト カンパニー リミテッドGenecast Co., Ltd. 遺伝子変異特異性が増加したｄｎａ重合酵素およびその活性増加用ｐｃｒバッファー組成物
KR101958659B1 (ko) * 2017-07-12 2019-03-18 주식회사 진캐스트 유전자 변이 특이적 증폭 효율이 증가된 dna 중합효소
WO2019215065A1 (fr) 2018-05-07 2019-11-14 Roche Innovation Center Copenhagen A/S Procédés de découverte massivement parallèle pour des agents thérapeutiques oligonucléotidiques
CN108588227A (zh) * 2018-07-05 2018-09-28 深圳凌鼎基因科技有限公司 用于结直肠癌诊断的引物及探针、试剂盒及基因检测方法
KR102310819B1 (ko) * 2019-01-11 2021-10-12 주식회사 진캐스트 Tert 돌연변이 검출을 위한 dna 중합효소 및 이를 포함하는 키트
WO2020145711A1 (fr) * 2019-01-11 2020-07-16 주식회사 진캐스트 Adn polymérase pour la détection de mutation d'egfr et trousse comprenant celle-ci
KR102286025B1 (ko) * 2019-01-11 2021-08-05 주식회사 진캐스트 유전자 변이 특이성이 증가된 dna 중합효소를 이용한 질량분석법
KR102678658B1 (ko) 2020-05-29 2024-06-26 (주)제노텍 유전자 변이에 대한 구분성이 향상된 dna 중합효소 변이체
CN115803434A (zh) * 2020-05-29 2023-03-14 基诺泰科有限公司 具有提高的对基因变异的辨别性的dna聚合酶变体
CN116731184B (zh) * 2022-11-25 2024-06-14 厦门康基生物科技有限公司 一种特异性结合Taq酶的单克隆抗体F6H12及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007137700A2 (fr) * 2006-05-30 2007-12-06 Universität Konstanz Adn polymérases mutantes à sélectivité élevée
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
US20110027833A1 (en) * 2009-07-31 2011-02-03 Holly Hogrefe Thermostable type-a dna polymerase mutants with increased polymerization rate and resistance to inhibitors

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007137700A2 (fr) * 2006-05-30 2007-12-06 Universität Konstanz Adn polymérases mutantes à sélectivité élevée
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
US20110027833A1 (en) * 2009-07-31 2011-02-03 Holly Hogrefe Thermostable type-a dna polymerase mutants with increased polymerization rate and resistance to inhibitors

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KATSUSHI YOSHIDA ET AL: "Arg660Ser mutation in Thermus aquaticus DNA polymerase I suppresses T to C transitions: implication of wobble base pair formation at the nucleotide incorporation step", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 29, no. 20, 1 October 2001 (2001-10-01), pages 4206 - 4214, XP008140680, ISSN: 0305-1048, DOI: 10.1093/NAR/29.20.4206 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115161302A (zh) * 2021-03-25 2022-10-11 山东大学 一种高特异性Taq DNA聚合酶变体及其获得方法和应用
CN115161302B (zh) * 2021-03-25 2023-08-29 山东大学 一种高特异性Taq DNA聚合酶变体及其获得方法和应用

Also Published As

Publication number Publication date
EP3077532B1 (fr) 2019-06-19
US10227643B2 (en) 2019-03-12
WO2015082449A2 (fr) 2015-06-11
CA2930875A1 (fr) 2015-06-11
US20160298174A1 (en) 2016-10-13
EP3077532A2 (fr) 2016-10-12
WO2015082449A3 (fr) 2015-08-27

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